Chinese herbal medicine immunopotentiator for special fur economic animals as well as preparation method and application of Chinese herbal medicine immunopotentiator

Abstract

The invention discloses a Chinese herbal medicine immunopotentiator for special fur economic animals as well as a preparation method and application of the Chinese herbal medicine immunopotentiator. Belongs to the technical field of traditional Chinese medicine preparations. The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 2-4 parts of echinacea purpurea, 2-4 parts of bighead atractylodes rhizome, 2-4 parts of Chinese yam, 1-2 parts of medlar and 1-2 parts of codonopsis pilosula. The raw material medicines are subjected to crushing, burdening, water boiling extraction, concentration and packaging to prepare the medicine. The Chinese herbal medicine immunopotentiator for the special fur economic animals has good effects of enhancing the disease resistance and disease prevention capability of racoon dog and improving the immunity.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine preparation technology, and more specifically to a special herbal immune enhancer for fur-bearing economic animals, its preparation method, and its application. Background Technology

[0002] In recent years, viral enteritis has frequently occurred and spread among special fur-bearing economic animals such as raccoon dogs, causing economic losses to farmers. To reduce the occurrence of these diseases and effectively activate the body's immune system, research on traditional Chinese medicine immune enhancers has received increasing attention. However, currently, there are no traditional Chinese medicine immune enhancers specifically formulated for special fur-bearing economic animals.

[0003] In summary, how to provide a traditional Chinese medicine immune enhancer for special fur-bearing economic animals is a problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0004] In view of this, the present invention provides a traditional Chinese medicine immune enhancer for special fur-bearing economic animals, its preparation method, and its application. The traditional Chinese medicine immune enhancer for special fur-bearing economic animals of the present invention can effectively improve the daily weight gain of raccoon dogs and the antibody level after vaccination.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A special herbal immune enhancer for fur-bearing economic animals, comprising the following components in parts by weight: 2-4 parts of Echinacea purpurea, 2-4 parts of Atractylodes macrocephala, 2-4 parts of Dioscorea opposita, 1-2 parts of Lycium barbarum, and 1-2 parts of Codonopsis pilosula.

[0007] The pharmacological effects of each component are as follows:

[0008] (1) Echinacea's effects: It has a bland and cool taste and enters the spleen, kidney, and stomach meridians. It contains polysaccharides, flavonoids, derivatives of caffeic acid, essential oils, polyacetylenes, alkylamines, and alkaloids. Polysaccharides can stimulate animal cells to fight against bacterial and viral infections, increase the phagocytic capacity of neutrophils and the number of peripheral lymphocytes, and stimulate the body's immune activity.

[0009] (2) Atractylodes macrocephala's effects: It is bitter and sweet in taste, warm in nature, and enters the spleen and stomach meridians. It contains a lot of nutrients such as polysaccharides and proteins, and has the effect of tonifying the middle and replenishing qi, which can improve the body's immunity.

[0010] (3) Efficacy of yam: It is sweet, warm, and neutral in nature, and enters the spleen and kidney meridians. It contains mucin, amylase, saponins, free amino acids, polyphenol oxidase, and other substances, which have the effects of nourishing and strengthening the body. It can increase the number of T lymphocytes in the body and enhance the body's immune function.

[0011] (4) Effects of wolfberry: It is sweet and neutral in nature, and enters the liver, kidney, and lung meridians. It contains wolfberry polysaccharides, monosaccharides, carotene, protein and polypeptides, vitamin B1, vitamin B2, 18 kinds of amino acids, and trace elements. It has the effects of nourishing the liver and kidneys, moistening the lungs and relieving cough.

[0012] (5) Codonopsis pilosula's effects: It is sweet and neutral in nature, and enters the liver, kidney, and lung meridians. It contains sterols, codonopsis glycosides, codonopsis polysaccharides, and 17 kinds of amino acids, as well as 11 kinds of trace elements such as iron, zinc, and copper. It can increase the phagocytic function of peritoneal macrophages, improve the lymphocyte transformation rate and E-roselet formation rate, and also increase animal weight, as well as have effects such as hypoxia tolerance and radiation resistance.

