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Preparation technology of biphasic bone and cartilage tissue engineering scaffold material

A tissue engineering scaffold and preparation process technology, applied in medical science, prosthesis, etc., can solve the problem that the scaffold microstructure does not consider the complex layered structure of natural tissue, and achieve uniform pore size distribution, good biocompatibility, and repair effect. good effect

Inactive Publication Date: 2013-01-16
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there have been many reports on osteochondral tissue engineering scaffolds at home and abroad, but these scaffolds only distinguish the scaffolds of bone layer and cartilage layer in terms of material type or composition ratio, while the microstructure of the scaffolds does not take into account the complex hierarchical structure of natural tissues

Method used

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  • Preparation technology of biphasic bone and cartilage tissue engineering scaffold material
  • Preparation technology of biphasic bone and cartilage tissue engineering scaffold material
  • Preparation technology of biphasic bone and cartilage tissue engineering scaffold material

Examples

Experimental program
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Embodiment 1

[0025] A preparation process for dual-phase osteochondral tissue engineering scaffold material, comprising the following steps:

[0026] Step 1: Add chitosan and collagen powder to the acetic acid solution with a mass concentration of 2%, mix and stir, mix the collagen and chitosan in any proportion, configure the ratio of the collagen and chitosan mixture to the acetic acid aqueous solution to be 2.1g: 100ml of the solution, stored in a 4°C refrigerator for later use;

[0027] Step 2: Dissolve polylactic acid (PLA) in 1,4-dioxane solvent, prepare a 6%wt PLA solution, take bone powder and add it to the PLA solution, the amount of bone powder added is the same as the quality of PLA powder, mix and stir Uniform, bone powder adopts nanocrystalline calcium phosphate collagen-based bone repair material;

[0028] Step 3, take a dry through-hole polytetrafluoroethylene mold, block the hole from one end of the mold with a polytetrafluoroethylene cylinder with the same diameter as the...

Embodiment 2

[0033] A preparation process for dual-phase osteochondral tissue engineering scaffold material, comprising the following steps:

[0034] Step 1, add chitosan and collagen powder to the acetic acid solution with a mass concentration of 2%, mix and stir, the collagen and chitosan are mixed in any proportion, and the ratio of the collagen and chitosan mixture to the aqueous acetic acid solution is 3.5g: 100ml of the solution, stored in a 4°C refrigerator for later use;

[0035] Step 2: Dissolve polylactic acid (PLA) in 1,4-dioxane solvent, prepare 8%wt PLA solution, take bone powder and add it to PLA solution, the amount of bone powder added is the same as the quality of PLA powder, mix and stir Uniform, bone powder adopts nanocrystalline calcium phosphate collagen-based bone repair material;

[0036] Step 3, take a dry through-hole polytetrafluoroethylene mold, block the hole from one end of the mold with a polytetrafluoroethylene cylinder with the same diameter as the through ...

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Abstract

The invention discloses a preparation technology of biphasic bone and cartilage tissue engineering scaffold material. Firstly chitosan and collagen powder are added into acetate solution and laid in a refrigerator to be preserved; then PLA solution is prepared, and bone dust is put into the PLA solution and is uniformly mixed and stirred; then prepared PLA / bone dust solution is injected into a die to be prefrozen; then collagen / chitosan solution is added into the prefrozen die to be prefrozen, and a scaffold sample is obtained after freeze drying; and finally the bone and cartilage biphasic scaffold conducted to freeze drying is taken out from the die. The preparation technology ensures that a bone layer scaffold has the hardness and the strength approximate to that of natural bone tissue, is not only beneficial to the repair of cartilage tissue, but also convenient for clinically operating and fixing the scaffold; and meanwhile, a cartilage layer scaffold is provided with a multilayer structure containing perpendicular pore canals, is convenient for guiding cells on the scaffold to grow at a state close to that of a natural cartilage laminated structure, and improves the injury repair effect of cartilage.

Description

technical field [0001] The invention relates to the technical field of preparation of osteochondral tissue engineering scaffolds, in particular to a preparation process of dual-phase osteochondral tissue engineering scaffold materials. Background technique [0002] Articular cartilage is composed of single chondrocytes, dense collagen and glycoprotein matrix. Its main function is to disperse and transmit loads between joints, reduce friction, and enable joints to perform smooth and painless activities. Articular cartilage has no blood vessels, lymphatic vessels, and innervation. Chondrocytes are terminally differentiated cells, so the self-repair ability of cartilage is poor. Various injuries, inflammations, and degenerations can cause irreversible cartilage damage, which seriously affects joint function and quality of life. . [0003] The repair methods currently used in clinical practice all have obvious defects. The repaired cartilage tissue is different from natural car...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/36
Inventor 孙晓丹单程李程王梦哲林建华赖建明
Owner TSINGHUA UNIV
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