Preparation method of composite material containing lactobionic acid-modified graphene oxide

A composite material and graphene technology, which is applied in the field of preparation of graphene oxide composite materials, can solve the problems of increasing cytotoxicity, achieve mild experimental conditions, simple operation, and overcome the effect of easy agglomeration

Inactive Publication Date: 2014-11-26
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, too many surface amino groups also increase its cytotoxicity, thus limiting the application of PEI.

Method used

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  • Preparation method of composite material containing lactobionic acid-modified graphene oxide
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  • Preparation method of composite material containing lactobionic acid-modified graphene oxide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Dissolve GO (344.9mg) in 40mL DMSO, then add EDC (354.32mg) to activate and stir for 3h. While stirring, 10 mL of DMSO solution containing PEI (540.01 mg) was added dropwise, and the reaction was stirred ultrasonically at room temperature for 24 h. Finally, the reaction solution was put into a dialysis bag (MW=100,000), and unreacted impurities and by-products were removed by dialysis. Firstly, it was dialyzed twice with PBS buffer, 2 L each time, and then dialyzed seven times with distilled water. Finally, the product was freeze-dried to obtain GO-PEI.

[0034] (2) Weigh 100 mg LA, add 35 mg EDC and 21 mg NHS to phosphate buffer to activate for three hours, add 400 mg NH2-mPEG-COOH, stir for 24 hours, and finally put the reaction solution into a dialysis bag (MW=1,000), and use dialysis to remove the unreacted To remove impurities and by-products, dialyze twice with PBS buffer, 2L each time, and then dialyze seven times with distilled water. Finally the product i...

Embodiment 2

[0038] (1) Dissolve GO (300mg) in 30mL DMSO, then add EDC (304mg) to activate and stir for 3h. While stirring, 10 mL of DMSO solution containing PEI (452.3 mg) was added dropwise, and the reaction was stirred ultrasonically at room temperature for 24 h. Finally, the reaction solution was put into a dialysis bag (MW=100,000), and unreacted impurities and by-products were removed by dialysis. Firstly, it was dialyzed twice with PBS buffer, 2 L each time, and then dialyzed seven times with distilled water. Finally, the product was freeze-dried to obtain GO-PEI.

[0039] (2) Weigh 23 mg LA, add 8.01 mg EDC and 4.8 mg NHS to phosphate buffer to activate for three hours, add 100 mg NH2-mPEG-COOH, stir for 24 hours, and finally put the reaction solution into a dialysis bag (MW=1,000), and use dialysis to dissolve To remove unreacted impurities and by-products, dialyze twice with PBS buffer, 2 L each time, and then dialyze seven times with distilled water. Finally the product is fre...

Embodiment 3

[0043] (1) Dissolve GO (50mg) in 10mL DMSO, then add EDC (33mg) to activate and stir for 3h. With stirring, 10 mL of DMSO solution containing PEI (75 mg) was added dropwise, and the reaction was stirred ultrasonically at room temperature for 24 h. Finally, the reaction solution was put into a dialysis bag (MW=100,000), and unreacted impurities and by-products were removed by dialysis. Firstly, it was dialyzed twice with PBS buffer, 2 L each time, and then dialyzed seven times with distilled water. Finally, the product was freeze-dried to obtain GO-PEI.

[0044] (2) Weigh 44 mg LA, add 21 mg EDC and 11 mg NHS to phosphate buffer to activate for three hours, add 180 mg NH2-mPEG-COOH, stir for 24 hours, and finally put the reaction solution into a dialysis bag (MW=1,000), and use dialysis to remove the unreacted To remove impurities and by-products, dialyze twice with PBS buffer, 2L each time, and then dialyze seven times with distilled water. Finally the product is freeze-drie...

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Abstract

The invention relates to a preparation method of a composite material containing lactobionic acid-modified graphene oxide, and the method is as follows: chemically bonding graphene oxide (GO) and PEI (polyethyleneimine) in dimethyl sulfoxide by the effect of activator EDC (1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide), dialyzing, and freeze-drying for synthesis of GO-PEI; dissolving the LA in a phosphate buffer solution, adding EDC and NHS (N-Hydroxysuccinimide) for activation, adding NH2-mPEG-COOH, reacting for 12-24h at room temperature, dialyzing, and freeze-drying to obtain PEG-LA; dissolving the GO-PEI in water, adding a FITC (fluorescein isothiocyanate) solution; adding the EDC and the NHS into the PEG-LA for activation, adding the GO-PEI solution for reaction to obtain GO-PEI-FI-PEG-LA, adding triethylamine for mixing, adding acetic anhydride, reacting at room temperature, dialyzing, and freeze-drying to obtain the graphene oxide composite material. The preparation process of the preparation method is simple, the experimental conditions are normal temperature and pressure, and the preparation method is easy to operate.

Description

technical field [0001] The invention belongs to the field of preparation of graphene oxide composite materials, in particular to a method for preparing graphene oxide composite materials modified with lactobionic acid. Background technique [0002] As a new type of two-dimensional nanomaterial, graphene has become a research hotspot due to its unique and excellent electrical, optical, and mechanical properties, as well as its wide application prospects. [0003] Graphene derivatives that are widely used in the biomedical field are mainly functionalized graphene oxide (or graphene oxide). Graphene oxide is usually prepared from graphite through chemical oxidation and ultrasound. Due to the different oxidation conditions, the size of the obtained graphene oxide is generally between nanometers to hundreds of nanometers or even micrometers. Graphene oxide contains a large number of oxygen-containing groups. In recent years, the application prospects of graphene derivatives in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B31/04
Inventor 朱利民陶磊杨卉卉公晓曹欣乐
Owner DONGHUA UNIV
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