[0013] The formulation is based on the following:

[0014] This formula is based on the principle of strengthening the body's resistance to disease and eliminating pathogens, thereby enhancing the body's ability to fight and prevent disease and improving immunity. In this formula, echinacea, atractylodes macrocephala, and yam help the body fight bacteria and viruses, nourish the liver and kidneys, replenish essence and blood, and promote the production of qi and body fluids; while wolfberry and codonopsis clear heat and detoxify, cool the blood and stop bleeding, have antibacterial and anti-inflammatory effects, and regulate immune function. The combined effects of these herbs work synergistically to strengthen the body's resistance to disease, replenish qi and blood, promote the production of body fluids and blood, and enhance the body's immunity and disease resistance.

[0015] Furthermore, a special herbal immune enhancer for fur-bearing economic animals comprises the following components in parts by weight: 3 parts echinacea, 3 parts atractylodes macrocephala, 2 parts dioscorea opposita, 1 part lycium barbarum, and 1 part codonopsis pilosula.

[0016] Furthermore, the special fur-bearing economic animal is the raccoon dog.

[0017] The above-mentioned method for preparing the herbal immune enhancer for special fur-bearing economic animals involves weighing the raw materials and then sequentially pulverizing, soaking, decocting, concentrating, and sterilizing them.

[0018] Furthermore, the following steps are included:

[0019] (1) Weigh the raw materials, crush and sieve them for later use;

[0020] (2) Mix the coarse powder of four Chinese herbs, namely Atractylodes macrocephala, Dioscorea opposita, Lycium barbarum and Codonopsis pilosula, soak them in water, and boil them at 80℃~100℃ for 30 minutes.

[0021] (3) Add echinacea and simmer at 80℃~100℃ for another 10 minutes;

[0022] (4) Filter; add water and boil twice, 20 minutes each time;

[0023] (5) Combine the three filtrates, concentrate them, add sodium benzoate, and sterilize to obtain the finished product.

[0024] Furthermore, the sieving is performed through a 24-mesh sieve.

[0025] Furthermore, in step (2), the material-to-liquid ratio is (10-15):70g / mL, and the soaking time is 1 hour.

[0026] In the final product, 1 mL of the drug solution contains 1 g of the raw drug.

[0027] The application of the aforementioned herbal immune enhancers for special fur-bearing economic animals in promoting the growth performance of raccoon dogs.

[0028] Furthermore, the growth-promoting properties of raccoon dogs include increasing their appetite and growth rate.

[0029] The application of the above-mentioned special fur-bearing economic animal herbal immune enhancers in the preparation of antiviral enteritis drugs.

[0030] As can be seen from the above technical solution, compared with the prior art, the beneficial effects achieved by the present invention are as follows: the special herbal immune enhancer for fur-bearing economic animals of the present invention is suitable for the low immunity of the body caused by pathogenic microorganisms, improper use of drugs, nutritional deficiencies and stress, etc., and can enhance the body's immunity and disease resistance, with good effects. Detailed Implementation

[0031] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0032] The reagents required for this invention are conventional experimental reagents, purchased from commercially available channels; the experimental methods not mentioned are conventional experimental methods, and will not be described in detail here.

[0033] Example 1

[0034] A special herbal immune enhancer for fur-bearing economic animals, comprising the following components: 300g of Echinacea purpurea, 300g of Atractylodes macrocephala, 200g of Dioscorea opposita, 100g of Lycium barbarum, and 100g of Codonopsis pilosula.

[0035] The preparation method is as follows:

[0036] (1) Weigh the raw materials according to the above weight proportions.

[0037] (2) Crush the above raw materials into coarse Chinese medicine powder (all passed through No. 2 sieve, 24 mesh).

