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Preparation method of silk fibroin and chitosan three-dimensional scaffold material

A technology of three-dimensional scaffold and white chitosan, which is applied in the fields of pharmaceutical formulation, medical science, tissue regeneration, etc., can solve the problems of fragility and slow degradation

Inactive Publication Date: 2016-04-27
SHAANXI YUHANG ELECTRONICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of studies have shown that silk fibroin and chitosan are natural polymer materials, non-toxic and tasteless, and have good biological and physical and chemical properties. However, when used alone, they all expose some shortcomings, such as brittle when dry, slow degradation, etc.

Method used

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specific Embodiment

[0010] Specific embodiments: the preparation process of the present invention includes two steps of silk fibroin extraction and three-dimensional scaffold material preparation;

[0011] The step of extracting the silk fibroin is to add 0.5%Na 2 CO 3 and 3g / L detergent solution, after degumming, rinsing and drying, silk fibroin is obtained, and silk fibroin is dissolved in 30% ethanol and 40% CaCl by volume fraction at 1:20 (g:mL) 2 Dissolve completely in a water bath at 80°C, filter to obtain the silk fibroin stock solution, and dialyze with a cellulose semipermeable membrane dialysis bag for 3 days to remove calcium chloride and ethanol, and stir the silk fibroin stock solution at 50°C Concentrate, filter, centrifuge, remove the insoluble part, obtain silk fibroin solution, and measure its concentration;

[0012] The preparation step of the three-dimensional scaffold material is as follows: fully dissolving chitosan in 2% acetic acid, mixing silk fibroin and chitosan at a m...

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Abstract

The invention discloses a preparation method of a silk fibroin / chitosan three-dimensional scaffold material, and belongs to the field of the preparation of a cartilage tissue engineering scaffold material. The invention provides a method for preparing the three-dimensional scaffold material from silk fibroin and chitosan. The method comprises the following steps: firstly, preparing the silk fibroin; and then, mixing and processing the silk fibroin and the chitosan, so that the silk fibroin / chitosan three-dimensional scaffold material is obtained. The silk fibroin / chitosan three-dimensional scaffold material prepared by the method conforms to the requirements on the physicochemical properties of the cartilage tissue engineering scaffold material; and the scaffold material is expected to become a relatively ideal scaffold material for cartilage tissue engineering.

Description

technical field [0001] The invention belongs to the field of cartilage tissue engineering support material preparation. Background technique [0002] Since Langer and Vacanti proposed the concept of tissue engineering in the 1980s, it has brought new hope to the treatment of many patients with tissue defects, and it has also become a hot research topic for scholars. Since the emergence of cartilage tissue engineering, it has brought new light to the repair of cartilage. Scaffold materials are one of the difficulties that urgently need to be solved in the research of cartilage tissue engineering. A large number of studies have shown that silk fibroin and chitosan are natural polymer materials, non-toxic and tasteless, and have good biological and physical and chemical properties. However, when used alone, they all expose some shortcomings, such as brittle when dry, slow degradation, etc. . Contents of the invention [0003] The purpose of the present invention is to prov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/56A61L27/50
CPCA61L27/26A61L27/50A61L27/56A61L2400/08A61L2430/06
Inventor 郝青
Owner SHAANXI YUHANG ELECTRONICS
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