Fulminating-infectious-disease pathogen detecting primer pair and kit

A pathogen detection and infectious disease technology, applied in the field of severe infectious disease pathogen detection, can solve the problems of inconvenient to carry large instruments, unfavorable detection, cumbersome operation of fluorescent quantitative PCR technology, etc., to shorten the detection time, reduce the sample size, and improve sensitivity and safety effects
CN105483293AActive Publication Date: 2016-04-13INST OF PLA FOR DISEASE CONTROL & PREVENTION
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF PLA FOR DISEASE CONTROL & PREVENTION
Publication Date
2016-04-13

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Abstract

The invention discloses a fulminating-infectious-disease pathogen detecting primer pair and a kit. The primer pair comprises at least a pair of RT-LAMP primers of Ebola viruses, Lassa fever viruses, Marburg viruses, rift valley fever viruses, yellow fever viruses and Chikungunya fever viruses. By means of the primer system, the amplification reaction background is reduced, and sensitivity and specificity are quite good. The kit formed by the primer pair further comprises detecting liquid and a micro-fluidic chip; as an independent RT-PCR secondary amplification step of a detecting liquid system is omitted, detecting time is shortened; as the denaturation process and the renaturation process of nucleic acid do not exist, the polluted chance of RNA enzymes and the polluted chance of amplification nucleic acid are reduced, and the sensibility and the safety of detection are improved. By means of the constant-temperature sealed environment provided by a micro-fluidic chip system, rapid and constant-temperature amplification and automation result distinguishing of a nucleic acid extracting template are finally achieved, the requirement for test hardware is reduced, the use level of a reaction reagent is reduced, detection cost is reduced, and the result can be directly determined through color changes.
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Description

technical field

[0001] The invention relates to the technical field of detecting pathogens of severe infectious diseases, in particular to a primer set and a kit for detecting pathogens of severe infectious diseases. Background technique

[0002] In 2014, the worst Ebola epidemic in history broke out in West Africa, and it went out of Africa for the first time and spread to developed countries such as Europe and the United States, killing more than 11,000 people. The friendship between China and Africa has a long history, and the economic, trade and cultural exchanges are frequent and close. Therefore, the risk of Ebola epidemic importation is very high. After the outbreak, the Chinese government not only immediately provided a large amount of materials and technical assistance to African countries, but also dispatched medical and health experts to form a detection team and a medical team to help the local fight against the Ebola epidemic.

[0003] Lassa fever (Lassafever) ...

Claims

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