Method for manufacturing novel biological cartilage support by using umbilical cord Wharton jelly

Abstract

The invention discloses a method for manufacturing a novel biological cartilage support by using umbilical cord Wharton jelly. The method comprises the following steps of: treating an umbilical cord specimen to manufacture a novel biological cartilage support; performing paraffin embedding on a support material, slicing, performing H ematine (HE) dyeing, and observing a porous structure of the material under a light microscope; slicing the manufactured three-dimensional porous support into slices of 50 micrometers, spraying platinum on the surfaces of the slices, observing under a scanning electron microscope, shooting a scanned photograph by selecting appropriate multiples, and observing the appearance of the composite support material; and performing cell-free treatment on the umbilical cord Wharton jelly by combining a physical method and a chemical method, forming by using a freeze-drying method to manufacture the three-dimensional porous sponge-shaped support, wherein cells are removed completely, and a collagen structure is not damaged. According to the method, the umbilical cord Wharton jelly is subjected to the cell-free treatment by combining the physical method and the chemical method, and is formed by a freeze-drying method to manufacture the three-dimensional porous sponge-shaped support successfully, the cells are removed completely, and the collagen structure is not damaged, so that the three-dimensional porous sponge-shaped support is suitable to be used as a support carrier in cartilage tissue engineering.

Description

Technical field [0001] The invention belongs to the technical field of biological cartilage scaffolds, and in particular relates to a new biological cartilage scaffold made of umbilical cord Wharton's glue as a material. Background technique [0002] Articular cartilage damage caused by various reasons such as trauma, tumor, infection and degeneration is common in clinical practice. However, articular chondrocytes are terminally differentiated cells, and the proportion of chondrocytes in cartilage tissue is small (less than 1 %), cartilage itself lacks the distribution of blood vessels, nerves and lymphatic vessels, so the self-repair ability of cartilage tissue is low. [0003] Traditionally, commonly used methods to repair cartilage damage include joint cavity debridement, arthroplasty, subchondral bone drilling and microfracture. However, the results of using these techniques to repair cartilage damage have so far been unsatisfactory because The repair tissue formed is o...

AI Technical Summary

Problems solved by technology

[0002] Articular cartilage damage due to various reasons such as trauma, tumor, infection, and degeneration is common in clinical practice. However, articular chondrocytes are terminally differentiated cells, and the proportion of chondrocytes in cartilage tissue is small (less than 1 %), and the cartilage itself lacks the distribution of blood vessels, nerves and lymphatic vessels, so the self-repair ability of cartilage tissue is low

[0003] Traditionally, commonly used methods for repairing cartilage damage include joint cavity debridement, arthroplasty, subchondral bone drilling, and microfracture, but the use of these techniques to repair cartilage damage has not been satisfactory so far because The repair tissue formed by it is often not hyaline cartilage but fibrocartilage, which affects the repair effect and joint function

However, autologous bone cartilage, periosteum or perichondrium transplantation can cause additional damage, and its source is limited; although allogeneic tissue comes from a wide range of sources, there is a problem of rejection that is difficult to solve

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