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Measuring method of pectin content in tobaccos and tobacco products

A technology for tobacco products and determination methods, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of long processing time, affecting the accuracy and reproducibility of analysis results, and complicated operation, and achieves shortened operation and time. Application prospect, high sensitivity effect

Active Publication Date: 2014-01-22
HONGTA TOBACCO GRP
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  • Abstract
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  • Claims
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Problems solved by technology

The accuracy of the 3,5-dinitrosalicylic acid method is not enough, the flow analysis method, the stability of the instrument is not good, and the waste of reagents
This method needs to go through repeated reflux and filtration, the operation is cumbersome, and the amount of reagent used in the flow analyzer is large.
The tobacco industry adopts YC / T 346-2010 "Ion Chromatography for the Determination of Pectin in Tobacco and Tobacco Products". This method has high selectivity and sensitivity, but there is a problem: the pretreatment process is complicated and needs to be carried out many times. Filtration, washing, transfer and dilution of solution, long processing time
And due to the transmission of errors, there is a possibility of error amplification and fluctuations in test results, which will affect the accuracy and reproducibility of analysis results

Method used

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  • Measuring method of pectin content in tobaccos and tobacco products
  • Measuring method of pectin content in tobaccos and tobacco products
  • Measuring method of pectin content in tobaccos and tobacco products

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Experimental program
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Embodiment

[0066] Sample pretreatment

[0067] A． Tobacco samples were crushed with a pulverizer, passed through a 40-mesh sieve, and their moisture content was determined w , accurately weighed 0.5 g (accurate to 0.0001 g) of tobacco sample, placed in a 250 mL round bottom flask, accurately added 100 mL of hydrochloric acid-ethanol solution with a hydrogen ion concentration of 0.05 mol / L and an alcohol concentration of 80%, and the round Place the bottom flask on the electric heating mantle, connect the condenser, heat and reflux for 1 h, suction filter while it is hot, rinse with hot water three times, and dilute the filtrate to 200 mL to obtain solution a;

[0068] B. Transfer the residue of the above suction-filtered sample to a 200 mL ground-mouth Erlenmeyer flask, add 100 mL of acetic acid / sodium acetate buffer solution and 40 μL of pectinase solution, enzymatically hydrolyze in a water bath at 53 °C for 3 h, suction filter, and dilute the filtrate to 200 mL to obtain solutio...

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Abstract

The invention discloses a measuring method of pectin content in tobaccos and tobacco products, belonging to the technical field of tobacco analysis detection. The measuring method of the pectin content in tobaccos and tobacco products comprises the following steps: (1), adding acidic alcohol liquor into a tobacco sample, then, heating and refluxing, carrying out solid-liquid separation to obtain filtrate and filter residues, and making up to volume after cooling the filtrate to obtain liquor a; (2), adding acetic acid / sodium acetate buffer liquor and pectinase liquor into the filter residues, placing in a water bath for enzymolysis, carrying out suction filtration, and making up to volume to obtain liquor b; (3), getting a certain amount of liquor a, adding the acetic acid / sodium acetate buffer liquor and the pectinase liquor, placing in the water bath for enzymolysis, carrying out suction filtration, transferring the filtrate to a volumetric flask, adding a certain amount of liquor b, making up to volume to obtain to-be-measured liquor; and (4), enabling the to-be-measured liquor to pass through a 0.45-micron filter membrane and then carrying out ion chromatography analysis. The pectin content is calculated in terms of content of lactobionic acid. The measuring method disclosed by the invention is simple to operate, good in repeatability and high in recovery rate, and capable of satisfying batch measurement requirement of pectin content in the tobaccos and the tobacco products.

Description

technical field [0001] The invention relates to a method for measuring pectin content in tobacco and tobacco products, belonging to the technical field of tobacco analysis and detection. Background technique [0002] Pectin exists in the cell wall and inner layer of plants and is an important part of plant cells. Its basic structure is polygalacturonic acid formed by the polymerization of galacturonic acid with α-1,4 glycosidic bonds. Pectin in plants generally has three forms: protopectin, water-soluble pectin and pectin acid. Protopectin is polygalacturonic acid combined with soluble pectin acid and cell wall components such as cellulose, carboxyl A small part of it is methylated and insoluble in water; water-soluble pectin is polygalacturonic acid whose carboxyl groups have been methylated to varying degrees, and is soluble in water; pectin acid is polygalacturonic acid with completely free carboxyl groups acid, can interact with Ca in cell juice 2+ or Mg 2+ 、K + 、Na ...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/02
Inventor 汤建国柏婷伊奥尔乔丹娜向能军孟昭宇牟定荣
Owner HONGTA TOBACCO GRP
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