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Production of diphtheria toxin

A technology of diphtheria toxin and diphtheria stick, which is used in medical preparations containing active ingredients, bacterial antigen components, depsipeptides, etc.

Inactive Publication Date: 2007-01-10
圣诺菲·帕斯图尔公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although bacteria grow well in it, production of diphtheria toxoid is minimal

Method used

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  • Production of diphtheria toxin
  • Production of diphtheria toxin
  • Production of diphtheria toxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] Preparation of Amino Acid Medium Without Phytochemicals

[0028] NZ Amine is a source of amino acids and peptides prepared from enzymatic digestion of casein. It is a good source of both amino nitrogen (free amino acids) and organic nitrogen (peptides). Another source of amino acids and peptides in the Corynebacterium diphtheriae medium for the preparation of diphtheria toxoid is Toxiprotone-D of animal origin. The composition of these media is shown in the table and below:

[0029] Composition of media containing NZ Amine

[0030] Element

amount per liter

NZ Amine

30g

Acetic acid

7.2mL

Maltose

25g

growth factor

8mL

10% L-cystine

2mL

60% sodium lactate

1.7mL

pH

7.5

[0031] Element

quantity

magnesium sulfate

225g

beta alanine

2.30g

Pimelic acid

0.15g

Zinc sulfate

0.80g

copper sulfate

0.50g

Manga...

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Abstract

A Corynebacterium diphtheriae culture medium for the production of diphtheria toxin and methods for producing the toxin are provided. The medium is substantiallty free of animal-derived products and comprises water, a carbohydrate source, a nitrogen source and a number of free amino acids in an initial concentration wherein the initial concentration of each free amino acid is not limiting for the production of the toxin.

Description

field of invention [0001] The invention relates to a bacterial growth medium for preparing diphtheria toxin and a preparation method of diphtheria toxin. Background of the invention [0002] Diphtheria is a life-threatening disease caused by infection with Corynebacterium diphtheriae, a Gram-positive, aerobic, rod-shaped bacterium. The disease is caused by a local attack of the nasopharyngeal tissue by a toxigenic strain of Corynebacterium diphtheriae. The organism grows within a rough fibrin membrane covering sore, hemorrhagic, and necrotic lesions, which may be located in the tonsil or nasopharyngeal region. During a typical epidemic in the past, the spread of the disease was through droplet transmission. Unless aggressively treated with antibiotics, a recovering diphtheria patient may harbor toxigenic bacteria in the larynx and nasopharynx for weeks or months. [0003] Most of the clinical symptoms of diphtheria are caused by strong diphtheria toxin produced by Coryneb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P21/00A61K39/05A61P31/04A61K39/00C07K14/34
CPCC12N1/20C07K14/34A61K39/00A61P31/04A61P37/04
Inventor T·李X·余
Owner 圣诺菲·帕斯图尔公司
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