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Liquid containing diatom, diatom and method for culturing diatom

A cultivation method and diatom technology, which are applied in seaweed cultivation, botanical equipment and methods, cultivation and other directions, can solve the problems of inability to obtain sufficient cultivation amount and the like

Inactive Publication Date: 2009-06-17
マリンテック
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] According to the existing culture method, the upper limit of the cell concentration of Chaetoceros is about 3.0×10 13 cell / m 3 , so it is not possible to obtain a sufficient amount of culture

Method used

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  • Liquid containing diatom, diatom and method for culturing diatom
  • Liquid containing diatom, diatom and method for culturing diatom
  • Liquid containing diatom, diatom and method for culturing diatom

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0118] Culture medium 1 (1.5×10 -3 m 3 )join in Figure 7 Shown laboratory flat culture flask 11 (capacity 1.5 × 10 -3 m 3 ), add sodium bicarbonate to the culture medium 1 until the mass concentration of sodium bicarbonate is 1.0×10 3 ppm. Then, a predetermined amount of Chaetoceros was inoculated into the culture solution 1 . Then, the temperature of the culture solution is maintained at about 25° C. to about 35° C., and a fluorescent lamp or the like is used to make the 2 The culture solution 1 is irradiated with light of / s intensity, and in this state, the air mixed with 3% carbon dioxide is sent into the culture solution 1 through the glass tube 13 connecting the plug 12 of the bottle 11, and the culture solution 1 is ventilated. While stirring, the cultivation test of Chaetoceros was carried out. The time change of the Chaetoceros cell concentration and the time change of the pH in the culture solution 1 were respectively measured.

Embodiment 2

[0120] Culture medium 2 (1.5×10 -3 m 3 ) into the laboratory flat culture flask 11, adding sodium bicarbonate to a mass concentration of sodium bicarbonate is 1.0 × 10 3 ppm. Then, inoculate the chaetoceros of predetermined amount (the same amount with embodiment 1) in culture liquid 2, under the approximately identical condition (the temperature of culture liquid: about 25 ℃~about 35 ℃, photon amount: about 200μmol / m 2 / s, carried out aeration and agitation by air mixed with 3% carbon dioxide), the culture test of Chaetoceros was carried out. The time change of the Chaetoceros cell concentration and the time change of pH in the culture solution 2 were respectively measured.

Embodiment 3

[0122] Culture medium 3 (1.5×10 -3 m 3 ) into the laboratory flat culture flask 11, adding sodium bicarbonate until the mass concentration of sodium bicarbonate is 1.0 × 10 3 ppm. Then, inoculate the chaetoceros of predetermined amount (the same amount with embodiment 1) in culture liquid 3, under the approximately identical condition (culture liquid temperature: about 25 ℃~about 35 ℃, photon amount: about 200 μ mol) with embodiment 1 / m 2 / s, carried out aeration and agitation by air mixed with 3% carbon dioxide), the culture test of Chaetoceros was carried out. The time change of the Chaetoceros cell concentration and the time change of pH in the culture solution 3 were respectively measured.

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Abstract

A liquid for culturing diatom comprising nitrogen, silicon and phosphorus, wherein a value of silicon / nitrogen, representing the ratio of concentration by mass of silicon to nitrogen, is set to be a value of 0.06 or more, and a value of phosphorus / nitrogen, representing the ratio of concentration by mass of phosphorus to nitrogen, is set to be a value of 0.18 or more, and the pH of the culture liquid is set in the range of 6.4 to 8.4. The culture liquid allows the culture of Chaetoceros at a cell concentration greater than the maximum value (3 X 10<13> cells / m<3>) achieved by the conventional culture methods and with stability.

Description

technical field [0001] The present invention relates to diatoms as microalgae, which are used as bait for shellfish such as oysters and pearl oysters, crustaceans such as prawns and prawns, and aquatic seedlings such as echinoderms such as sea cucumbers and sea urchins. More specifically, it relates to A liquid containing diatoms and a method for cultivating diatoms. Background technique [0002] In recent years, diatoms classified as microalgae such as the genus Chaetoceros and the genus Phaeodactylum (such as the genus Chaetoceros) have been used as bait for breeding aquatic seedlings such as shellfish, crustaceans, and echinoderms. Calcitrans, gracilis, etc.) have begun to attract people's attention. [0003] For example, Chaetoceros calcareous has one long flagella in each of the four horns. Since the diameter (full length) of Chaetoceros calcareous is about 3 to 6 μm, it is particularly suitable as a bait for aquatic seedlings. [0004] Diatoms, one of microalgae, ar...

Claims

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Application Information

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IPC IPC(8): C12N1/12A01G33/00
CPCC12N1/12A01G33/00
Inventor 近藤裕山内一郎富田富士彦柏仓真山下卓铃木顺子
Owner マリンテック
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