[0038] (3) Mix the coarse powder of four Chinese herbs: Atractylodes macrocephala, Dioscorea opposita, Lycium barbarum and Codonopsis pilosula, add 7000mL of water and soak for 1 hour. After boiling the water, decoct at 80℃~100℃ for 30 minutes, then add Echinacea purpurea and decoct at 80℃~100℃ for another 10 minutes. Filter the mixture. Add 5000mL of water each time and decoct twice, each time for 20 minutes.

[0039] (4) Filter, combine the three filtrates, concentrate to about 900 mL, add 0.05% sodium benzoate as a preservative, add water to 1000 mL, stir well, filter, fill, sterilize, and the special herbal immune enhancer for fur-producing economic animals of the present invention is thus prepared.

[0040] Example 2

[0041] A special herbal immune enhancer for fur-bearing economic animals, comprising the following components: 200g of Echinacea purpurea, 200g of Atractylodes macrocephala, 300g of Dioscorea opposita, 200g of Lycium barbarum, and 200g of Codonopsis pilosula.

[0042] The preparation method is the same as in Example 1.

[0043] Example 3

[0044] A special herbal immune enhancer for fur-bearing economic animals, comprising the following components: 400g of Echinacea purpurea, 400g of Atractylodes macrocephala, 400g of Dioscorea opposita, 150g of Lycium barbarum, and 150g of Codonopsis pilosula.

[0045] The preparation method is the same as in Example 1.

[0046] Experiment 1

[0047] Effects on growth performance and antibody titer of raccoon dogs

[0048] The herbal immune enhancer used in this experiment is the herbal immune enhancer prepared in Example 1.

[0049] Sixty raccoon dogs of uniform weight, raised to 40 days of age under normal conditions, were randomly divided into four groups: a high-dose group of traditional Chinese medicine (TCM) immune enhancers, a medium-dose group of TCM immune enhancers, a low-dose group of TCM immune enhancers, and a control group, with 15 dogs in each group. The raccoon dogs were immunized with raccoon viral enteritis vaccine at 45 days and 65 days of age, respectively. TCM immune enhancers were administered before and after both immunizations. Specifically, the TCM immune enhancers were added to the raccoon dogs' drinking water at the dosages shown in Table 1 5 days before and after immunization, and the dogs had free access to the water for 10 consecutive days. Grouping is shown in Table 1.

[0050] Table 1 Grouping

[0051]

[0052] Note: The table shows the volume percentage of the herbal immune enhancer. For example, 0.5% means that if the total volume is 100, the volume of the herbal immune enhancer is 0.5 and the volume of drinking water is 99.5.

[0053] Raccoon dogs were vaccinated against viral enteritis at 65 days of age, and serum samples were collected at 12, 24, 36, 48, and 60 days post-vaccination. The titer of hemagglutination inhibition antibodies against viral enteritis was determined. The number of morbid and fatal cases in each group was recorded during the trial. At 125 days of age, the dogs were weighed on an empty stomach between 7 and 8 am, and their weight was recorded. No other medications were administered to any group during the trial. The results are as follows:

[0054] ① Clinical manifestations of raccoon dogs in each group

[0055] The medium-dose group started on day 8 of the experiment (day 1 is defined as the first day of administration of the herbal immune enhancer), and the high-dose group started on day 5 of the experiment. The raccoons in both groups were more energetic, more active, had a better appetite, and had shinier fur. There was no significant difference in the low-dose group before and after the experiment.

[0056] On day 30 of the experiment, three animals in the control group developed the disease, with no deaths. No animals in the high-dose, medium-dose, or low-dose groups developed the disease or died. No further cases or deaths were observed in any of the groups until the end of the experiment.

[0057] ② Effects on the growth performance of raccoon dogs

[0058] The specific results are shown in Table 2.

[0059] Table 2 Growth performance of raccoon dogs

[0060]

[0061] Note: Identical lowercase letters in the same column indicate no significant difference; different lowercase letters in the same column indicate a significant difference; different uppercase letters indicate a highly significant difference. Initial weight was measured at 40 days of age, and final weight was measured at 125 days of age.

