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Fucoidan sulfate and preparation method thereof

A technology of polyfucoidan sulfate and conditions, which is applied in the field of preparation of fucoidan sulfate, can solve the problems of increasing the difficulty of separation and purification of degradation products, high degradation temperature, and complicated enzyme screening process, etc. The effect of high content of algalose and sulfate ester groups, high product purity, and mild conditions

Active Publication Date: 2017-07-07
SHENZHEN NEPTUNUS PHARMA RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cost of enzymatically degrading polysaccharides is high, there is no specific degrading enzyme, and the process of screening enzymes is also a relatively complicated problem
Chemical degradation methods, such as oxidative degradation methods, the traditional hydrogen peroxide degradation method uses a large amount of hydrogen peroxide, which increases the difficulty of separation and purification of degradation products, and the degradation temperature required for the reaction is relatively high, which is likely to cause discoloration of the product and affect the appearance of the product
[0009] There are deficiencies in the existing extraction, separation and purification, and degradation technologies of fucoidan sulfate, which restrict the research and utilization of fucoidan sulfate to a certain extent

Method used

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  • Fucoidan sulfate and preparation method thereof
  • Fucoidan sulfate and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] [Example 1] Preparation of Fucoidan Sulfate

[0026] Take 1 kg of dried plum blossom slices (about 1.5 mm in thickness), put them into a reaction tank (25 L), add 8 L of 0.5 mol / L NaOH solution, and stir and extract at 40 ° C for 3 hours. After cooling, adjust the pH value to 6-7 with 6mol / L hydrochloric acid, add 7L papain aqueous solution (containing papain 5g, 8×10 5 U / g), stirred at 50°C for 6 hours, then raised to 100°C for 10 minutes to inactivate the enzyme. Cool, and centrifuge to remove the precipitate. The resulting supernatant was adjusted to pH 2.5 with 6 mol / L hydrochloric acid (precipitated protein), allowed to stand at 4°C for 4 hours, and centrifuged to remove the precipitate. Adjust the supernatant to neutral, add potassium acetate to make the final concentration 1mol / L, and add ethanol to 60% (v / v), let it stand, and centrifuge to collect the precipitate (crude polysaccharide of plum flower ginseng). Dissolve the obtained precipitate in 5L of water,...

Embodiment 2

[0027] [Example 2] Preparation of oligomeric fucoidan sulfate

[0028] Hydrogen peroxide oxidative degradation of plum flower ginseng fucoidan sulfate: take 1.0 g of FUC prepared in Example 1, 2.72 g of sodium acetate trihydrate and 1.17 g of sodium chloride and dissolve them in 38 mL of water, add 2 mL of 80 mmol / L Copper acetate solution, stir to mix. Under the condition of stirring in a water bath at 35°C, the pH of the reaction was controlled to 7.5, and 10% (v / v) H 2 o 2The degradation reaction was 3 hours. Add 0.1g Na 2 EDTA terminates the reaction. The reaction solution was concentrated under reduced pressure, and 2.5 times the volume of 95% ethanol was added for precipitation, left to stand, and centrifuged to collect the precipitate. The precipitate was dissolved in water, dialyzed with a dialysis bag with a molecular weight cut-off of 1000Da, and freeze-dried to obtain 0.68 g of oligomeric plum flower ginseng fucoidan sulfate (dFUC-1).

Embodiment 3

[0029] [Example 3] Preparation of oligomeric fucoidan sulfate

[0030] Hydrogen peroxide oxidative degradation of plum flower ginseng fucoidan sulfate: take 1.0 g of FUC prepared in Example 1, and degrade it according to the method of Example 2, H 2 o 2 Degradation reaction 6 hours. The post-treatment method is the same as in Example 2, and finally 0.47 g of oligomeric plum flower ginseng fucoidan sulfate (dFUC-2) is obtained.

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Abstract

The invention discloses a method for preparing oligomeric fucoidan sulfate, which comprises the steps of using dry echinoderm as raw material, extracting supernatant by alkali hydrolysis and enzymolysis; adjusting the pH value of the supernatant to precipitate protein, centrifuging, salting Crude polysaccharides were obtained through ethanol precipitation and ethanol precipitation; fucoidan sulfate was obtained through ethanol fractional precipitation and ultrafiltration; the peroxide degradation catalyzed by the catalyst formed by the transition metal ion of the fourth period of the periodic table of elements or acid degradation was used to obtain oligomerization Fucoidan sulfate; Wherein, the weight-average molecular weight of described oligomeric fucoidan sulfate is 8-140kDa, and its structural characteristic is to comprise 20%-40% of the fucose of 40%-60% at least Sulfate groups.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular to a method for preparing fucoidan sulfate and a product obtained by the method. [0002] technical background [0003] Fucoidan sulfate (Fucoidan) is a class of polysaccharides mainly composed of fucose and sulfate ester groups, widely present in a variety of brown algae and some marine invertebrates (such as sea cucumbers, sea urchins, etc.). In 1913, Kylin first extracted fucoidan sulfate from the brown alga Laminaria digitata. A large number of studies have confirmed that fucoidan sulfate has various biological activities such as anticoagulation, lowering blood fat, antioxidation, antitumor, and enhancing the body's immune function. [0004] Although there are many studies on the structure of fucoidan sulfate, the fine structure of most fucoidan sulfate has not been elucidated due to its irregular structure and often branched structure. There are obvious inter-species differen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P7/02
Inventor 王宇陈艳萍谭道鹏冯贻东严启新冯汉林
Owner SHENZHEN NEPTUNUS PHARMA RES INST CO LTD
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