Application of a kind of eucalyptus eucalyptus essential oil and its compound bacterial agent in the preparation of insect repellent for echinoderms

A compound bacterial agent and insect repellant technology, which is applied in animal repellents, chemicals for biological control, applications, etc., can solve problems such as damage to the health of aquatic animals, unstable drug dosage, and hidden safety hazards for consumers. Achieve the effects of reducing bacterial infection, saving breeding costs, and using a wide range

Active Publication Date: 2020-05-15
SHANDONG LONGCHANG ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to simply pursue immediate results, traditional medicines will cause excessive heavy metals and unstable drug dosages, which will destroy the stability of water quality, damage the health of aquatic animals and reduce their immunity. In severe cases, it will directly lead to death. , ultimately reducing production efficiency and bringing safety hazards to consumers

Method used

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  • Application of a kind of eucalyptus eucalyptus essential oil and its compound bacterial agent in the preparation of insect repellent for echinoderms
  • Application of a kind of eucalyptus eucalyptus essential oil and its compound bacterial agent in the preparation of insect repellent for echinoderms
  • Application of a kind of eucalyptus eucalyptus essential oil and its compound bacterial agent in the preparation of insect repellent for echinoderms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] One, the preparation of Eucalyptus eucalyptus essential oil

[0030] The preparation process of Eucalyptus eucalyptus essential oil in the present invention is as figure 1 As shown, first put the leaves of Eucalyptus eucalyptus in Australia into the washing tower for cleaning, put them in the dryer for drying, and then pulverize them in the pulverizer and then enter the steam distillation kettle, condenser and oil-water stratifier for separation The oil phase and the water phase, the water phase enters the ether extractor to separate the waste water, the liquid containing ether enters the ether recovery tower to recover ether, and the recovered ether and oil phase liquid are added to the solid bed adsorption dryer to further remove the ether in the oil phase Finally, the oil phase liquid is added to a vacuum rectification tower for rectification to remove impurity oils to obtain eucalyptus multibract essential oil.

[0031] The essential oil of Eucalyptus multibract pr...

Embodiment 2

[0041] The multi-bract Eucalyptus eucalyptus essential oil compound bacterial agent prepared in Example 1 was used for the insect repellent experiment. The test site was selected in Linghai, Liaoning. Sea cucumbers were cultivated in 2000 cubic meters, each cement pond was 25 cubic meters, and the breeding specifications were 1000-1500 heads / catties . After festering and parasites were found on the surface of sea cucumbers, 1 kg of eucalyptus eucalyptus essential oil compound bacterial agent was used to mix 1 ton of feed, and the feeding was carried out according to the normal feeding management, and the feeding was continued for 4 days, 3 times a day. After 4 days, check the parasite situation of the sea cucumber again. Such as Figure 4 As shown, at the initial stage of feeding, the body surface of sea cucumbers festered, and ciliates were found in the wounds; after 4 days of feeding, it was found that the festered wounds of sea cucumbers were healed and there were no ciliat...

Embodiment 3

[0043] The eucalyptus eucalyptus essential oil prepared by the present invention was used for anthelmintic experiment, and the test site was selected as Dalian Sea Urchin Breeding Base. Ciliates were found in the body fluid of sea urchins, and 1 kg of essential oil of Eucalyptus eucalyptus was used to mix 1 ton of feed, and the sea urchins were continuously fed according to normal feeding management, 3 times a day. After 7 days, the situation of the parasites of the sea urchin was checked again, and it was found that there were no ciliates in the body fluid of the sea urchin, which indicated that Eucalyptus eucalyptus essential oil had an obvious deworming effect on the sea urchin.

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Abstract

The invention provides an application of Eucalyptus polybractea essential oil and its composite microbial agent in the preparation of a vermifuge for echinoderms. The Eucalyptus polybractea essentialoil composite microbial agent comprises Eucalyptus polybractea essential oil and Streptomyces griseus HL-50 with the bacterial content of 5 * 10<8> CFU / g or above, wherein the preservation number of the Streptomyces griseus HL-50 is CGMCC No.18424, and the most main effective component of the Eucalyptus polybractea essential oil is 1,8-cineole. The Eucalyptus polybractea essential oil composite microbial agent is simple to prepare and use, so effective repelling of various parasites infecting the echinoderms and bacterium inhibition can be achieved by only uniformly mixing an edible feed for the echinoderms with the composite microbial agent according to a ratio of 0.5-1.5 kg / t and continuously feeding the echinoderms with the obtained mixture for 4-7 d; and the composite microbial agent is nontoxic and harmless, is suitable for the echinoderms to use for a long term, can reduce the breeding cost, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to the application of essential oil of eucalyptus eucalyptus and its compound bacterial agent in preparing an insect repellant for echinoderms. Background technique [0002] Essential oils are volatile aromatic substances extracted from the flowers, leaves, stems, roots or fruits of plants through steam distillation, extrusion, cold soaking or solvent extraction, most of which have a lighter specific gravity than water. The relative density is generally in the range of 0.8 to 1.17. Essential oils are sensitive to heat and light, so they need to be stored in dark containers and in a cool place, and essential oils are now mostly used in skin care products and aromatherapy because of their special aroma and the characteristics of being soluble in organic solvents, but the function of plant essential oils Various, in addition to the traditional fragrance, soothing and i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N27/00A01N31/04A01N35/06A01N63/28A01N65/28C11B9/02A01P17/00
CPCA01N27/00A01N31/04A01N35/06A01N63/00C11B9/022C11B9/027Y02A50/30
Inventor 曹爱智籍立民娄倩倩
Owner SHANDONG LONGCHANG ANIMAL HEALTH PROD
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