Preparation method of diphtheria toxoid vaccine

A technology for diphtheria toxoid and vaccine, which is applied in the field of preparation of diphtheria toxoid vaccine, can solve the problems of simple and extensive, large shear force of bacteria and low quality, and achieves the advantages of reducing antigen damage, reducing pore blockage and improving production efficiency. Effect
CN102961740AActive Publication Date: 2013-03-13WUHAN INST OF BIOLOGICAL PROD CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUHAN INST OF BIOLOGICAL PROD CO LTD
Publication Date
2013-03-13

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Abstract

The invention discloses a preparation method of a diphtheria toxoid vaccine. The diphtheria toxoid vaccine is prepared by taking a diphtheria bacillus strain as a raw material and then carrying out the steps of diphtheria toxoid culture, bacteria-liquid separation, ultrafiltration and concentration, two-time salting out, ultrafiltration and desalination and the like. According to the preparation method, the thalli of the culture solution is removed by using a hollow fiber filter membrane and then refining is carried, so that the pore blockage caused by the accumulation of the thalli and other impurity fragments in the subsequent filter process is prevented; through an improved salting out method, the toxin proteins and other allergens in the culture solution are effectively removed; and a tangential flow ultrafiltration method is used for both the concentration of the culture solution and the desalting method after the salting out, so that the antigen damage caused by the shearing to the toxin proteins is decreased and the precipitation of the proteins is avoided. According to the preparation method, the preparation time of the diphtheria toxoid vaccine is shortened and the production efficiency is improved.
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Description

technical field

[0001] The invention relates to a preparation method of a diphtheria toxoid vaccine. Background technique

[0002] The diphtheria toxoid vaccine is made from diphtheria bacillus. The toxin secreted by the diphtheria bacillus is a single polypeptide chain without sugar groups and prosthetic groups. The protein is composed of 560 amino acids and has a molecular weight of 62kDa. It is a zymogen , Only under the action of protease, it can be cut into the gap toxin, it has biological activity. The isoelectric point is about pH4.1. At pH6, it will lose its activity and solidify quickly when heated to 55°C. Diphtheria toxoid includes two fragments, A and B, among which the B fragment is a receptor-binding and membrane-penetrating fragment, and the A fragment is a toxic fragment, which finally causes cell death by inhibiting the enzyme activity in protein synthesis. It can also be toxic to distal tissues and organs, especially the heart and the peripheral and centr...

Claims

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