Production method of three-dimensional porous composite bar

Abstract

The invention discloses a production method of a three-dimensional porous composite bar. The method is characterized by comprising the following steps of: preparing a graphene oxide solution; adding calcium salt and phosphate into the graphene oxide aqueous solution to be uniformly stirred, thus obtaining a graphene oxide / hydroxyapatite precursor solution; and adding konjac glucomannan into the graphene oxide / hydroxyapatite precursor solution under stirring, stirring the solution for 10-120 minutes at 35-60 DEG C, standing, filling the obtained mixed solution into a dialysis bag, soaking the sealed dialysis bag into alkaline solidification liquid, soaking the dialysis bag for 5-15 days at 40-60 DEG C, soaking materials in the dialysis bag in deionized water, cleaning and drying the materials, freezing the materials at a super low temperature and performing vacuum freeze drying on the materials to obtain the three-dimensional porous composite bar. The mechanical property and porosity of the produced composite bar can meet the clinical use requirements of bone repair scaffold materials, and the composite bar can effectively promote bone tissues to quickly and spontaneously repair defects and has a good effect.

Description

technical field

[0001] The invention belongs to the preparation of polymer composite rods, and relates to a preparation method of graphene oxide reinforced modified konjac glucomannan / hydroxyapatite three-dimensional porous composite rods. The mechanical properties and porosity of the prepared three-dimensional porous composite rod can meet the clinical application requirements of bone repair scaffold materials, and are especially suitable as bone repair scaffold materials. Background technique

[0002] Graphene oxide is an important derivative of graphene, also known as functionalized graphene. Its structure is roughly the same as that of graphene, except that there are some functional groups on the two-dimensional base surface, mainly some oxygen-containing functional groups. , such as hydroxyl, carboxyl, epoxy and carbonyl and many other active functional groups, where the hydroxyl and epoxy functional groups are mainly located on the basal plane of graphite, while the ca...

Examples

Embodiment 1

[0024] The graphene oxide powder was added to 250 mL of deionized water solution, and stirred ultrasonically for 1 hour to obtain a graphene oxide solution with a concentration of 1 mg / mL. Calcium nitrate and potassium dihydrogen phosphate are added to the graphene oxide solution according to the calcium ion concentration of 0.4kg / L and the phosphorus ion concentration of 0.18kg / L, and stir evenly to obtain the graphene oxide hydroxyapatite precursor solution. While stirring, 7.5 g of konjac glucomannan was added to obtain a mixed solution, stirred at a constant temperature of 45° C. for 30 minutes, and left to stand for defoaming for 24 hours. Inject the above solution into a dialysis bag, seal it with a clip of the dialysis bag, intrude into an alkaline coagulation solution with a mass concentration of 1.5%, soak at a constant temperature of 60°C for 7 days, and obtain graphene oxide modified konjac glucomannan / hydroxyapatite Stone three-dimensional porous composite gel, soa...

Embodiment 2

[0027] The graphene oxide powder was added to 250 mL of deionized water solution, and stirred ultrasonically for 0.5 hours to obtain a graphene oxide solution with a concentration of 1 mg / mL. Calcium nitrate and potassium dihydrogen phosphate are added to the graphene oxide solution according to the calcium ion concentration of 1.6kg / L and the phosphorus ion concentration of 0.72kg / L, and stir evenly to obtain the graphene oxide hydroxyapatite precursor solution. While stirring, 7.5 g of konjac glucomannan was added to obtain a mixed solution, stirred at a constant temperature of 45° C. for 30 minutes, and left to stand for defoaming for 24 hours. Inject the above solution into a dialysis bag, seal it with a clip of the dialysis bag, intrude into an alkaline coagulation solution with a mass concentration of 1.5%, soak at a constant temperature of 60°C for 7 days, and obtain graphene oxide modified konjac glucomannan / hydroxyapatite Stone three-dimensional porous composite gel, ...

