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Pastevula mulfocida capsular polysaccharide-protein conjugate vaccine and preparation method thereof

A technology of protein-conjugated vaccine and Pasteurella avian, applied in the biological field, can solve the problems of multiple vaccinations, high preparation costs, and complicated operations, and achieve the effects of cost saving, enhanced vaccine virulence, and simple operation

Active Publication Date: 2018-11-02
广州渔跃生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Chinese patent application CN105288607A discloses a preparation method of an inactivated vaccine for avian pasteurellosis. The inactivated vaccine for avian pasteurellosis prepared by the method is effective in immunizing rabbits. However, in the process of using Among them, this inactivated vaccine has a short action time in the body, requires multiple vaccinations, and is time-consuming and laborious to use.
However, the preparation method is complicated to operate, requires strict operation procedures, and the preparation cost is relatively high.

Method used

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  • Pastevula mulfocida capsular polysaccharide-protein conjugate vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, a kind of Pasteurella avian capsular polysaccharide-protein conjugate vaccine

[0030] The Pasteurella avian capsular polysaccharide-protein conjugate vaccine is formed by connecting the Pasteurella avian capsular polysaccharide with a diphtheria toxoid carrier protein through nanometer microspheres. The mass ratio of the Pasteurella avian capsular polysaccharide, nano microspheres and diphtheria toxoid carrier protein is 15:18:25.

[0031] The preparation method is as follows:

[0032] S1. Weigh the biopolymer material used for the preparation of nano-microspheres with a weight of 1.2 times of the capsular polysaccharide and dissolve it in dichloromethane of 3 times the weight, then add it into an aqueous solution of polyvinyl alcohol with a mass concentration of 1%, and stir to dissolve , to obtain a mixed solution with a final concentration of biopolymer material of 0.1 mg / mL;

[0033] S2, emulsify the mixed solution prepared in step S1 under the cond...

Embodiment 2

[0039] Embodiment 2, a kind of Pasteurella avian capsular polysaccharide-protein conjugate vaccine

[0040] The Pasteurella avian capsular polysaccharide-protein conjugate vaccine is formed by connecting the Pasteurella avian capsular polysaccharide with a diphtheria toxoid carrier protein through nanometer microspheres.

[0041] The mass ratio of the Pasteurella avian capsular polysaccharide, nano microspheres and diphtheria toxoid carrier protein is 10:15:4.

[0042] The preparation method is as follows:

[0043] S1, weighing 1.5 times the weight of the capsular polysaccharide to prepare the biopolymer material used for the preparation of the nanospheres and dissolving it in 3-5 times the weight of methylene chloride, and then adding it into an aqueous solution of polyvinyl alcohol with a mass concentration of 10%, Stir and dissolve to obtain a mixed solution with a final concentration of the biopolymer material of 5 mg / mL;

[0044] S2, emulsifying the mixed solution prepa...

Embodiment 3

[0050] Embodiment 3, a kind of Pasteurella avian capsular polysaccharide-protein conjugate vaccine

[0051] The invention relates to a Pasteurella avium capsular polysaccharide-protein conjugate vaccine, wherein the Pasteurella avian capsular polysaccharide-protein conjugate vaccine is formed by connecting the Pasteurella avian capsular polysaccharide with a diphtheria toxoid carrier protein through nanometer microspheres. The mass ratio of the Pasteurella avian capsular polysaccharide, nano microspheres and diphtheria toxoid carrier protein is 15:21:10.

[0052] The preparation method is as follows:

[0053] S1. Weigh the biopolymer material used for the preparation of nano microspheres with a weight of 1.4 times of the capsular polysaccharide and dissolve it in 4 times of the weight of methylene chloride, then add it into an aqueous solution of polyvinyl alcohol with a mass concentration of 7%, stir and dissolve , to obtain a mixed solution with a final concentration of the...

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Abstract

Belonging to the technical field of biology, the invention in particular relates to a pastevula mulfocida capsular polysaccharide-protein conjugate vaccine and a preparation method thereof. The pastevula mulfocida capsular polysaccharide-protein conjugate vaccine provided by the invention is formed by connection of pastevula mulfocida capsular polysaccharide and diphtheria toxoid carrier protein through nano-microspheres. The combination of pastevula mulfocida capsular polysaccharide with the carrier protein diphtheria toxoid enhances the immune efficacy of the vaccine, and also reaches a cross protection effect on the infection of homologous strains and heterologous strains. The preparation method of the pastevula mulfocida capsular polysaccharide-protein conjugate vaccine provided by theinvention has the characteristics of simple operation and cost saving, and has good application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a Pasteurella avian capsular polysaccharide-protein conjugate vaccine and a preparation method thereof. Background technique [0002] Pasteurella multocida, referred to as Pasteurella for short, is a short Gram-negative bacillus with capsules that can infect many animals such as livestock, poultry and wild birds. Clinical studies have shown that fowl cholera is a contagious disease mainly caused by serotype A:1, A:3 or A:4 Pasteurella, which causes serious economic losses to the poultry industry. [0003] Chinese patent application CN105288607A discloses a preparation method of an inactivated vaccine for avian pasteurellosis. The inactivated vaccine for avian pasteurellosis prepared by the method is effective in immunizing rabbits. However, in the process of using Among them, this inactivated vaccine has a short action time in the body and needs multiple vaccinations, wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/385A61K39/102A61P31/04
CPCA61K39/102A61K39/385A61K2039/522A61K2039/6037A61K2039/627A61P31/04
Inventor 严悌昆张毓金谢秉超杨球马军
Owner 广州渔跃生物技术有限公司
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