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Cartilage tissue engineering scaffold material and preparation method thereof

A scaffold material and cartilage tissue technology, applied in the field of cartilage tissue engineering scaffolds and their preparation

Inactive Publication Date: 2014-03-19
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] One of the objectives of the present invention is to overcome the problem that the calcium ions that play a cross-linking role in the sodium alginate / chitosan electrostatic composite scaffold existing in the prior art are replaced by sodium ions, so as to provide a cartilage tissue engineering scaffold material

Method used

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  • Cartilage tissue engineering scaffold material and preparation method thereof

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Embodiment 1

[0025] Prepare a 2.0% sodium alginate solution with deionized water, prepare a 2.0% chitosan solution with acetic acid solution, add 0.0641 g of 1-ethyl-(3-dimethyl Aminopropyl) carbodiimide hydrochloride (EDC??HCl) and 0.0077g of N-hydroxysuccinimide (NHS), stirring for 10 to 40 minutes; add the activated sodium alginate solution to 1mL shell In the polysaccharide solution, continue to stir slowly; after the viscosity changes slightly, pour it into a mold, freeze at -20°C for 12 to 24 hours, and then freeze-dry to obtain a sodium alginate / chitosan three-dimensional porous scaffold. The performance of the bracket is as follows after testing: the porosity is 94%; the pore diameter is 160-220?m.

Embodiment 2

[0027] Prepare the mass fraction of 2.0% sodium alginate solution with deionized water, prepare the mass fraction of 2.0% chitosan solution with acetic acid solution, add 0.1069g of 1-ethyl-(3-dimethyl Aminopropyl) carbodiimide hydrochloride (EDC??HCl) and 0.0128g of N-hydroxysuccinimide (NHS), stirring for 10 to 40 minutes; add the activated sodium alginate solution to 1mL shell In the polysaccharide solution, continue to stir slowly; after the viscosity changes slightly, pour it into a mold, freeze at -20°C for 12 to 24 hours, and then freeze-dry to obtain a sodium alginate / chitosan three-dimensional porous scaffold. The performance of the stent is as follows after testing: the porosity is 94%; the pore diameter is 170-220??m.

Embodiment 3

[0029] Prepare a 2.0% sodium alginate solution with deionized water, prepare a 2.0% chitosan solution with acetic acid solution, add 0.0641 g of 1-ethyl-(3-dimethyl Aminopropyl) carbodiimide hydrochloride (EDC??HCl) and 0.0077g of N-hydroxysuccinimide (NHS), stirring for 10 to 40 minutes; add the activated sodium alginate solution to 1mL shell In the polysaccharide solution, continue to stir slowly; after the viscosity changes slightly, pour it into a mold, freeze at -80°C for 12 to 24 hours, and then freeze-dry to obtain a sodium alginate / chitosan three-dimensional porous scaffold. The performance of the stent is as follows after testing: the porosity is 93%; the pore diameter is 80-120?m.

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Abstract

The invention discloses a cartilage tissue engineering scaffold material and a preparation method thereof. The scaffold material is a three-dimensional porous structure formed by using sodium alginate and chitosan as a base material, and enabling the carboxyl on a side chain of the sodium alginate activated by activation cross-linking agent to react with amino group of chitosan, wherein molar ratio of the sodium alginate carboxyl to the chitosan amino group is (3:7)-(7:3), and the final product only contains two components, sodium alginate and chitosan. The three-dimensional porous scaffold provided by the invention has good biocompatibility, and is suitable for the cartilage tissue engineering.

Description

technical field [0001] The invention relates to a cartilage tissue engineering scaffold and a preparation method thereof. technical background [0002] Cartilage is one of the important tissues of the human body. Cartilage defects due to trauma, wear, and abnormal development often appear clinically. Small areas of cartilage defects can usually heal by themselves, but because there are no blood vessels in the cartilage tissue, large-scale cartilage defects usually cannot heal by themselves, and there is a lack of effective treatment methods in clinical practice. Autologous cartilage transplantation is limited by the limitation of its own donors; allogeneic cartilage transplantation has severe antigenic immune rejection and the risk of cross-infection. Tissue engineering provides an effective and feasible way for cartilage defect repair. [0003] Currently, cartilage tissue engineering scaffold materials mainly include synthetic polymers and natural polymers. Synthetic po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/58A61L27/40
Inventor 尹静波张伟骏潘建兵徐欢伍芳芳张坤玺
Owner SHANGHAI UNIV
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