Embodiment 3

[0030] The graphene oxide powder was added to 250 mL of deionized water solution, and stirred ultrasonically for 0.6 hours to obtain a graphene oxide solution with a concentration of 5 mg / mL. Calcium nitrate and potassium dihydrogen phosphate are added to the graphene oxide solution according to the calcium ion concentration of 1.6kg / L and the phosphorus ion concentration of 0.72kg / L, and stir evenly to obtain the graphene oxide hydroxyapatite precursor solution. Add 10 g of konjac glucomannan while stirring to obtain a mixed solution, stir at a constant temperature of 45° C. for 30 minutes, and let it stand for 24 hours for defoaming. Inject the above solution into a dialysis bag, seal it with a clip of the dialysis bag, intrude into an alkaline coagulation solution with a mass concentration of 1.5%, soak at a constant temperature of 60°C for 7 days, and obtain graphene oxide modified konjac glucomannan / hydroxyapatite Stone three-dimensional porous composite gel, soaked in 2 ...

technical field

[0001] The invention belongs to the preparation of polymer composite rods, and relates to a preparation method of graphene oxide reinforced modified konjac glucomannan / hydroxyapatite three-dimensional porous composite rods. The mechanical properties and porosity of the prepared three-dimensional porous composite rod can meet the clinical application requirements of bone repair scaffold materials, and are especially suitable as bone repair scaffold materials. Background technique

[0002] Graphene oxide is an important derivative of graphene, also known as functionalized graphene. Its structure is roughly the same as that of graphene, except that there are some functional groups on the two-dimensional base surface, mainly some oxygen-containing functional groups. , such as hydroxyl, carboxyl, epoxy and carbonyl and many other active functional groups, where the hydroxyl and epoxy functional groups are mainly located on the basal plane of graphite, while the ca...

Examples

Embodiment 1

[0024] The graphene oxide powder was added to 250 mL of deionized water solution, and stirred ultrasonically for 1 hour to obtain a graphene oxide solution with a concentration of 1 mg / mL. Calcium nitrate and potassium dihydrogen phosphate are added to the graphene oxide solution according to the calcium ion concentration of 0.4kg / L and the phosphorus ion concentration of 0.18kg / L, and stir evenly to obtain the graphene oxide hydroxyapatite precursor solution. While stirring, 7.5 g of konjac glucomannan was added to obtain a mixed solution, stirred at a constant temperature of 45° C. for 30 minutes, and left to stand for defoaming for 24 hours. Inject the above solution into a dialysis bag, seal it with a clip of the dialysis bag, intrude into an alkaline coagulation solution with a mass concentration of 1.5%, soak at a constant temperature of 60°C for 7 days, and obtain graphene oxide modified konjac glucomannan / hydroxyapatite Stone three-dimensional porous composite gel, soa...

Embodiment 2

[0027] The graphene oxide powder was added to 250 mL of deionized water solution, and stirred ultrasonically for 0.5 hours to obtain a graphene oxide solution with a concentration of 1 mg / mL. Calcium nitrate and potassium dihydrogen phosphate are added to the graphene oxide solution according to the calcium ion concentration of 1.6kg / L and the phosphorus ion concentration of 0.72kg / L, and stir evenly to obtain the graphene oxide hydroxyapatite precursor solution. While stirring, 7.5 g of konjac glucomannan was added to obtain a mixed solution, stirred at a constant temperature of 45° C. for 30 minutes, and left to stand for defoaming for 24 hours. Inject the above solution into a dialysis bag, seal it with a clip of the dialysis bag, intrude into an alkaline coagulation solution with a mass concentration of 1.5%, soak at a constant temperature of 60°C for 7 days, and obtain graphene oxide modified konjac glucomannan / hydroxyapatite Stone three-dimensional porous composite gel, ...

Embodiment 3

[0030] The graphene oxide powder was added to 250 mL of deionized water solution, and stirred ultrasonically for 0.6 hours to obtain a graphene oxide solution with a concentration of 5 mg / mL. Calcium nitrate and potassium dihydrogen phosphate are added to the graphene oxide solution according to the calcium ion concentration of 1.6kg / L and the phosphorus ion concentration of 0.72kg / L, and stir evenly to obtain the graphene oxide hydroxyapatite precursor solution. Add 10 g of konjac glucomannan while stirring to obtain a mixed solution, stir at a constant temperature of 45° C. for 30 minutes, and let it stand for 24 hours for defoaming. Inject the above solution into a dialysis bag, seal it with a clip of the dialysis bag, intrude into an alkaline coagulation solution with a mass concentration of 1.5%, soak at a constant temperature of 60°C for 7 days, and obtain graphene oxide modified konjac glucomannan / hydroxyapatite Stone three-dimensional porous composite gel, soaked in 2 ...

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