The invention discloses a preparation method of a chitosan hydrogel stent for tissue engineering. The preparation method comprises the steps of: preparing a crosslinking agent and preparing gel and specifically comprises the following steps of: 1, preparing the crosslinking agent, placing 1,6-diisocyanatohexane and polyethylene glycol in a container according to a mol ratio of (1-2.5):1, reacting for 1-7 days at a temperature of 20-40 DEG C to obtain the crosslinking agent; 2, preparing a chitosan hydrogel stent for tissue engineering, (A) preparing a chitosan water solution with a concentration of 0.1-10 percent in weight ratio; and (B), adding the crosslinking agent in the chitosan water solution to obtain the chitosan hydrogel stent for tissue engineering, wherein the volume proportion of the crosslinking agent to the chitosan water solution is 1000:(1-50). Compared with the prior art, the preparation method has the advantages of simple and controllable process, mild reaction conditions, no need of catalyst, high speed and better property of chitosan hydrogel, and good biocompatibility because infinitesimal chemical crosslinking agent is introduced during crosslinking, and is a better tissue engineering stent material, and is especially suitable for a soft tissue engineering material.