Application of nicotinamide mononucleotide and/or nicotinamide mononucleotide salt
A single nucleotide salt and single nucleotide technology, applied in the application field of nicotinamide mononucleotide and/or nicotinamide mononucleotide salt, can solve adverse reactions, easy recurrence, allergies requiring repeated medication, etc. problems, to achieve the effects of fewer adverse reactions, reduced sIgE synthesis, and reduced allergic recurrence rate
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Embodiment 1
[0032] In this example, C3H / HeJ peanut-allergic mice were prepared by the following method: 6-week-old female C3H / HeJ mice were purchased from Shanghai Experimental Animal Center. The test animals are reared under specific non-pathogenic conditions in a room with an average temperature of 21° C. to 23° C. and a relative humidity of 40% to 70%, with a 12-hour light / dark cycle. The mice in the sensitization group were gavaged with peanut homogenate (single 80mg / each) every day, and on the 21st day after the implementation of the sensitization measures, the mice were given intraperitoneal injection (i.p) injection of 30mg CPE (peanut extract) / hour Rats, and young rats that had never been exposed to peanuts were used as negative controls. Then, by skin testing and anaphylaxis (by measuring vascular leak, monitoring clinical symptoms, rectal temperature, respiratory rate, and measuring serum mast cell protease-1 (mmcp-1) - a specific marker of mast cell degranulation) The assay co...
Embodiment 2
[0052] C3H / HeJ peanut-allergic mice in the test example of the present invention were prepared by the following method: 6-week-old female C3H / HeJ mice were purchased from Shanghai Experimental Animal Center. The test animals are reared under specific non-pathogenic conditions in a room with an average temperature of 21° C. to 23° C. and a relative humidity of 40% to 70%, with a 12-hour light / dark cycle. The mice in the sensitization group were gavaged with peanut homogenate (single 80mg / each) every day, and on the 21st day after the implementation of the sensitization measures, the mice were given intraperitoneal injection (i.p) injection of 30mg CPE (peanut extract) / hour Rats, and young rats that had never been exposed to peanuts served as negative controls. Then, by skin testing and anaphylaxis (by measuring vascular leak, monitoring clinical symptoms, rectal temperature, respiratory rate, and measuring serum mast cell protease-1 (mmcp-1) - a specific marker of mast cell deg...
Embodiment 3
[0072] 3.1 Experimental materials and experimental reagents:
[0073] The peripheral blood of healthy children of the same age and the peripheral blood (PBMC) of children with food allergy were obtained from Shenzhen Children's Hospital. IL10, TGF-β, IFN-γ and IgE antibodies (purchased from Sigma-aldrich), NMN (purchased).
[0074] 3.2 Experimental method
[0075] The peripheral blood (PBMC) of healthy children and food-allergic children were randomly collected, and the PBMCs were suspended in a medium (RPMI-1640; Mediatech) with 10% autologous plasma, and placed in a 37°C, 5% CO2 cell culture incubator. The training groups are as follows:
[0076] ①. Healthy group: PBMC (10^7 / ml) of healthy children were suspended in culture medium (0.2 mg / ml of physiological saline was added to the medium);
[0077] ②. Blank group: PBMC (10^7 / ml) from children with food allergy were suspended in culture medium (0.2 mg / ml of normal saline was added to the medium);
[0078] ③. Treatment gr...
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