Fucoidan sulfate and preparation method thereof

Abstract

The invention discloses a method for preparing oligomeric fucoidan sulfate, which comprises the steps of using dry echinoderm as raw material, extracting supernatant by alkali hydrolysis and enzymolysis; adjusting the pH value of the supernatant to precipitate protein, centrifuging, salting Crude polysaccharides were obtained through ethanol precipitation and ethanol precipitation; fucoidan sulfate was obtained through ethanol fractional precipitation and ultrafiltration; the peroxide degradation catalyzed by the catalyst formed by the transition metal ion of the fourth period of the periodic table of elements or acid degradation was used to obtain oligomerization Fucoidan sulfate; Wherein, the weight-average molecular weight of described oligomeric fucoidan sulfate is 8-140kDa, and its structural characteristic is to comprise 20%-40% of the fucose of 40%-60% at least Sulfate groups.

Description

technical field

[0001] The invention belongs to the technical field of medicine, in particular to a method for preparing fucoidan sulfate and a product obtained by the method.

[0002] technical background

[0003] Fucoidan sulfate (Fucoidan) is a class of polysaccharides mainly composed of fucose and sulfate ester groups, widely present in a variety of brown algae and some marine invertebrates (such as sea cucumbers, sea urchins, etc.). In 1913, Kylin first extracted fucoidan sulfate from the brown alga Laminaria digitata. A large number of studies have confirmed that fucoidan sulfate has various biological activities such as anticoagulation, lowering blood fat, antioxidation, antitumor, and enhancing the body's immune function.

[0004] Although there are many studies on the structure of fucoidan sulfate, the fine structure of most fucoidan sulfate has not been elucidated due to its irregular structure and often branched structure. There are obvious inter-species differen...

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