Chaetoceros minutissismus concentrated culture solution and culture method for culturing Chaetoceros minutissismus

Abstract

The invention discloses a preparation method of a Chaetoceros minutissismus concentrated culture solution and high-efficiency culture technology, belonging to the aquaculture field. The prepared Chaetoceros minutissismus concentrated culture solution can be stored and transported conveniently and can be produced conveniently in a factory-like, standard and specialty manner, microconstituents can be quantified accurately, and a traditional f / 2 nutritive salt formula is optimized, and the constituents are reasonable and balanced and are beneficial to the rapid growth of the microalgae; a plastic purified water barrel is used for breeding Chaetoceros minutissismus, the operation is flexible, pollution cannot be caused easily, the washing operation is convenient, and the plastic purified water barrel cannot be scratched easily, and is low in cost; different illuminations and temperatures are provided in early, middle and later culture periods, nutritive salt is supplemented in the later culture period to promote the continuous and rapid growth of the Chaetoceros minutissismus, and the algal yield is improved by 120%; and three plastic barrels are connected in series for breeding, introduced gas is utilized for three times, and thus energy and resources are saved.

Description

[0001] Technical field The present invention belongs to the field of aquaculture, and in particular relates to a method for preparing a concentrated culture solution of Chaetoceros miniature and a high-efficiency culture technology. Background technique [0002] Chaetoceros minutissismus is a small marine planktonic diatom belonging to Diatomphyta, Central Class, Boxophyceae, Chaetoceros family, and Chaetoceros genus. The cells are small, with thin walls, mostly single cells, and 2-3 cells are also connected into groups. The shell surface is oval to round, slightly raised in the center, and a few are flat. The annulus of the shell is rectangular to quadrangular, the cells are 4.6-9.2 microns long and 3.45-4.6 microns wide, and the shell annulus is not obvious. Horn capillaries are slender and long, with pointed ends, emanating from the four corners of the cells, almost parallel to the longitudinal axis, 20.7-34.5 microns long. The horn hairs at both ends are slightly "S" sha...

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Problems solved by technology

The medium formula of the invention is nutritionally balanced, low in cost, and the cultivation method enables Chaetoceros to grow rapidly, and the process is simple to operate, but the volume of the cultivation tank is small, and only a small amount of algal liquid can be cultivated at a time, so it is difficult to popularize and apply it in production, and the cultivation tank must be purchased. Large investment, high technical requirements, difficult for ordinary workers to master

Yang Hailin invented a high-density culture process for autotrophic oil-producing microalgae (application number CN201010509643), which uses a fermentation method coupled with a segmentation method and a two-step method, and uses CO emitted by thermal power plants 2 Exhaust gas is used as a carbon source, and municipal sewage after filtering out miscellaneous bacteria or miscellaneous algae is used as a medium to cultivate oil-producing microalgae at high density. , Sea green coccus, isochrysis globulus, Nitzschia crescentus, Phaeodactylum tricornutum, Dunaliella and Nitztella, the invention can realize nitrogen, phosphorus and CO 2 High removal efficiency and high algae concentration, oil and protein content, to achieve the dual effects of protecting the environment and producing biological resources, low cost, no pollution, high comprehensive benefits, but the process is long, troublesome to operate, easy to pollute, and low in efficiency

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