2 results about "Diptheria toxoid" patented technology

The application discloses a preparation method and application of a cocktail vaccine of Acinetobacter baumannii, and belongs to the technical field of vaccine preparation. The vaccine comprises sugar conjugates formed by coupling three branched pentasaccharides of K14, K47 and K128 with diphtheria toxoid. The preparation method comprises the steps of hapten synthesis, purification, coupling with carrier protein and mixing. Experiments show that the vaccine can induce high levels of specific antibodies, significantly reduce the tissue bacterial load of mice infected with Acinetobacter baumannii, and has a wide protective effect. The application is suitable for preventing and treating various infectious diseases caused by Acinetobacter baumannii.

The invention belongs to the technical field of biological medicines, and particularly discloses an acinetobacter baumannii capsular polysaccharide-protein conjugate as well as a preparation method and application thereof. The acinetobacter baumannii capsular polysaccharide repetitive unit is used as a target product, and the specific compound is synthesized by 12 steps of reaction, including adding corresponding catalysts into different compounds in a specific solvent for reaction, and performing separation, purification and other operations. And carrying out deprotection and other treatments on the product to finally obtain a product q. The acinetobacter baumannii capsular polysaccharide-protein conjugates r and s are obtained by synthesizing pentasaccharides, then covalently linking the synthesized pentasaccharides with bovine serum albumin (BSA) and diphtheria toxoid (DT) through 3, 4-diethoxy-3-cyclobutene-1, 2-diketone respectively, and obtaining the acinetobacter baumannii capsular polysaccharide-protein conjugates r and s. According to the developed chemical total synthesis route, the oligosaccharide fragments chemically synthesized through the technical route have the advantages of being high in purity and clear in chemical structure and do not contain potential microbial pollutants, and meanwhile oligosaccharide fragments of different lengths can be controllably synthesized.