Production of diphtheria toxin
A technology of diphtheria toxin and diphtheria stick, which is used in medical preparations containing active ingredients, bacterial antigen components, depsipeptides, etc.
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[0027] Preparation of Amino Acid Medium Without Phytochemicals
[0028] NZ Amine is a source of amino acids and peptides prepared from enzymatic digestion of casein. It is a good source of both amino nitrogen (free amino acids) and organic nitrogen (peptides). Another source of amino acids and peptides in the Corynebacterium diphtheriae medium for the preparation of diphtheria toxoid is Toxiprotone-D of animal origin. The composition of these media is shown in the table and below:
[0029] Composition of media containing NZ Amine
[0030] Element
amount per liter
NZ Amine
30g
Acetic acid
7.2mL
Maltose
25g
growth factor
8mL
10% L-cystine
2mL
60% sodium lactate
1.7mL
pH
7.5
[0031] Element
quantity
magnesium sulfate
225g
beta alanine
2.30g
Pimelic acid
0.15g
Zinc sulfate
0.80g
copper sulfate
0.50g
Manga...
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