Galactosylated chitosan scaffold material for bioartificial liver and preparation method thereof

A technology for converting chitosan and a scaffold material, which is applied in the field of bioartificial liver galactosylated chitosan scaffold material and its preparation, and achieves the effects of low cytotoxicity, improved compressive strength and slowed down degradation rate.

Inactive Publication Date: 2011-12-14
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the current situation and shortcomings of the bioartificial liver scaffold materials in the prior art, the purpose of the present invention is to provide a structure with high porosity, a size s...

Method used

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  • Galactosylated chitosan scaffold material for bioartificial liver and preparation method thereof
  • Galactosylated chitosan scaffold material for bioartificial liver and preparation method thereof
  • Galactosylated chitosan scaffold material for bioartificial liver and preparation method thereof

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Embodiment 1

[0033] 3.24g chitosan is dissolved in the tetramethylethylenediamine / hydrochloric acid buffer solution of 240ml pH4.5, 7.16g lactobionic acid is dissolved in the tetramethylethylenediamine / hydrochloric acid buffer solution of 80ml pH4.5, two solutions combine Add 2.5ml of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride / N-hydroxysuccinic acid mixed solution (the molar ratio of both is 1:1) active intermediate, Activation reaction at about 25°C for 72 hours to prepare galactosylated chitosan, after the reaction, use 0.1M / L NaOH solution to adjust to near neutrality (pH about 7.0), repeated filtration and dialysis, and freeze-drying to obtain purified galactose Chitosan;

[0034] Galactosylated chitosan and oxidized sodium alginate were formulated into 2.5% solutions respectively, and the two solutions were mixed according to the dry weight ratio of galactosylated chitosan and oxidized sodium alginate at a ratio of 2:1, and stirred at 25°C for reaction About 10 minu...

Embodiment 2

[0037] 1.62g chitosan is dissolved in the tetramethylethylenediamine / hydrochloric acid buffer solution of 120ml pH4.7, 3.58g lactobionic acid is dissolved in the tetramethylethylenediamine / hydrochloric acid buffer solution of 40ml pH4.7, two solutions combine Add 1.0ml of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride / N-hydroxysuccinic acid mixed solution (the molar ratio of the two is 1:2) active intermediate, Activation reaction at about 20°C for 96 hours to prepare galactosylated chitosan, after the reaction, use 0.15M / L ammonia solution to adjust to near neutrality (pH about 7.2), repeat filtration and dialysis, and freeze-dry to obtain purified galactose Chitosan;

[0038] Prepare 2.5% solutions of galactosylated chitosan and oxidized sodium alginate respectively, mix the two solutions according to the dry weight ratio of galactosylated chitosan and oxidized sodium alginate at a ratio of 3:1, and stir at about 20°C React for about 20 minutes, pour into the ...

Embodiment 3

[0041] 1.62g chitosan is dissolved in the tetramethylethylenediamine / hydrochloric acid buffer solution of 120ml pH4.8, 3.58g lactobionic acid is dissolved in the tetramethylethylenediamine / hydrochloric acid buffer solution of 40ml pH4.8, two solutions combine Add 1.2ml of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride / N-hydroxysuccinic acid mixed solution (the molar ratio of both is 2:1) active intermediate, The galactosylated chitosan was prepared by activating the reaction at about 30°C for about 60 hours. After the reaction, it was adjusted to near neutrality (pH about 7.1) with 0.15M / L KOH solution, filtered and dialyzed repeatedly, and lyophilized to obtain purified semi-galactosyl chitosan. Lactosylated chitosan;

[0042] Prepare 2.5% solutions of galactosylated chitosan and oxidized sodium alginate respectively, mix the two solutions according to the dry weight ratio of galactosylated chitosan and oxidized sodium alginate at a ratio of 4:1, and stir at abo...

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Abstract

The invention discloses a galactosylated chitosan scaffold material for a bioartificial liver, which is a galactosylated chitosan biological material with a three-dimensional porous structure which is cross-linked by an oxidized sodium alginate biological crosslinking agent. Its main preparation process: chitosan and lactobionic acid are dissolved in tetramethylethylenediamine/hydrochloric acid mixed solution in an equimolar ratio, and active intermediates are added to activate the reaction to prepare galactosylated chitosan; galactosylated chitosan is dissolved in Acetic acid solution and oxidized sodium alginate are dissolved in ultrapure water, and the two solutions are mixed in a certain proportion to carry out cross-linking reaction. After the reaction is completed, pour it into a mold for molding, freeze-dry to obtain a scaffold material, and then immerse it in sodium hydroxide- The residual acetic acid was removed by ethanol aqueous solution, and then washed by ethanol aqueous solution and freeze-dried twice to obtain the galactosylated chitosan scaffold material cross-linked by oxidized sodium alginate. The galactosylated chitosan scaffold material of the invention has a pore size suitable for the growth of liver cells and good biocompatibility.

Description

technical field [0001] The invention relates to a chitosan scaffold biomaterial technology, in particular to a galactosylated chitosan scaffold material for a bioartificial liver and a preparation method thereof. technical background [0002] The liver is an important detoxification organ of the body. No matter what the reason is, once a large amount of necrosis of liver cells and liver failure occur, it will inevitably lead to metabolic disorders and a large accumulation of toxic substances. Cell necrosis affects the regeneration of remaining liver cells, thereby aggravating liver failure and forming a vicious circle. The starting point of artificial liver research is to use artificial liver to detoxify, complete biosynthesis, biotransformation and other liver functions, break the above vicious cycle, stabilize the internal environment of patients with liver failure, and regenerate liver cells with strong regenerative ability or further improve liver function. Create condi...

Claims

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Application Information

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IPC IPC(8): A61L27/20
Inventor 余喜讯顾志鹏徐源廷张旭彭红陈峰万昌秀张小华
Owner SICHUAN UNIV
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