A natural non-toxic fluorescent protein dye from a non-bioluminescent marine invertebrate, compositions containing the said dye and its uses

A technology of fluorescent dyes and compositions, applied in the field of natural non-toxic multicolor fluorescent protein dyes

Inactive Publication Date: 2005-08-03
科学和工业研究委员会
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0042] The currently reported dye is also a natural dye, but it is a non-toxic multicolor fluorescent protein extracted directly from the ovarian cells of non-biofluorescent invertebrates

Method used

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  • A natural non-toxic fluorescent protein dye from a non-bioluminescent marine invertebrate, compositions containing the said dye and its uses
  • A natural non-toxic fluorescent protein dye from a non-bioluminescent marine invertebrate, compositions containing the said dye and its uses
  • A natural non-toxic fluorescent protein dye from a non-bioluminescent marine invertebrate, compositions containing the said dye and its uses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0331] Embodiment 1: collect raw material

[0332] The material in this patent is sea cucumber and has the following taxonomic details

[0333] Subkingdom: Metazoa

[0334] Phylum: Echinodermata

[0335] Subphylum: Migratory Echinodermata

[0336] Class: sea cucumber class

[0337] Subclass: Aspidochrotacea

[0338] Order: Aspidochirota

[0339] Genus: Sea Cucumber

[0340] Species: Scabra

[0341] Material was collected from the west and central coast of India at low tide. Take it to the laboratory and place it in a glass jar filled with seawater with a salinity of 30-32 / par (30‰) before use.

Embodiment 2

[0343] Isolation of dyes from female gonads

[0344] Animals were washed first with tap water and then with ultrapure water. Carcasses were cut open with a quick shear, and male / female animals were sorted. The female gonads are identified and carefully removed from the evisceration. The color of the ovary varies from yellow to orange depending on how mature or semi-mature the ovary cells are. Weigh the ovarian tissue and place in a glass beaker. 70% ethanol was added in a ratio of 1:3 (weight to volume ratio). A yellowish-orange pigment appears. The colored solution was transferred to other containers. 70% ethanol was added again to the remaining tissue and the colored solution was removed. These steps were repeated three times to extract the pigment without homogenizing the ovarian tissue. Partial purification of the dye

[0345] The extract was partially purified by filtration through whattman No. 1 filter paper. The screw-cap vial containing the dye solution was la...

Embodiment 3

[0347] Example 3: Physical Properties of Dye Color and Solubility

[0348] The dye solution is pale yellow-orange in visual inspection. It produces a changing color emission under daylight / tube lighting. Dyes are insoluble in water but soluble in alcohol.

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Abstract

The present invention discloses a process of extraction, purification and characterization of a natural eco-friendly nontoxic cell permeant multiple fluorescent protein dye from a non bioluminescent marine echinoderm Holothuria scabra, compositions containing the dye and various applications of the dye.

Description

technical field [0001] The invention relates to a natural non-toxic multicolor fluorescent protein dye extracted from the ovary tissue of the marine invertebrate (Holothuria scabra). The present invention also provides a method for extracting, partially purifying and identifying the new natural dye. Nontoxic, cell membrane permeable, lectin-binding, ease of insemination and fertilization properties have also been reported in marine animals. The marine invertebrates mentioned are especially sea cucumbers. Sea cucumbers are echinoderms, members of the group Echinodermata, which also includes starfish and sea urchins. The sea cucumber as it is scientifically known has an elongated tubular body that is elastic and lacks a bony skeleton. They have the following taxonomic positions. Background technique [0002] The sea cucumber has the following taxonomic position: [0003] Subkingdom: Metazoa [0004] Phylum: Echinodermata [0005] Subphylum: Migratory Echinodermata...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C07K14/435A61K35/12A61K35/32A61K35/48A61K35/56C09B1/00
CPCC09B61/00
Inventor 乌沙·戈斯瓦米阿努陶什·甘古利
Owner 科学和工业研究委员会
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