Tissue engineering cartilage based on placenta source filling dry cell and production thereof

A mesenchymal stem cell and tissue engineering technology, applied in embryonic cells, biochemical equipment and methods, tissue culture, etc., can solve the problems of lack of large-scale separation, purification and expansion methods of placenta-derived mesenchymal stem cells, and achieve convenient clinical Effect of application, high ultimate strength and stability, easy access to materials

Inactive Publication Date: 2006-03-15
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are only sporadic literatures at home and abroad about the small amount of placenta-derived adherent cells, which are mainly used for hematopoietic support research, but there is a lack of detailed technical information on the large-scale separation, purification and expansion of placenta-derived mesenchymal stem cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 The acquisition of human placenta-derived mesenchymal stem cells

[0020] Immediately after delivery, the placenta is placed in a sterile tray or container, injected with balanced salt solution containing anticoagulants and antibiotics via the umbilical artery, exsanguinated, and perfused. The anticoagulant can be 1~100u / mL heparin, and the antibiotic can be penicillin and streptomycin. Collect the perfusate and centrifuge to obtain cell components; or aseptically cut the placental leaflets after the initial perfusion, digest the placental tissue with trypsin or collagenase to obtain a cell suspension, and then pass the cell suspension through a cell sieve to remove tissue debris and blood cells, The obtained cells were resuspended, centrifuged at 500g for 30 minutes with percoll with a density of 1.073g / mL, harvested the interfacial cell layer, washed with PBS and inoculated into culture bottles or culture plates, or directly used for other purposes. It is a...

Embodiment 2

[0021] Example 2 A method for manufacturing tissue-engineered cartilage based on placenta-derived mesenchymal stem cells

[0022] Freshly isolated or cryopreserved human placenta-derived mesenchymal stem cells were prepared into 1.5-2×10 7 / ml of cell suspension, seeded on the cylindrical β-TCP scaffold, try to make the cells evenly distributed. The β-TCP porous bioceramic material can be produced by the patented method and process of Shanghai Biolu Company, and the material is passed through 60gray 60 Co irradiated and sterilized for construction of tissue-engineered articular cartilage. In a preferred solution, its microstructure is: pore diameter-100-300 μm, porosity-52%, inter-pore connection diameter-30-100 μm. After standing still for 6 hours, the complexes were transferred into RCCS (rotary bioreactor, Synthecom Company), and cultured in DMEM containing 10% FCS for 48 hours at a rotation speed of 30 rpm. The cultured complex can be directly used for clinical repair of...

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Abstract

A tissue engineering cartilage based on placenta original filled stem cell and its production are disclosed. The process is carried out by using filled stem cell of placenta original as seed cell, combining with degradable porous biological material to construct tissue engineering cartilage. It is convenient and has short cell culture time and easy quality control, it can be used for functional repair of joint cartilage injure.

Description

field of invention [0001] The invention relates to a tissue-engineered cartilage based on placenta-derived mesenchymal stem cells and a manufacturing method thereof, belonging to the field of tissue engineering. Background technique [0002] Articular cartilage trauma repair is still a great challenge in clinical medicine. Current treatment options include cartilage or bone-cartilage grafts and artificial joints. Autologous cartilage is the best transplant material, but it is not ideal in terms of application because of the limited body size and the need for surgery at a second site to obtain sufficient cartilage tissue for transplantation. However, the replacement repair of artificial joints has so far been difficult to achieve functional repair effects, and is often accompanied by various complications. Despite many years of unremitting efforts in this field, there is still a need to find an effective treatment for the repair of articular cartilage damage, providing appr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08C12N11/00A61F2/28C12N5/0735C12N5/0775
Inventor 郭希民王常勇周晓东段翠密江红董灵芝李晶
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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