Measuring method of pectin content in tobaccos and tobacco products

A technology for tobacco products and determination methods, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of long processing time, affecting the accuracy and reproducibility of analysis results, and complicated operation, and achieves shortened operation and time. Application prospect, high sensitivity effect
CN103529147AActive Publication Date: 2014-01-22HONGTA TOBACCO GRP
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HONGTA TOBACCO GRP
Publication Date
2014-01-22

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Abstract

The invention discloses a measuring method of pectin content in tobaccos and tobacco products, belonging to the technical field of tobacco analysis detection. The measuring method of the pectin content in tobaccos and tobacco products comprises the following steps: (1), adding acidic alcohol liquor into a tobacco sample, then, heating and refluxing, carrying out solid-liquid separation to obtain filtrate and filter residues, and making up to volume after cooling the filtrate to obtain liquor a; (2), adding acetic acid / sodium acetate buffer liquor and pectinase liquor into the filter residues, placing in a water bath for enzymolysis, carrying out suction filtration, and making up to volume to obtain liquor b; (3), getting a certain amount of liquor a, adding the acetic acid / sodium acetate buffer liquor and the pectinase liquor, placing in the water bath for enzymolysis, carrying out suction filtration, transferring the filtrate to a volumetric flask, adding a certain amount of liquor b, making up to volume to obtain to-be-measured liquor; and (4), enabling the to-be-measured liquor to pass through a 0.45-micron filter membrane and then carrying out ion chromatography analysis. The pectin content is calculated in terms of content of lactobionic acid. The measuring method disclosed by the invention is simple to operate, good in repeatability and high in recovery rate, and capable of satisfying batch measurement requirement of pectin content in the tobaccos and the tobacco products.
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Description

technical field

[0001] The invention relates to a method for measuring pectin content in tobacco and tobacco products, belonging to the technical field of tobacco analysis and detection. Background technique

[0002] Pectin exists in the cell wall and inner layer of plants and is an important part of plant cells. Its basic structure is polygalacturonic acid formed by the polymerization of galacturonic acid with α-1,4 glycosidic bonds. Pectin in plants generally has three forms: protopectin, water-soluble pectin and pectin acid. Protopectin is polygalacturonic acid combined with soluble pectin acid and cell wall components such as cellulose, carboxyl A small part of it is methylated and insoluble in water; water-soluble pectin is polygalacturonic acid whose carboxyl groups have been methylated to varying degrees, and is soluble in water; pectin acid is polygalacturonic acid with completely free carboxyl groups acid, can interact with Ca in cell juice 2+ or Mg 2+ 、K + 、Na ...

Claims

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