Liver cancer-targeted multi-walled carbon nanotube drug-loaded composite material and preparation method thereof

A technology of multi-walled carbon nanotubes and composite materials, applied in the field of multi-walled carbon nanotube drug-loaded composite materials and their preparation, can solve the problems of difficulty in dispersion and easy accumulation of carbon nanotubes, and achieves high release rate and mild experimental conditions. , the effect of simple operation

Inactive Publication Date: 2014-08-20
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the characteristics of carbon nanotubes that are easy to accumulate and difficult to disperse, the research on carbon nanotubes needs to be further explored.

Method used

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  • Liver cancer-targeted multi-walled carbon nanotube drug-loaded composite material and preparation method thereof
  • Liver cancer-targeted multi-walled carbon nanotube drug-loaded composite material and preparation method thereof
  • Liver cancer-targeted multi-walled carbon nanotube drug-loaded composite material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Dissolve 5 mg of carbon nanotube composite material in 5 ml of water, add 5 mL of an aqueous solution containing DOX (5 mg) dropwise under stirring, adjust the pH to 9.0 with 0.1 M sodium hydroxide solution, and Under stirring for 4h. Finally, put the doxorubicin-containing carbon nanotube solution into a dialysis bag (MW=8,000-14,000), remove unconnected doxorubicin by dialysis, and dialyze three times with PBS buffer, 2 L each time. Finally, the product was freeze-dried to obtain CNT / DOX.

[0041](2) Weigh 2 mg doxorubicin, prepare respectively 0.002M, 0.004M, 0.008M, 0.016M, 0.032M PBS solution (pH=7.4) and acetic acid solution (pH=5.8), and carry out ultraviolet light on the doxorubicin solution For detection, the absorption wavelength was set to 481nm, and the data were collected and fitted to a standard curve of doxorubicin under two pH conditions.

[0042] (3) Weigh 2mg of the drug-loaded complex in 2ml of phosphate buffer (pH=7.4), dissolve it fully, put i...

Embodiment 2

[0045] (1) Dissolve 10.3 mg of carbon nanotube composite material in 10.3 ml of water, add 13 mL of an aqueous solution containing DOX (6 mg) dropwise while stirring, and adjust the pH to 9.0 with 0.1 M sodium hydroxide solution. Stir at room temperature for 4h. Finally, put the doxorubicin-containing carbon nanotube solution into a dialysis bag (MW=8,000-14,000), remove unconnected doxorubicin by dialysis, and dialyze three times with PBS buffer, 2 L each time. Finally, the product was freeze-dried to obtain CNT / DOX.

[0046] (2) Weigh 2 mg doxorubicin, prepare 0.0025M, 0.005M, 0.01M, 0.02M, 0.04M PBS solution (pH=7.4) and acetic acid solution (pH=5.8) respectively, and carry out ultraviolet light on the doxorubicin solution For detection, the absorption wavelength was set to 481nm, and the data were collected and fitted to a standard curve of doxorubicin under two pH conditions.

[0047] (3) Weigh 4.5mg of the drug-loaded complex in 4.5ml of phosphate buffer (pH=7.4), full...

Embodiment 3

[0050] (1) Dissolve 10 mg of carbon nanotube composite material in 10 ml of water, add 5 mL of an aqueous solution containing DOX (10 mg) dropwise while stirring, and adjust pH=5.5, 6.5, 7.5, 8.5 and 9.5, stirred at room temperature for 4h. Finally, the carbon nanotube solutions containing doxorubicin at different pH values ​​were put into dialysis bags (MW=8,000-14,000), and the unattached doxorubicin was removed by dialysis, and dialyzed three times with PBS buffer, each times 2L. Finally, the product was freeze-dried to obtain CNT / DOX.

[0051] (2) Weigh 2 mg doxorubicin, prepare respectively 0.001M, 0.002M, 0.004M, 0.012M, 0.036M PBS solution (pH=7.4) and acetic acid solution (pH=5.8), and carry out ultraviolet light on the doxorubicin solution For detection, the absorption wavelength was set to 481nm, and the data were collected and fitted to a standard curve of doxorubicin under two pH conditions.

[0052] (3) Weigh 0.5 mg of drug-loaded complexes in 1 ml of phosphate...

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Abstract

The invention relates to a liver cancer-targeted multi-walled carbon nanotube drug-loaded composite material and a preparation method thereof. The composite material is a compound of doxorubicin (doxorubicin) and a lactobionic acid modified multi-walled carbon nanotube CNT-PEI-FI-PEG-LA. The preparation method comprises the following steps: synthesizing the multi-walled carbon nanotube composite material CNT-PEI-FI-PEG-LA; formulating doxorubicin standard curves in two types of pH value environments; and dissolving the multi-walled carbon nanotube composite material in water, adding a doxorubicin hydrochloride aqueous solution, adjusting the pH value to neutrality and alkalinity, stirring for 4 hours, dialyzing, and carrying out freeze drying, thus obtaining the liver cancer-targeted multi-walled carbon nanotube drug-loaded composite material. The liver cancer-targeted multi-walled carbon nanotube drug-loaded composite material has the characteristics of pH sensitive release and liver cancer cell targeting, can be used for anti-tumor research and has very high practical value.

Description

technical field [0001] The invention belongs to the field of liver cancer targeting nanomaterials and preparation thereof, in particular to a multi-walled carbon nanotube drug-loaded composite material targeting liver cancer and a preparation method thereof. Background technique [0002] Carbon nanotubes can be broadly classified into single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs). They are graphite tubular crystals formed by curling single-layer or multi-layer graphite according to a certain helical angle. According to the configuration, they can also be divided into zig-zag, amchair and chiral. The top of carbon nanotubes is mostly composed of pentagonal or heptagonal carbon rings. Carbon nanotubes have various shapes, such as cylindrical, ring, coil and bamboo. Due to its unique properties, carbon nanotubes have shown good application prospects in biology, chemistry, and materials. However, because carbon nanotubes are easy to accumul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K47/04A61K47/26A61K31/704A61P35/00
Inventor 朱利民陶磊
Owner DONGHUA UNIV
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