Culture medium formula and full-artificial quick cultural method of Chaetoceros mulleri

A culture medium formula and the technology of Chaetoceros moowii, which are applied in the field of marine microalgae cultivation, can solve the problems of slow growth of Chaetoceros, high difficulty of aeration method, and unbalanced nutrition, so as to improve the growth rate of reproduction and increase the production efficiency. Improve and reduce the effect of gibberellin dependence

Inactive Publication Date: 2013-12-25
GUANGXI ACADEMY OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the problems of unbalanced nutrition, slow growth rate of Chaetoceros, high cost of raw materials, and high difficulty of aeration method in the existing Mou-style Chaetoceros bath culture medium and its method, and provide a culture medium formula for Chaetoceros mouii and full artificial rapid culture method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Each component and its concentration in the culture medium formula of Chaetoceros mourei in the present embodiment: urea CO(NH 2 ) 2 0.17g / L, sodium bicarbonate NaHCO 3 0.075g / L, sodium silicate crystal Na 2 SiO 3 9H 2 O 0.1g / L, potassium dihydrogen phosphate KH 2 PO 4 0.01g / L, EDTA 3~5g / L, VB 1 2mg / L, VB 12 1.5μg / L, the formulation solvent is sterilized seawater.

[0020] 18L mineral water bucket, after disinfection by potassium permanganate, rinse it twice with sterilized sea water for later use. The mineral water bucket is placed on a wooden frame, and three tricolor light sources are installed on the side of the wooden frame, so that the illuminance of the light reaching the triangular bottle or mineral water bucket is 4000lx , the seawater used for cultivation is disinfected with bleach solution, and the disinfection method is 1m 3 Add 250ml of bleach solution to the seawater to make the available chlorine content in the seawater reach 10~20mg / L. After...

Embodiment 2

[0022] Each component and its concentration in the culture medium formula of Chaetoceros mourei in the present embodiment: urea CO(NH 2 ) 2 0.1g / L, sodium bicarbonate NaHCO 3 0.05g / L, sodium silicate crystal Na 2 SiO 3 9H 2 O 0.5g / L, potassium dihydrogen phosphate KH 2 PO 4 0.02g / L, EDTA 5g / L, VB 1 5mg / L, VB 12 3μg / L, the formulation solvent is sterilized seawater.

[0023] Take a 5L glass triangular flask, disinfect it with potassium permanganate, rinse it with sterilized sea water twice for later use, place the triangular flask on a wooden frame, and set 3 tricolor light sources on the side of the wooden frame to make the light illuminance reaching the triangular flask or mineral water bucket For 5000lx, the seawater used for cultivation is disinfected with bleach solution, and the disinfection method is 1m 3 Add 250ml of bleach solution to the seawater to make the available chlorine content in the seawater reach 10~20mg / L. After 24 hours of disinfection, neutra...

Embodiment 3

[0025] Each component and its concentration in the culture medium formula of Chaetoceros mourei in the present embodiment: urea CO(NH 2 ) 2 0.3g / L, sodium bicarbonate NaHCO 3 0.1g / L, sodium silicate crystal Na 2 SiO 3 9H 2 O 0.3g / L, potassium dihydrogen phosphate KH 2 PO 4 0.02g / L, EDTA 4g / L, VB 1 1mg / L, VB 12 3μg / L, the formulation solvent is sterilized seawater.

[0026] Take an 18L mineral water bucket, disinfect it with potassium permanganate, rinse it with sterilized sea water twice for later use, place the mineral water bucket on a wooden frame, and set three tricolor light sources on the side of the wooden frame, so that the illuminance of the light reaching the triangle bottle or mineral water bucket is 3500lx, the seawater used for cultivation is disinfected with bleach solution, and the disinfection method is 1m 3 Add 250ml of bleach solution to the seawater to make the available chlorine content in the seawater reach 10~20mg / L. After 24 hours of disinfe...

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Abstract

The invention belongs to the field of microalgae cultivation, and relates to a culture medium formula and a full-artificial quick cultural method of Chaetoceros mulleri. The culture medium formula comprises 0.1-0.3g/L of urea, 0.05-0.1g/L of sodium hydrogen carbonate, 0.1-0.5g/L of sodium silicate hydrate crystals, 0.01-0.02g/L of potassium dihydrogen phosphate, 3-5g/L of EDTA (Ethylene Diamine Tetraacetic Acid), 1-5mg/L of VB1 and 1.0-3 microgram/L of VB12, wherein the solvent of the formula is seawater. The cultural method comprises the following steps: after disinfecting a culture tank and seawater, preparing a culture medium according to the formula by using seawater as the solvent; inoculating the algae to the culture tank filled by the culture medium; cultivating after sealing; illuminating by light with three primary colors and inflating air which is filtered by an air filter element with the aperture which is less than 0.1 micron during cultivation; and cultivating for 3-5 days and collecting. The culture medium formula provided by the invention is balanced in nutrition and low in cost; according to the cultural method, the chaetoceros mulleri grows quickly and the method is simple in process operation.

Description

technical field [0001] The invention belongs to the field of marine microalgae cultivation, and specifically relates to a culture medium formula of Chaetoceros mouroii and a method for rapid artificial cultivation. Background technique [0002] The cells of Chaetoceros moowii are small and the cell walls are thin. Most of the single cells, but also 2-3 cells connected into groups. The shell surface is oval to round, and the center is slightly convex or a few flat. The annulus of the shell is rectangular to quadrangular, and the general cell size is usually 3.45-4.6 microns wide and 4.6-9.2 microns long in the annular view, and the shell annulus is not obvious. Horn capillaries are thin and long, with pointed ends, arising from the four corners of the cells, almost parallel to the longitudinal axis, generally 20.7-34.5 microns long. Viewed from the shell surface, the horn hairs at both ends are slightly "S" shaped with the cell body as the center. One pigment body, in fla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 杨彦豪邓潜黎铭陈晓汉蒋伟明杨春玲彭金霞韦嫔媛
Owner GUANGXI ACADEMY OF FISHERY SCI
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