4 results about "Growth plates" patented technology

This application discloses an automatic analysis method for CT images of long bones in mice and rats, relating to the field of image processing technology. The method includes: acquiring a CT image sequence of the target long bone, extracting the three-dimensional spatial distribution of bone tissue, and performing pose alignment and three-dimensional cropping to obtain a standardized bone image sequence; then constructing candidate depth intervals based on butterfly-shaped anatomical features and key points of growth plate strip-shaped gaps, respectively, and obtaining a fine search range through intersection operation; subsequently identifying candidate slices of triangular fractures and determining the anatomical zero point; finally, mapping the target analysis region based on the anatomical zero point, completing cortical bone segmentation, medullary cavity segmentation, and trabecular bone extraction under closed mask constraints, and outputting quantitative parameters of bone tissue. This application achieves accurate positioning of the growth plate and unified analysis region mapping in long bone CT images, improving the accuracy of trabecular bone extraction and the consistency of bone tissue quantification results.

The application provides a developmental growth plate layered scaffold and a preparation method and application thereof, and belongs to the technical field of bone tissue engineering materials. The developmental growth plate layered scaffold provided by the application is divided into three layers, the upper layer is a cartilage cell layer, the middle layer is a blood vessel barrier layer, and the lower layer is a bone formation layer. The blood vessel barrier layer can be used as biological glue to bond the upper layer and the lower layer, so that the composite scaffold structure is stable and is not easy to break; and the upper layer contains bone marrow mesenchymal stem cells (BMSCs), and the middle layer and the lower layer are cell-free. Under the in-vitro chondrogenic induction culture condition, the BMSCs in the upper layer can pass through the middle layer and colonize in the lower layer, and in the process, part of the BMSCs can differentiate into cartilage cells. The middle layer containing thrombospondin 1 (TSP1) has specific intervention effect on cell migration, which is manifested as inhibition of the migration of vascular endothelial cells, but has no effect on the migration of BMSCs.

The invention discloses a composition for improving functions of cartilage cells of a growth plate and promoting body growth and application of the composition, the composition comprises a first component, a second component and a third component, and the first component comprises rose acid; the second component is prepared from 3-(4-hydroxy-3-methoxyphenyl) propionic acid; the third component comprises ursolic acid and / or chitosan oligosaccharide. The components of the composition are high in safety to human bodies, the advantages of all the components can be fully combined and exerted through accurate adjustment, the functions of cartilage cells of a growth plate are better improved, and the growth of body length (height) is promoted.

The invention provides ultra-stable nano amorphous calcium phosphate and a preparation method thereof. A phosphate radical source of the amorphous calcium phosphate is adenosine triphosphate or pyrophosphate. The amorphous calcium phosphate contains alkaline phosphatase capable of hydrolyzing adenosine triphosphate, extracellular nucleotide pyrophosphatase 1 and alpha2-HS glycoprotein or secretory phosphoprotein 1 capable of stably adsorbing calcium ions. Researches prove that a mineralization raw material adenosine triphosphate involved in the mineralization process of a human body growth plate is used as a source of phosphate radicals, and protein capable of hydrolyzing adenosine triphosphate and pyrophosphate is sequentially added and combined with protein capable of adsorbing calcium ions, so that a spherical amorphous calcium phosphate cluster structure can be formed; and the formed amorphous calcium phosphate can stably exist for more than 35 days under the conditions that the pH is 7.0-7.3 and the temperature is 37 DEG C, and can stably exist for more than 120 days under the condition of 4 DEG C, so that the stabilization time of the nanoscale amorphous calcium phosphate under the mild condition is remarkably prolonged, and the method has a wide application prospect in the field of bone tissue engineering.