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Method for producing microorganism culture media nitrogen source from waste organisms

A microbial culture medium and organic matter technology, applied in fungi, bacteria and other directions, can solve the problems of low calorific value of biological organic matter, limited application prospects, accumulation of antibiotics, etc., and achieve the effects of stable physical and chemical properties, wide source of raw materials, and simple process route.

Inactive Publication Date: 2011-08-10
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biological organic matter is the main waste in the production of fermentation industry, which contains a large amount of bacterial protein (accounting for more than 40% of the dry weight), rich vitamins, growth factors and culture medium residues and other nutrients, and there are also microorganisms Various toxic metabolites and downstream processing additives are usually treated by incineration, used as feed, fertilizer, etc. Biological organic matter has the disadvantage of low calorific value, and high-quality heat will not be produced after incineration, and biological Organic matter contains N, S and other elements, and incineration will produce a large amount of toxic substances, causing serious environmental pollution and limiting its application prospects; the existence of trace amounts of antibiotic ingredients, after being used to feed livestock and poultry or applied to farmland, It will inevitably cause the accumulation of antibiotics in environmental organisms, reduce the therapeutic effect of antibiotics, and have serious consequences
Biological organic matter contains extremely rich organic matter. If it is not treated in time, it will form secondary fermentation, produce foul smell, and cause environmental pollution.
[0004] There are a few manufacturers in China that use direct drying to produce feed protein. This simple physical drying method cannot actually remove the above poisons

Method used

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  • Method for producing microorganism culture media nitrogen source from waste organisms
  • Method for producing microorganism culture media nitrogen source from waste organisms
  • Method for producing microorganism culture media nitrogen source from waste organisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Add 500ml of 0.01M NaOH to 50g of wet citric acid waste mycelia, heat the reaction at 80°C for 0.5h, filter, remove the precipitate, add 0.1ml of 1MH to the protein-rich phase 2 SO 4 Buffer, adjust the pH to 4, add 0.5ml, 0.01M cellulase, and hydrolyze at 80°C for 0.5 hours, and the highest absorbance is detected, that is, the reaction is complete. The reaction solution obtained above is hydrolyzed, then decolorized and concentrated, dried in an oven at 90°C, and crushed into powder after drying, which is the product.

[0025] After the product is processed, it is used for microbial culture, and the culture results are as follows:

[0026] The product was used for the cultivation of Escherichia coli, and the bacterial liquid culture medium (Table 1) was used to replace the beef extract in Table 1 as a nitrogen source, and it was cultivated at 37°C for 24 hours, and a good cultivation effect was obtained.

[0027] The product was used for the cultivation of Escherichia...

Embodiment 2

[0032] The operating methods of embodiment 2, embodiment 3 and embodiment 4 are the same as embodiment 1, and the operating parameters are shown in table 1.

Embodiment 5

[0034] Add 50ml of 1M KOH to 50g of waste bean curd, heat and react at 40°C for 12h, filter, remove the precipitate, add a certain amount of 0.01M acetic acid to the protein-rich phase for buffering, adjust the pH to 10, and then add 5ml, 1M Hydrochloric acid is hydrolyzed at a temperature of 20°C for 12 hours, and the highest absorbance is detected through detection, that is, the reaction is complete. The reaction solution obtained above is hydrolyzed, then decolorized and concentrated, dried in an oven at 60°C, and crushed into powder after drying, which is the product.

[0035]After the product is processed, it is used for microbial culture, and the culture results are as follows:

[0036] The product was used for the cultivation of Escherichia coli, using the bacterial liquid culture medium (Table 2) instead of the beef extract in Table 2 as the nitrogen source, and culturing at 37°C for 24 hours, and a good cultivation effect was obtained.

[0037] The product was used f...

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Abstract

The invention relates to a method for producing microorganism culture media nitrogen source from waste organisms, belonging to the field of reutilizing the waste resource to produce the microorganism culture media nitrogen source. The microorganism culture media nitrogen source is produced according to the following steps: pretreating the waste organisms are pretreated, then collecting the protein liquid obtained after the reaction of the pretreated waste organisms and processing solution, and hydrolyzing the protein liquid in a certain condition, decolorizing, concentrating and drying. The microorganism culture media nitrogen source is consistent with the microorganism culture media nitrogen source sold on the market in the superficial morphology, and the accurate analysis proves that the microorganism culture media nitrogen source contains various necessary and unnecessary amino acids which can provide enough nutrients for the growth of the microorganisms and ensure the normal growth of the microorganisms and contains no residual matters which can affect the growth of the microorganisms. The microorganism culture media nitrogen source can be used for culturing various solid and liquid microorganisms with better effect.

Description

technical field [0001] The invention relates to a method for preparing a nitrogen source that can be used as a microbial culture medium. The core material is the waste bio-organic matter produced by the fermentation industry. The nitrogen source can be used for liquid and solid state cultivation of various microorganisms. Background technique [0002] The medium used in the cultivation of microorganisms such as bacteria and fungi is mainly composed of nitrogen source, carbon source, inorganic salts, growth factors and water. In the process of microbial cultivation, the addition of nitrogen source plays a key role. The substances commonly used as nitrogen source include beef extract, peptone and yeast powder. Beef extract is a tan to tan paste obtained from fresh beef after removing fat, digesting, filtering, and concentrating. Peptone is a water-soluble mixture of peptides and amino acids obtained by enzymatic, acid, and alkali hydrolysis of proteins. Yeast extract powde...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14
Inventor 苏海佳韩庆
Owner BEIJING UNIV OF CHEM TECH
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