The invention discloses a traditional Chinese medicine air disinfectant for a clinical laboratory and a preparation method thereof, and belongs to the field of traditional Chinese medicine. The medicine effective components of the air disinfectant comprise the following raw materials: pyrola calliantha, folium mori, haliotidis concha, eupatorium fortune, pyrrosia lingua, dendranthema indicum, gentiana scabra, glycyrrhiza uralensis, achyranthes aspera, dark plum, plantago asiatica, fructus forsythia, adina pilulifera, agastache rugosa, rhus chinensis, atractylodes rhizome, folium artemisiae argyi, platycladus orientalis, coriandrum sativum, piper longum, cortex moutan radicis, rubia cordifolia, rosmarinus officinalis and aristolochia debilis. The air disinfectant belongs to a pure traditional Chinese medicine material preparation, is poisonless and harmless, has obvious bacteriostatic or bactericidal effect on staphylococcus aureus, pneumococcus, mycobacterium tuberculosis, typhoid bacillus, corynebacterium diphtheria, escherichia coli and pseudomonas aeruginosa, has a certain inhibiting effect on other pathogenic bacteria, influenza virus and fungi, is good in sterilization effect,does not have stimulation for human body, does not have extraneous odor and has vegetation fragrance, can perform refreshment and purify and refresh the air, and does not affect the normal work of inspection personnel after sterilization.