Cartilage tissue engineering bracket and preparation method thereof

A cartilage tissue and engineering technology, applied in the field of cartilage tissue engineering scaffolds and their preparation, can solve problems such as poor mechanical strength, and achieve the effects of high practicability, high mechanical strength and inducing ability, and promoting adhesion

Active Publication Date: 2014-06-25
PEKING UNIV THIRD HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the problem of poor mechanical strength of the cartilage tissue engineering scaffold in the p...

Method used

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  • Cartilage tissue engineering bracket and preparation method thereof
  • Cartilage tissue engineering bracket and preparation method thereof
  • Cartilage tissue engineering bracket and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0042] The embodiment of the present invention provides a cartilage tissue engineering scaffold 3 , the components of which include: natural polymer hydrogel 1 and demineralized bone matrix 2 .

[0043] The embodiment of the present invention provides a cartilage tissue engineering scaffold 3 prepared from natural polymer hydrogel 1 and decalcified bone matrix 2, by combining natural polymer hydrogel 1 with decalcified bone matrix 2, on the one hand , the cartilage tissue engineering scaffold 3 has the plasticity, adhesion and injectability of the hydrogel, so that it has various advantages brought by the natural biological scaffold material itself; on the other hand, the demineralized bone matrix 2 can The collagen fiber network framework is provided, which endows the prepared cartilage tissue engineering scaffold 3 with higher mechanical strength and induction ability, and further promotes the adhesion, proliferation and differentiation of cells.

Embodiment 2

[0045] The embodiment of the present invention provides a cartilage tissue engineering scaffold 3 , the components of which include: natural polymer hydrogel 1 and demineralized bone matrix 2 .

[0046] Natural polymer hydrogel 1 has high water content, plasticity and injectability, and is a commonly used material in tissue engineering research. The biggest advantage of natural polymer hydrogel 1 is that it can maintain the spherical chondrocyte phenotype of the encapsulated cells and prevent dedifferentiation. It can be designed into a liquid form to make it injectable, and it can be better mixed with cartilage repair cells to avoid cell loss. It can be seen that the injectability and high adhesion of the natural polymer hydrogel 1 enable it to be widely used in the field of minimally invasive medicine. In addition, because the hydrogel can swell in water and retain a large amount of water without dissolving, it is very similar to the body tissue filled with a large amount o...

Embodiment 3

[0052] as attached figure 1 Shown, the embodiment of the present invention provides a kind of preparation method of cartilage tissue engineering support 3, and this method comprises:

[0053] Step 101: Prepare demineralized bone matrix 2.

[0054] Step 102: Prepare natural polymer hydrogel solution.

[0055] Step 103 injecting the natural polymer hydrogel solution into the decalcified bone matrix 2, allowing the natural polymer hydrogel solution to completely penetrate into the demineralized bone matrix 2, and performing a gelation reaction at a preset temperature to obtain Cartilage Tissue Engineering Scaffold3.

[0056] Wherein, the above-mentioned making the natural polymer hydrogel solution completely penetrate into the decalcified bone matrix 2 refers to an ideal state in which the natural polymer hydrogel solution completely fills any gap in the decalcified bone matrix 2 .

[0057] The embodiment of the present invention also provides a preparation method of a cartila...

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Abstract

The invention discloses a cartilage tissue engineering bracket and a preparation method thereof, and belongs to the field of cartilage repair. Components of the bracket comprise natural high polymer water-gel and decalcified bone matrix. By combining the natural high polymer water-gel with the decalcified bone matrix, the bracket not only has plasticity, adhesion and injection of the water-gel, but also has various advantages brought by the natural biological bracket material; and moreover, the decalcified bone matrix can provide a collagenous fiber net rack, and provides higher mechanical strength and inducibility for the prepared bracket, and further promotes adhesion, multiplication and differentiation of the cells. The invention further discloses the preparation method of the cartilage tissue engineering bracket, which comprises steps of injecting prepared natural high polymer water-gel liquor in the decalcified bone matrix, so that the natural high polymer water-gel is completely infiltrated into gaps of the decalcified bone matrix, and carrying out gelatinization reaction at a preset temperature to obtain the cartilage tissue engineering bracket with higher mechanical strength and higher inducibility. The method is simple, easy to operate and high in practicability.

Description

technical field [0001] The invention relates to the field of cartilage repair, in particular to a cartilage tissue engineering scaffold and a preparation method thereof. Background technique [0002] Cartilage tissue engineering is a technology that uses tissue engineering methods to construct cartilage composite tissues. The three basic elements in cartilage tissue engineering are: seed cells, degradable cartilage tissue engineering scaffolds, and cell growth regulators. Among them, the cartilage tissue engineering scaffolds play an important role in the process of cartilage repair. Cartilage tissue engineering scaffolds are mainly used to provide a three-dimensional space structure for the construction of chondrocytes, which is conducive to cell adhesion and proliferation, and provides a good growth environment for cell growth. [0003] At present, commonly used cartilage tissue engineering scaffolds are mainly prepared from natural biological scaffold materials, such as ...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/22A61L27/24A61L27/52A61L27/36A61L27/56
Inventor 敖英芳黄洪杰张辛胡晓青满振涛周春燕
Owner PEKING UNIV THIRD HOSPITAL
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