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Preparation method of DA-based gradient functional material

A gradient functional material, dopamine-based technology, used in medical science, tissue regeneration, prosthesis, etc., to achieve the effect of promoting bone tissue regeneration performance, excellent cell adhesion, and excellent biocompatibility

Active Publication Date: 2017-01-04
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some problems that cannot be ignored in gradient materials, such as the simplification of the preparation method, the stability of the gradient structure, and the mechanical strength enough to support the tissue, etc.

Method used

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  • Preparation method of DA-based gradient functional material
  • Preparation method of DA-based gradient functional material
  • Preparation method of DA-based gradient functional material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1, the synthesis of alginic acid-dopamine (Alg-DA) modified macromolecule

[0016] Weigh 1 g of alginic acid (Alg), put it into a three-necked flask, add 100 mL of PBS buffer solution (50 mM, pH=5.5.), stir and dissolve at room temperature, and after it is completely dissolved, add 1.94 g of EDC and 2.32 g of NHS in sequence, n( COOH) / n(EDC) / n(NHS)=1 / 2 / 4, after activation at room temperature for 30 minutes, add 4.79 dopamine hydrochloride (DA), repeat vacuuming and nitrogen three times to exclude air to prevent DA oxidation, under nitrogen atmosphere React at room temperature for 24 hours. After stopping the reaction, dialyze in deionized water to remove the catalyst and unreacted monomers. After there is no dopamine ultraviolet absorption peak in the dialysate, freeze-dry to obtain a white cotton-like biomodified macromolecule Alg-DA.

Embodiment 2

[0017] Embodiment 2, the preparation of polydopamine particle (PDA)

[0018] Add 1.5mL of ammonia water to the mixed solution of ethanol (40mL) and ultrapure water (90mL), stir at 30°C for 30min, then dissolve 0.5g of dopamine in 10mL of ultrapure water and add to the above solution, at 30°C The reaction was carried out for 24 hours, and the obtained product was dialyzed and freeze-dried to obtain PDA particles.

Embodiment 3

[0019] Embodiment 3, preparation of dopamine-based gradient functional material

[0020] Alg-DA was dissolved in phosphate buffered saline (PBS) with a pH of 5.5 to prepare Alg-DA solutions with concentrations of 2%, 3% and 4%, respectively, and 20 mg of PDA particles were added to the above solutions, and mechanically stirred for 4 hours. First, 2mL of Alg-DA / PDA particles with a concentration of 4% were evenly added to the mold and quickly frozen with liquid nitrogen, and then 2mL of Alg-DA / PDA particles with a concentration of 3% were evenly poured on the first layer of frozen materials And freeze quickly, and finally add 2mL of Alg-DA / PDA particles with a concentration of 2% to the second layer of frozen material. After the third layer of solution is frozen, take out the material and put it in a freeze dryer to freeze dry , the lyophilized material was treated with 5% CaCl 2 Cross-linking is carried out, and the cross-linked sample is washed with ultrapure water and freez...

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Abstract

The invention provides a preparation method of a DA-based gradient functional material. DA serves as a functional group, and the chemical reactivity, oxidation auto-polymerization and adhesion of DA are utilized to prepare the functional material with a multistage pore channel and a gradient structure. The method includes the steps that firstly, Alg is subjected to graft modification with DA to prepare biologically-modified macromolecular Alg-DA with excellent adhesion performance; then, DA is subjected to oxidation auto-polymerization assembly in a slightly alkaline buffer solution to form PDA particles of a uniform particle size; then, Alg-DA of different concentrations and the PDA particles act on each other to form a first-stage cross-linked structure, and then the gradient functional material is prepared with a lamination freeze-drying method; then, the gradient functional material is cross-linked again with calcium ions, and the cross-linking degree and porosity of the material are further adjusted. The DA-based gradient functional material provides a new way for preparing composites with an ideal texture. The gradient functional material prepared through the method has high mechanical performance and excellent biocompatibility and biodegradability, can effectively improve the absorption separation performance and the regeneration performance of soft / hard tissue and has broad application prospects in the fields of multifunctional separation and absorption films and biomedicine.

Description

technical field [0001] The invention relates to a preparation method of a dopamine-based gradient functional material, which belongs to the technical field of polymer materials. [0002] technical background [0003] The structure of many materials in nature, such as wood, bamboo, bone, etc., is a typical porous gradient structure. It is a new type of functional material whose structure changes continuously from one side to the other side along the thickness direction, so that the properties and functions of the material also change continuously. The dense part of the pore structure in the gradient material makes the material have sufficient strength, and the loose part of the pore structure can effectively reduce the density of the material. Since different parts of gradient porous functional polymer materials have different structures and properties, they can be applied in the fields of building materials and multifunctional separation or absorption membranes. The biomimet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/58A61L27/50
CPCA61L27/20A61L27/50A61L27/58A61L2430/02C08L5/04
Inventor 施冬健沈佳丽章朱迎曹传昊陈晨李小杰陈明清
Owner JIANGNAN UNIV
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