Method for extracting DNA by echinoderm living body sampling

Abstract

The invention discloses a method for sampling and extracting DNA from a living body of echinoderm. The tube feet of a living sea urchin or the tube feet, the tentacles or the intestines of a living sea cucumber are taken to be lysed and digested by lysis solution provided with DNA enzyme, and after the steps of extraction, centrifugal supernatant fluid taking, centrifugal deslagging (sclerite), ethyl alcohol precipitation, centrifugal separation, detection, etc., the concentration and the mass of the extracted DNA are consistent with the concentration and the mass of the DNA extracted from the traditional (dead body) sample tissue of the echinoderm. The echinoderm is not required to be killed, but is sampled from the living body to extract the DNA, so that the molecular biology technologycan really become an auxiliary mean of genetic breeding. By using the invention, the echinoderm resources can be rationally used for the works of auxiliary breeding, cultivation condition monitoring,genetic screening, genealogy identification, etc. In addition, the invention also has great significance for the protection and the research of various endangered species in the echinoderm.

Description

Technical field:

[0001] The invention relates to a method for extracting DNA from echinoderms, in particular to a method for extracting DNA from living samples of echinoderms. Background technique:

[0002] At present, animal DNA extraction technology has been widely used in genetic breeding, experimental research, and production testing. The basic method is to remove some tissue samples from the animal body (living body, dead body), and fully shred or homogenize the sample in the lysate. Slurry, and fully digest the sample tissue, and then go through the steps of extraction, centrifugation to get the supernatant, ethanol precipitation, centrifugation, detection and other steps, and the living sampling is more conducive to genetic breeding, breeding status monitoring, gene screening, family identification, etc. Research. Some marine animals can be sampled in vivo, such as fish, which can draw blood, cut fin rays, etc., but it is difficult to achieve for echinoderms (sea urc...