Preparation method of hepatoma carcinoma cell targeted molybdenum disulfide drug-loaded nano tablets

A technology of molybdenum disulfide and liver cancer cells, which can be applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, etc. Large damage and other problems, to achieve high drug loading efficiency, adjustable particle size, and strong active targeting

Inactive Publication Date: 2015-07-29
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Surgical treatment is usually unable to cure advanced patients with metastatic tumors, and the secondary damage to patients caused by radiotherapy and chemotherapy is sometimes greater than the cancer itself
Most of the existing anti-tumor drugs are not only highly toxic but also lack targeting. They kill a large number of normal cells while killing liver tumor cells
In addition, due to the unique biological characteristics of liver cancer cells, it is difficult for anti-tumor drugs to enter the cells inside the tumor, making it difficult to achieve an effective concentration at the target site
Therefore, relying solely on the above methods for tumor treatment will have considerable limitations.

Method used

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  • Preparation method of hepatoma carcinoma cell targeted molybdenum disulfide drug-loaded nano tablets
  • Preparation method of hepatoma carcinoma cell targeted molybdenum disulfide drug-loaded nano tablets
  • Preparation method of hepatoma carcinoma cell targeted molybdenum disulfide drug-loaded nano tablets

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Embodiment 1

[0027] A preparation method of molybdenum disulfide drug-loaded nanosheets with liver cancer cell targeting, specifically as follows:

[0028] Step 1: Prepare 40mL of ammonium tetrathiomolybdate aqueous solution with a concentration of 5mg / mL, stir until transparent, add to a 50mL reaction kettle, heat to 160°C, and keep for 10h; after cooling to room temperature, centrifuge to collect, use deionized Wash with water, repeat the centrifugation and washing operations, and collect Figure 1a with 1b Molybdenum disulfide nanosheets in

[0029] Step 2: Prepare 20 mL of gelatin solution with a concentration of 50 mg / mL, add 0.15 g of molybdenum disulfide nanosheets prepared in step 1 into the prepared gelatin solution, stir for 12 hours, and allow it to fully react to obtain gelatinized molybdenum disulfide nanosheets piece;

[0030] Step 3: Weigh 30 mg of lactobionic acid, 15 mg of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 7.5 mg of N-hydroxysuccinimide int...

Embodiment 2

[0035] A preparation method of molybdenum disulfide drug-loaded nanosheets with liver cancer cell targeting, specifically as follows:

[0036]Step 1: Prepare 50mL of ammonium tetrathiomolybdate aqueous solution with a concentration of 30mg / mL, stir until transparent, add to a 50mL reaction kettle, heat to 200°C, keep for 12h; after cooling to room temperature, centrifuge to collect, use deionized Wash with water, repeat the centrifugation and washing operations, and collect the molybdenum disulfide nanosheets;

[0037] Step 2: Prepare 10 mL of gelatin solution with a concentration of 100 mg / mL, add 1 g of molybdenum disulfide nanosheets prepared in step 1 into the prepared gelatin solution, stir for 12 hours, and make it fully react to obtain gelatinized molybdenum disulfide nanosheets ;

[0038] Step 3: Weigh 40 mg of lactobionic acid, 20 mg of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 10 mg of N-hydroxysuccinimide into step 2 to prepare 0.2g of gelat...

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Abstract

The invention provides a preparation method of a hepatoma carcinoma cell targeted molybdenum disulfide drug-loaded nano tablets. The preparation method comprises following steps: step 1, molybdenum disulfide nano tablets are obtained via hydro-thermal synthesis; step 2, the molybdenum disulfide nano tablets are added into a gelatin solution, and gelatinized molybdenum disulfide nano tablets are obtained via sufficient reaction; step 3, lactobionic acid, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, and N-hydroxy succinimide are weighed and added into the gelatinized molybdenum disulfide nano tablets so as to obtain lactobionic acid modified molybdenum disulfide nano tablets; and step 4, a doxorubicin hydrochloride aqueous solution is prepared, and the lactobionic acid modified molybdenum disulfide nano tablets obtained via step 3 are added into the doxorubicin hydrochloride aqueous solution, an obtained mixture is stirred for 8 to 24h under vacuum conditions, and is subjected to centrifugalization and washing so as to obtain the hepatoma carcinoma cell targeted molybdenum disulfide drug-loaded nano tablets via collecting. The hepatoma carcinoma cell targeted molybdenum disulfide drug-loaded nano tablets can be used for photothermal therapy and CT imaging, and can be used for realizing combination of diagnosis and treatment of hepatoma carcinoma.

Description

technical field [0001] The invention discloses a preparation method of molybdenum disulfide drug-loaded nanosheets with liver cancer targeting. More specifically, it involves the preparation of molybdenum disulfide nanosheets by the hydrothermal method, and the loading of anticancer drugs such as doxorubicin after surface modification of lactobionic acid, so that it has liver cancer targeting, photothermal therapy, CT imaging and chemotherapy. It provides a new way for the diagnosis and treatment of liver cancer. Background technique [0002] The threat of malignant tumors to human beings has become increasingly prominent and has become an important cause of death. Among them, liver cancer is one of the most common malignant tumors in my country. Early diagnosis and effective treatment of tumors are extremely important to human health and sustainable development. In the past few decades, human beings have conducted in-depth exploration of tumor characteristics, which has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K49/04A61K31/704A61K47/04A61K47/26A61P35/00
Inventor 何创龙冯炜秦明常安妮陈良
Owner DONGHUA UNIV
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