Adherent/suspension cell culture unit, device, system and method

Abstract

The invention discloses a wall adherence / suspension type cell culture unit as well as a device, a system and a method thereof and relates to the technical field of cell culture devices. The culture unit comprises a shell, wherein a plurality of cell growth plates are arranged inside the shell; the adjacent cell growth plates are alternately connected to the left side wall or the right side wall of the shell; the front side surfaces of the cell growth plates are fixed to a front side plate of the shell, and the rear side surfaces of the cell growth plates are fixed to a rear side plate of the shell; a gap is retained between the adjacent cell growth plates; a roundabout one-way flow channel is formed among the cell growth plates and the inner wall of the shell; a liquid inlet is formed in a starting position of the one-way flow channel; a liquid outlet is formed in an ending position of the one-way flow channel. The unit is simple in structure and convenient to use; the culture process is easy to monitor; the large-scale animal cell wall adherence and suspension culture can be met.

Description

technical field [0001] The invention relates to the technical field of cell culture devices, in particular to an adherent / suspension cell culture unit, device, system and method. Background technique [0002] The in vitro scale culture of cells is an efficient method for the production of biological products. In the 1960s and 1970s, the microcarrier culture system and hollow fiber cell culture technology for large-scale cell culture were established. In recent decades, due to the sharp increase in human demand for biological products such as growth hormone, interferon, monoclonal antibodies, vaccines, and interleukins, the amount of biological products obtained from animal tissues using traditional biochemical techniques has been far from being sufficient. Meet the needs. As the principles and methods of cell culture are becoming more and more perfect, the large-scale cell culture technology is gradually becoming mature. [0003] The so-called large-scale culture technolo...

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Problems solved by technology

[0006] At present, many domestic enterprises still use the spin bottle technology for production. However, due to the shortcomings of the spin bottle technology, which cannot prepare products on a large scale, the quality difference between production batches is large, and the products cannot be monitored, the state expressly prohibits the application for approval of the new spin bottle production line.

[0007] Although microcarrier bioreactors are widely used abroad, the microcarrier culture process is complicated to operate and has high requirements for cell bioreactors. Domestic equipment is completely dependent on imports.

At present, domestic enterprises cannot fully grasp the technology of microcarrier technology. Only a few enterprises can achieve the cultivation scale of 300L, and only a few enterprises in the world have mastered the cultivation technology of more than 1000L, and cannot exceed the cultivation scale of 5000L, which seriously restricts vaccines. and bioengineering drug development and production

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