Process for preparing porous support frame of soft tissue engineering with water-soluble high-molecular material as mould

A technology of water-soluble polymers and porous scaffolds, applied in the field of biomedical engineering, can solve problems such as inability to manufacture shapes, difficult demoulding, complex scaffolds, etc., and achieve realistic shapes, short manufacturing cycles, and adjustable pore diameters

Inactive Publication Date: 2002-06-26
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the known technology, only regular-shaped polyhydroxyalkanoate (PHA) three-dimensional porous scaffolds can be manufactured, such as thin slices, cylinders, cuboids and other shapes (such as the published patent "a porous scaffold for tissue and organ repair") "Preparation method" (Patent Publication No. CN1269247A) shown in the accompanying drawings), but can not manufacture many complex-shaped brackets, especially when the hard mold is used to make a bracket with an inner concave surface, it is difficult to demould

Method used

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  • Process for preparing porous support frame of soft tissue engineering with water-soluble high-molecular material as mould
  • Process for preparing porous support frame of soft tissue engineering with water-soluble high-molecular material as mould
  • Process for preparing porous support frame of soft tissue engineering with water-soluble high-molecular material as mould

Examples

Experimental program
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Effect test

Embodiment 1

[0032] 1. Turn over polyethylene glycol (PEG) negative mold: put PEG (average molecular weight 5000) into a beaker and heat to 70°C, melt it and pour it into a petri dish with a silicone positive mold facing upwards, so that the liquid The surface is flush with the edge of the male mold. After standing and solidifying, the soft male mold is removed to obtain the base of the PEG female mold. Fold along the positive contour of the male mold with smooth coated paper to make an open barrel-shaped container with the soft male mold as the bottom and the coated paper as its periphery. The molten PEG is poured into this opening, and after standing and solidifying, the male mold is peeled off to obtain a PEG female mold upper cover that matches the shape of the front of the male mold.

[0033] 2. Prepare poly-2-hydroxybutyrate (PHB) chloroform solution-NaCl particle mixture: use a series of standard sieves with a filtration range of 75 to 300 microns to screen out NaCl particles with ...

Embodiment 2

[0039] 1, remake polyethylene glycol (PEG) negative mold: use PEG (average molecular weight is 10000) mold making, following steps are with embodiment one.

[0040] 2. Preparation of 2-hydroxybutyric acid and 2-hydroxyvaleric acid block copolymer (PHBV) chloroform solution-NaCl particle mixture: use a series of standard sieves with a filtration range of 75 to 300 microns to screen out particle sizes between 100 and 150 Micron NaCl particles; weigh 1.0g of PHBV powder, put it in a round bottom flask, measure 10.0ml of chloroform and pour it into it, stir it with a polytetrafluoroethylene magnet in a water bath at 60°C, heat and reflux for 30 minutes to polymerize The substance is fully and evenly dissolved. Weigh 30 grams of NaCl particles and place them in a jar, pour the polymer solution into it, wrap the stopper of the jar tightly with polytetrafluoroethylene raw tape, make it airtight, and let it stand for 12 hours to make the solution fully infiltrate the NaCl particles. ...

Embodiment 3

[0043] 1, remake polyethylene glycol (PEG) negative mold: use PEG (average molecular weight is 20000) mold making, following steps are with embodiment one.

[0044] 2. Preparation of 2-hydroxybutyric acid and 2-hydroxyhexanoic acid block copolymer (PHBH) chloroform solution-sucrose particle mixture: use a series of standard sieves with a filtration range of 75 to 300 microns to screen out a particle size of 200 to 250 Micron sucrose particles; Weigh 1.0g of PHBH powder, put it in a round bottom flask, pour 10.0ml of chloroform into it, stir it with a Teflon magnet in a water bath at 60°C, heat and reflux for 30 minutes to polymerize The substance is fully and evenly dissolved. Weigh 30 grams of sucrose particles and place them in a wide-mouth bottle, pour the polymer solution into it, wrap the stopper of the wide-mouth bottle tightly with polytetrafluoroethylene raw tape, make it airtight, and let it stand for 12 hours to make the solution fully infiltrate the sucrose particle...

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Abstract

A porous support for cartrilage tissue engineering is prepared from the high-molecular material of polyethanediol, polyamide, or polyacrylic acid type as mould material, polyhydroxy alkanoate as polymer for the porous support chloroform as solvent and water-soluble crystal as pore-forming agent through smelting the said mould material, pouring it into a container in which there is a soft silica gel male mould, obtaining a high-molecular femal mould, filling the mixture of polymer solution and pore-forming agent particles in the said femal mould, closing compacting, baking to remove solvent, dissolving the mould and pore-forming particles, and drying.

Description

technical field [0001] The invention relates to a method for preparing a porous support for cartilage tissue engineering by using a water-soluble polymer as a mold, belonging to the field of biomedical engineering. Background technique [0002] There are a large number of cases of organ damage every year, and the usual solution is organ transplantation. The main problem with organ transplantation is the shortage of tissue and organ donors. Due to the shortage of organs, the majority of patients will not be able to receive effective treatment. Organ transplants have other disadvantages. Tissue engineering applies the principles and technologies of life science and engineering to design and manufacture functional artificial tissue substitutes to repair or reconstruct the functions of tissues and organs. The basic principle and method of tissue engineering is to adsorb normal tissue cells cultured and expanded in vitro to a biomaterial with good biocompatibility and absorbed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/14A61L27/56A61L31/04B29C33/52
Inventor 胡平陈江睿
Owner TSINGHUA UNIV
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