Probiotic feed for aquaculture
An aquaculture, aquatic animal technology, applied in the direction of animal feed, animal feed, application, etc., can solve the problem of not making any progress
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[0048] Materials and methods
[0049] Microorganisms and Growth Media
[0050] Vibrio SY9 (BCCM accession number MUCL 54982) was previously isolated from the gastrointestinal tract of South African abalone (Macey & Coyne, 2005). A genetically modified Vibrio SY9 strain, named K811 strain, was used in this study to allow quantification of this bacterium in artificial feed formulations. Vibrio K811 (V. midae K811) is resistant to streptomycin and contains the bioluminescent plasmid pKluxCat. This plasmid is a derivative of pAKlux2 containing the lux operator (luxCDABE) from Photorhabdus luminescens (Karsi and Lawrence 2007). pAKlux2 was modified with the addition of the Tn9 chloramphenicol acetyltransferase (cat) gene to enable antibiotic selection of transzygotes (unpublished data). Vibrio K811 in tryptone soybean broth (30g / L NaCl, 17g / L tryptone, 6g / L peptone, 2.5g / L glucose, 2.5g / L K 2 HPO 4 , pH 7.8) and incubated in an orbital shaker at 150 rpm and 25°C.
[0051...
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