[0062] As shown in Table 2, during the experiment, the daily weight gain per animal in the low-, medium-, and high-dose groups and the control group was approximately 27.41g, 30.00g, 33.06g, and 23.06g, respectively. Throughout the experiment, the daily weight gain of the herbal immune enhancer group of this invention increased compared to the control group, indicating a better effect.

[0063] The herbal immune enhancer of this invention can significantly increase the daily weight gain of raccoon dogs and help improve their appetite and growth rate.

[0064] ③ Effects on antibody levels in raccoon dogs with viral enteritis

[0065] Following a second immunization against viral enteritis at 65 days of age, raccoon dogs had their serum collected at 12, 24, 36, 48, and 60 days after immunization to determine the titer of hemagglutination inhibition antibodies against viral enteritis. The results are shown in Table 3.

[0066] Table 3. Results of hemagglutination inhibition antibody titers

[0067]

[0068] Table 3 shows that the HI antibody level in raccoon dogs gradually increased 24 days after the second viral enteritis vaccine immunization, reaching a peak between 36 and 48 days. Experiments indicate that the antibody levels produced by drinking the herbal immune enhancer of this invention were higher than those in the control group, with the high-dose group showing the best results. At 48 days post-immunization, the high-dose group showed an average HI antibody titer of 1:128 in raccoon dogs, while the control group showed an average HI antibody titer of 1:44 between 24 and 48 days post-immunization.

[0069] Various experiments have shown that the herbal immune enhancer for special fur-bearing economic animals of this invention has significant improvements in various indicators such as the growth and development of raccoon dogs and the antibody titer for viral enteritis.

[0070] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0071] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A traditional Chinese medicine immune enhancer for special fur-bearing economic animals, characterized in that, The components include the following parts by weight: 2-4 parts echinacea, 2-4 parts atractylodes macrocephala, 2-4 parts dioscorea opposita, 1-2 parts lycium barbarum, and 1-2 parts codonopsis pilosula.

2. The herbal immune enhancer for special fur-bearing economic animals as described in claim 1, characterized in that, The components include the following parts by weight: 3 parts echinacea, 3 parts atractylodes macrocephala, 2 parts dioscorea opposita, 1 part lycium barbarum, and 1 part codonopsis pilosula.

3. The herbal immune enhancer for special fur-bearing economic animals as described in claim 1 or 2, characterized in that, The special fur-bearing economic animal mentioned is the raccoon dog.

4. The method for preparing the herbal immune enhancer for special fur-bearing economic animals as described in claim 1 or 2, characterized in that, After weighing the raw materials, they are successively crushed, soaked, boiled, concentrated, and sterilized.

5. The method as described in claim 4, characterized in that, Includes the following steps: (1) Weigh the raw materials, crush and sieve them for later use; (2) Mix the coarse powder of four Chinese herbs, namely Atractylodes macrocephala, Dioscorea opposita, Lycium barbarum and Codonopsis pilosula, soak them in water, and boil them at 80℃~100℃ for 30 minutes. (3) Add echinacea and simmer at 80℃~100℃ for another 10 minutes; (4) Filter; add water and boil twice, 20 minutes each time; (5) Combine the three filtrates, concentrate them, add sodium benzoate, and sterilize to obtain the finished product.

6. The method as described in claim 5, characterized in that, The sieving process involves passing the material through a 24-mesh sieve.

7. The method as described in claim 5, characterized in that, In step (2), the material-to-liquid ratio is (10-15):70g / mL, and the soaking time is 1 hour.

8. The application of the herbal immune enhancer for special fur-bearing economic animals as described in claim 1 or 2 in promoting the growth performance of raccoon dogs.

9. The application as described in claim 8, characterized in that, The growth-promoting properties of raccoon dogs are described as increasing their appetite and growth rate.

10. The use of the herbal immune enhancer for special fur-bearing economic animals as described in claim 1 or 2 in the preparation of antiviral enteritis drugs.

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