Three-dimensional chitosan hydrogel and preparation method thereof

A three-dimensional chitosan water, chitosan technology, applied in pharmaceutical formulations, medical preparations with inactive ingredients, prostheses, etc., can solve the problems of increasing the preparation process time and cost, long operation time, etc., and achieve a good biological phase. Capacitive and biodegradable, short reaction time, easy molding effect

Active Publication Date: 2012-07-25
SHANGHAI HUAYI BIO LAB CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, these existing methods have defects of one kind or another, or the operation time is too long, such as the freeze-drying method; The residual amount of toxic substances in the final product needs to be strictly tested and controlled, such as solvent replacement method and gas foaming method, which will also increase the time and cost of the preparation process

Method used

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  • Three-dimensional chitosan hydrogel and preparation method thereof
  • Three-dimensional chitosan hydrogel and preparation method thereof
  • Three-dimensional chitosan hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Preparation of Chitosan Porous Hydrogel I

[0050] Put 1.5 g of chitosan into 90 ml of deionized water, and magnetically stir at room temperature to make the chitosan evenly dispersed in the water. Slowly add 10 ml of acetic acid-phosphate buffer solution dropwise to the dispersion, adjust the pH to 6.5, and completely dissolve the chitosan, pour it into a mold, put it into a high-temperature incubator, and carry out high-temperature cross-linking at 80 °C for 30 minutes to obtain Porous chitosan hydrogel crude product.

[0051] The chitosan hydrogel was soaked and rinsed with deionized water to remove the acid residue, and a three-dimensional porous chitosan hydrogel scaffold material I was obtained. see attached figure 1 a.

[0052] The prepared three-dimensional chitosan hydrogel I is disc-shaped with an average pore size of 30 μm, see attached figure 1 b.

Embodiment 2

[0054] Preparation of Chitosan Porous Hydrogel II

[0055] Put 0.2 g of chitosan into 9 ml of deionized water, and magnetically stir at room temperature to make the chitosan evenly dispersed in the water. Slowly add 1 ml of hydrochloric acid-phosphate buffer solution dropwise to the dispersion, adjust the pH to 7, and completely dissolve the chitosan, pour it into a mold, put it into a high-temperature incubator, and carry out high-temperature cross-linking at 121 ° C for 30 minutes to obtain Porous chitosan hydrogel crude product.

[0056] The chitosan hydrogel was soaked and rinsed with deionized water to remove the acid residue, and a three-dimensional porous chitosan hydrogel scaffold material I was obtained.

[0057] The obtained three-dimensional chitosan hydrogel II is disc-shaped with an average pore diameter of 100 μm, see attached figure 2 .

Embodiment 3

[0059] Preparation of Chitosan Porous Hydrogel III

[0060] Put 0.2 g of chitosan into 9 ml of deionized water, and magnetically stir at room temperature to make the chitosan evenly dispersed in the water. Slowly add 1 ml of acetate-phosphate buffer solution dropwise to the dispersion, adjust the pH to 6.3, and pour the chitosan into the mold after completely dissolving it. A crude product of porous chitosan hydrogel was obtained.

[0061] The chitosan hydrogel was soaked and rinsed with deionized water to remove the acid residue to obtain a three-dimensional porous chitosan hydrogel scaffold III with an average pore size of 20 μm. image 3 .

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Abstract

The invention discloses a method for preparing three-dimensional chitosan hydrogel by using a high temperature crosslinking method and the obtained chitosan hydrogel. The method comprises the following steps of: (a) dispersing chitosan powder into a deionized water; (b) adjusting the pH of chitosan dispersion obtained in the step (a) to be subacid; (c) carrying out high temperature crosslinking on the solution obtained in the step (b) at the high temperature of 80-125 DEG C; and (d) after the crosslinking reaction is completed, soaking and cleaning an obtained product by using a posttreatment liquid so as to remove a residual acid liquid. The high temperature crosslinking method is simple in the preparation process; the reaction time is short; raw materials are unnecessary for special treatment; highly-toxic chemical substances such as crosslinking agents are not introduced to a preparation course; no any chemical toxicant remains in a final product; and the three-dimensional chitosan hydrogel is even in pore structure, good in connectivity and easy to form, and is very suitably used as a support material of soft tissue engineering such as skin and cartilage.

Description

technical field [0001] The invention relates to a three-dimensional scaffold material in the technical fields of biomedical engineering and tissue engineering and a preparation method thereof. The three-dimensional scaffold material can be used for three-dimensional cell culture and in vitro amplification, and controlled release of biologically active substances. Background technique [0002] The three-dimensional scaffold material plays a key role in tissue engineering. It can be used as a carrier for cell culture and proliferation in vitro. After the cells are implanted with the scaffold material in the body, they continue to proliferate in the in vivo environment and interact with host cells and tissues to form new cells. The tissue, and the material is degraded and excreted with metabolism to achieve the effect of tissue repair. [0003] Three-dimensional scaffold materials for tissue engineering include collagen, chitosan, and polyvinyl alcohol. Among them, chitosan is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/36C08J3/075C08J3/24C08L5/08A61L27/52A61L27/60A61L27/20A61K47/36
Inventor 吉承栋
Owner SHANGHAI HUAYI BIO LAB CO LTD
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