Tissue culture rapid propagation method for mini core-fragrant sweet potatoes

A technology of mini sweet potato and tissue culture, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc. It can solve the problems of slow development, small planting scale, and unsatisfactory processing enterprises.

Inactive Publication Date: 2016-11-09
TIANJIN FENGHUA YULONG AGRI DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, in the process of producing mini Xinxiang sweet potatoes, affected by viral diseases, the development is slow, and the planting scale is small, which cannot meet the needs of processing enterprises.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for tissue culture and rapid propagation of mini sweet potato, the specific steps are:

[0018] (1) Materials and processing:

[0019] Harvest the young stems with buds from the field, remove the leaves and excess petioles, trim them into 0.3cm long stems with buds, cut off the top buds, first wash off the dust on the surface with a brush under running water and soil, soaked in 0.2% ascorbic acid solution for 6 min, washed with washing powder solution, rinsed with running water for 40 min, and disinfected with alcohol for 8 s under aseptic conditions to obtain explants, which are for later use;

[0020] (2) Explant disinfection and primary culture:

[0021] Sterilize the mini sweet potato explants with 0.2% mercuric chloride for 9 min; then put the treated explants into the primary medium for culture; the culture temperature is 25°C, and the culture time is 30 days. The light intensity of the first 15 days was 1600lx, 12 hours a day; the light intensity of th...

Embodiment 2

[0028] A method for tissue culture and rapid propagation of mini sweet potato, the specific steps are:

[0029] (1) Materials and processing:

[0030] Harvest the tender stems with buds from the field, remove the leaves and excess petioles, trim them into 0.4cm long stems with buds, cut off the top buds, and wash off the dust on the surface with a brush under running water. and soil, soaked in ascorbic acid solution of 0.2% concentration for 8min, washed with washing powder solution, rinsed with running water for 50min, and disinfected with alcohol for 9s under aseptic conditions to obtain explants for use;

[0031] (2) Explant disinfection and primary culture:

[0032] Sterilize the mini sweet potato explants with 0.2% mercuric chloride for 10 min; then put the treated explants into the primary medium for cultivation; the cultivation temperature is 28° C., and the cultivation time is 30 days. For the first 15 days, the light intensity was 1800lx, 12 hours a day; for the nex...

Embodiment 3

[0039] A method for tissue culture and rapid propagation of mini sweet potato, the specific steps are:

[0040] (1) Materials and processing:

[0041] Harvest the young stems with buds from the field, remove the leaves and excess petioles, trim them into 0.3cm long stems with buds, cut off the top buds, first wash off the dust on the surface with a brush under running water and soil, soaked in ascorbic acid solution of 0.2% concentration for 7min, washed with washing powder solution, rinsed with running water for 45min, and disinfected with alcohol for 9s under aseptic conditions to obtain explants, which are for subsequent use;

[0042] (2) Explant disinfection and primary culture:

[0043] Sterilize the mini sweet potato explants with 0.2% mercuric chloride for 10 min; then put the treated explants into the primary medium for culture; the culture temperature is 27°C, and the culture time is 30 days. The light intensity of the first 15 days was 1700lx, 12 hours a day; the l...

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PUM

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Abstract

The invention relates to a tissue culture rapid propagation method for mini core-fragrant sweet potatoes. The tissue culture rapid propagation method specifically includes: picking the young and tender stem, with buds, of the mini core-fragrant sweet potatoes, shearing the stem with buds, shearing the top buds, and cleaning and sterilizing to obtain an explant for standby; sterilizing the explant, and performing primary culture on the explant to obtain a sterile culture system; cutting the stem, with one leaf and one bud, of the mini core-fragrant sweet potato after the primary culture, inoculating the stem to a subculture medium for multiplication culture, wherein the culture temperature of the primary culture and subculture is 25-28 DEG C, and culture time is 30 days; the light intensity of the first 15 days is 1600-1800lx, and the illumination time is 12 hours every day; the light intensity of the later 15 days is 3100-3800lx, and the illumination time is 15 hours every day. The tissue culture rapid propagation method has the advantages that the sterile culture system is obtained by using the primary culture, fast multiplication of the cut stem is achieved through the subculture, multiplication times can reach 13 times, and a stable and efficient tissue culture rapid propagation technological system for the mini core-fragrant sweet potatoes is built.

Description

technical field [0001] The invention relates to the field of sweet potato detoxification, in particular to a method for tissue culture and rapid propagation of mini sweet potato. Background technique [0002] In the process of introduction, propagation and production, the new varieties or lines of Mini Xinxiang mainly use vegetative organs such as tubers and vines for asexual reproduction, so it is easy to cause the accumulation and spread of virus diseases, resulting in reduced yield, quality and seedlings. Sexual degeneration. According to statistics, the average yield reduction rate caused by sweet potato virus disease in China is 29.4%. In recent years, it has been affected by viral diseases in the process of producing mini-heart sweet potatoes, the development is slow, and the planting scale is small, which cannot meet the needs of processing enterprises. SUMMARY OF THE INVENTION [0003] The invention aims to solve the deficiencies of the prior art, and provides a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 王立国郭子江张嘉李建军朱永伶芮淑会王鸿鸣李彦阳
Owner TIANJIN FENGHUA YULONG AGRI DEV CO LTD
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