Reagent kit capable of simultaneously detecting virulent viruses and bacteria and detecting method

Abstract

The invention provides a reagent kit capable of simultaneously detecting virulent viruses and bacteria and a detecting method. The reagent kit is mainly in accordance with 4 pathogenic microorganismsof ebola viruses, Sinkiang hemorrhagic fever viruses, Brucella and anthrax bacillus. The reagent kit comprises a specific primer probe combination for detecting the ebola viruses, the Sinkiang hemorrhagic fever viruses, the Brucella and the anthrax bacillus, a micro-fluidic solid-phase PCR chip on which a specificity probe is fixed, and an RNase-resistant high-stability positive quality control product consisting of virus-like particles containing viral nucleic acid and thalli containing plasmid carrying the specificity nucleic acid. According to the reagent kit, simultaneous detection of theebola viruses, the Sinkiang hemorrhagic fever viruses, the Brucella and the anthrax bacillus can be realized, and the reagent kit has the advantages of being high in detection flux, high in sensitivity, high in specificity, good in repeatability, short in detection time, low in detection cost, low in operation technique requirements, not liable to pollute and the like, and has great application prospects in the field of quick simultaneous detection of various pathogenic microorganisms.

Description

technical field

[0001] The invention relates to the technical field of virus detection and molecular biology, in particular to a kit and a detection method for simultaneously detecting Ebola virus, Xinjiang hemorrhagic fever virus, Brucella and Bacillus anthracis. Background technique

[0002] Vigorous pathogenic microorganisms can be divided into viruses, bacteria, actinomycetes, fungi, chlamydia, rickettsia and spirochetes according to their species sources, among which viruses and bacteria are the main ones: viruses such as Ebola virus , Xinjiang hemorrhagic fever virus (Crimea-Congo hemorrhagic fever virus) and Zika virus, etc.; bacteria such as Bacillus anthracis, Brucella, Burkholderia malliensis and Francisella tularensis. According to the research data of the World Health Organization (WHO), infectious diseases caused by potent pathogenic microorganisms are an important part of human morbidity and mortality, and are the main factors that threaten human health and cau...

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