Oxidized graphene medicine-carried composite material for liver cancer targeting and preparation method of composite material

A composite material and graphene technology, which can be used in drug combinations, pharmaceutical formulations, and non-active ingredients medical preparations, etc., can solve problems such as large lethality, and achieve the effects of high release rate, simple operation, and mild experimental conditions.

Inactive Publication Date: 2014-11-19
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, DOX will have greater lethality to normal cells in large doses. Therefore, in order to reduce the toxic and side effects of DOX on normal tissues of the human body, it is necessary to use drug carriers to load DOX and control its release.
[0006] A search of

Method used

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  • Oxidized graphene medicine-carried composite material for liver cancer targeting and preparation method of composite material
  • Oxidized graphene medicine-carried composite material for liver cancer targeting and preparation method of composite material
  • Oxidized graphene medicine-carried composite material for liver cancer targeting and preparation method of composite material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Dissolve 5mg of graphene oxide composite material in 5ml of water, add 5mL of DOX (5mg) aqueous solution dropwise while stirring, adjust the pH to 9.0 with 0.1M sodium hydroxide solution, at room temperature Under stirring for 4h. Finally, the graphene oxide solution containing doxorubicin was put into a dialysis bag (MW=8,000-14,000), and the unattached doxorubicin was removed by dialysis, and dialyzed three times with PBS buffer, 2 L each time. Finally, the product was freeze-dried to obtain GO / DOX.

[0043](2) Weigh 2 mg doxorubicin, prepare respectively 0.002M, 0.004M, 0.008M, 0.016M, 0.032M PBS solution (pH=7.4) and acetic acid solution (pH=5.8), and carry out ultraviolet light on the doxorubicin solution For detection, the absorption wavelength was set to 481nm, and the data were collected and fitted to a standard curve of doxorubicin under two pH conditions.

[0044] (3) Weigh 2mg of the drug-loaded complex in 2ml of phosphate buffer (pH=7.4), dissolve it f...

Embodiment 2

[0047] (1) Dissolve 8mg of graphene oxide composite material in 8ml of water, and add 13mL of DOX (6mg) aqueous solution dropwise while stirring, and adjust the pH to 9.0 with 0.1M sodium hydroxide solution. Under stirring for 4h. Finally, the graphene oxide solution containing doxorubicin was put into a dialysis bag (MW=8,000-14,000), and the unattached doxorubicin was removed by dialysis, and dialyzed three times with PBS buffer, 2 L each time. Finally, the product was freeze-dried to obtain GO / DOX.

[0048] (2) Weigh 3mg doxorubicin, prepare respectively 0.0025M, 0.005M, 0.01M, 0.02M, 0.04M PBS solution (pH=7.4) and acetic acid solution (pH=5.8), and carry out ultraviolet light on the doxorubicin solution For detection, the absorption wavelength was set to 481nm, and the data were collected and fitted to a standard curve of doxorubicin under two pH conditions.

[0049] (3) Weigh 4.5mg of the drug-loaded complex in 4.5ml of phosphate buffer (pH=7.4), fully dissolve it, put...

Embodiment 3

[0052] (1) Dissolve 10mg of graphene oxide composite material in 10ml of water, add 5mL of DOX (10mg) aqueous solution dropwise while stirring, and adjust pH=5.5, 6.5, 7.5, 8.5 and 9.5, stirred at room temperature for 4h. Finally, the graphene oxide solutions containing doxorubicin at different pH values ​​were put into dialysis bags (MW=8,000-14,000), and the unattached doxorubicin was removed by dialysis, and dialyzed three times with PBS buffer, each times 2L. Finally, the product was freeze-dried to obtain GO / DOX.

[0053] (2) Weigh 2 mg doxorubicin, prepare respectively 0.001M, 0.002M, 0.004M, 0.012M, 0.036M PBS solution (pH=7.4) and acetic acid solution (pH=5.8), and carry out ultraviolet light on the doxorubicin solution For detection, the absorption wavelength was set to 481nm, and the data were collected and fitted to a standard curve of doxorubicin under two pH conditions.

[0054] (3) Weigh 0.5 mg of drug-loaded complexes in 1 ml of phosphate buffer (pH=7.4), ful...

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Abstract

The invention relates to an oxidized graphene medicine-carried composite material for liver cancer targeting and a preparation method of the composite material. The obtained composite material is a compound formed by doxorubicin DOX and oxidized graphene GO-PEI-FI-PEG-LA which is modified by a lactobionic acid. The preparation method comprises the following steps: synthesizing the oxidized graphene composite material GO-PEI-FI-PEG-LA; preparing standard doxorubicin curves under the environment with two pH values; dissolving the oxidized graphene composite material in water, adding an aqueous solution of doxorubicin hydrochloride and regulating the pH value to obtain a neutral and alkaline value, stirring for 4 hours, dialyzing, freezing and drying so as to obtain the oxidized graphene medicine-carried composite material. The novel medicine-carried material provided by the invention has the characteristics of pH-sensitive releasing and liver cancer cell targeting, can be applied to anti-tumor researches and has a good practical value.

Description

technical field [0001] The invention belongs to the field of liver cancer targeting nanomaterials and preparation thereof, in particular to a liver cancer targeting graphene oxide drug-loaded composite material and a preparation method thereof. Background technique [0002] The toxic side effects and poor water solubility of anticancer drugs have always been the bottleneck problems in tumor chemotherapy, so the use of drug delivery systems has received extensive attention. With its unique size range (1-100nm), the nano-drug loading system can not only reduce the biological toxicity of drugs, effectively improve the solubility and stability of drugs, but also can and can be modified through the modification of drug carriers, the selection of carrier drugs and loading methods. It is designed to control the sustained release and directional delivery of drugs, so as to achieve the best therapeutic effect. [0003] As a new type of two-dimensional nanomaterial, graphene has uniq...

Claims

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Application Information

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IPC IPC(8): A61K9/00A61K31/704A61K47/04A61P35/00
Inventor 朱利民陶磊杨卉卉公晓曹欣乐
Owner DONGHUA UNIV
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