Macro-element nutrient salt formula for large-scale culture of Chaetoceros mulleri

A technique for large-scale cultivation of Chaetoceros moubii, applied in the field of macronutrient nutrient salt formula and its preparation, capable of solving problems affecting seedling cultivation process and seedling quality, unstable nutrient composition of cells, failure to solve the problem of Chaetoceros mouvii, etc. problem, to achieve the effect of promoting heterogeneous reaction rate, promoting good metabolism, and cultivating for a long time

Inactive Publication Date: 2015-12-30
ZHANJIANG HENGXING SOUTHERN OCEAN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

F/2 medium has disadvantages such as easy contamination and low culture density, which can easily lead to low culture efficiency and unstable nutrient composition of the culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1 adopts the following steps to realize the present invention:

[0021] 1. Formula: Mother liquor A is composed of 20 parts by weight of ammonium bicarbonate, 1 part by weight of sodium dihydrogen phosphate, 0.39 parts by weight of ferric chloride, 1.64 parts by weight of disodium edetate, and the rest of water; Mother liquor B is made up of sodium silicate, water of 15 parts by weight, and the preparation of described substratum comprises the steps:

[0022] (1) Preparation of mother liquor A: Weigh ammonium bicarbonate, sodium dihydrogen phosphate, ferric chloride, disodium edetate and water according to the formula of mother liquor A, and mix ammonium bicarbonate, sodium dihydrogen phosphate , Disodium EDTA was dried at 50~65°C, mixed well, dissolved in two-thirds of hot water to obtain a mixed solution, and then ferric chloride was dissolved in the remaining one-third In 1 liter of hot water, ferric chloride solution is obtained, the ferric chloride solu...

Embodiment 2

[0030] Embodiment 2 adopts the following steps to realize the present invention:

[0031] 1. Formula: Mother liquor A is composed of 23 parts by weight of ammonium bicarbonate, 1.2 parts by weight of sodium dihydrogen phosphate, 0.39 parts by weight of ferric chloride, 1.64 parts by weight of disodium edetate, and the remainder of water; Mother liquor B is made up of the sodium silicate of 18 weight parts, the water of 1000 weight parts, and the preparation of described substratum comprises the steps:

[0032] (1) Preparation of mother liquor A: Weigh ammonium bicarbonate, sodium dihydrogen phosphate, ferric chloride, disodium edetate and water according to the formula of mother liquor A, and mix ammonium bicarbonate, sodium dihydrogen phosphate , Disodium EDTA was dried at 50~65°C, mixed well, dissolved in two-thirds of hot water to obtain a mixed solution, and then ferric chloride was dissolved in the remaining one-third In 1 liter of hot water, ferric chloride solution is ...

Embodiment 3

[0040] Embodiment 3 adopts the following steps to realize the present invention:

[0041] 1. Formula: Mother liquor A is composed of 25 parts by weight of ammonium bicarbonate, 2 parts by weight of sodium dihydrogen phosphate, 0.39 parts by weight of ferric chloride, 1.64 parts by weight of disodium edetate, and the rest of water; Mother liquor B is made up of sodium silicate, water of 20 weight parts, and the preparation of described substratum comprises the steps:

[0042] (1) Preparation of mother liquor A: Weigh ammonium bicarbonate, sodium dihydrogen phosphate, ferric chloride, disodium edetate and water according to the formula of mother liquor A, and mix ammonium bicarbonate, sodium dihydrogen phosphate , Disodium EDTA was dried at 50~65°C, mixed well, dissolved in two-thirds of hot water to obtain a mixed solution, and then ferric chloride was dissolved in the remaining one-third In 1 liter of hot water, ferric chloride solution is obtained, the ferric chloride soluti...

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PUM

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Abstract

The invention discloses a macro-element nutrient salt culture medium for large-scale culture of Chaetoceros mulleri and a preparation method thereof. The macro-element formula culture medium is prepared by using mother liquor A and mother liquor B through mixing according to volume ratio 1:1, wherein the mother liquor A is mother liquor A (1000x) consisting of 20 to 25 parts of ammonium hydrogen carbonate by weight, 1 to 2 parts of sodium dihydrogen phosphate by weight, 0.33 part of ferric trichloride by weight, 1.64 parts of EDTA-2Na and balance of water; the mother liquor B consists of 15 to 20 parts of sodium silicate by weight and 1000 parts of water by weight. By adopting the macro-element nutrient salt formula provided by the invention to culture the Chaetoceros mulleri, the growth of the Chaetoceros mulleri can be promoted, the culture time is prolonged, the high-density culture of the Chaetoceros mulleri is realized and the culture cost is reduced.

Description

technical field [0001] The invention belongs to the field of aquaculture, in particular to a formula of macroelement nutrient salt suitable for large-scale cultivation of Chaetoceros mouroii and a preparation method thereof. Background technique [0002] As a renewable biomass energy source, microalgae has also received more and more attention. There are also related researches on the industrialization of large-scale cultured microalgae and some progress has been made. Due to the influence of various factors, the existing microalgae industrialization model still has the disadvantages of low efficiency and high cost. [0003] Chaetoceros moubii is a kind of microalgae, which belongs to Diatomphyta, Centrophyta, Boxyphyta, Chaetoceroceae, and Chaetoceros. The cells are small, mostly 5-10 microns, most of them are It is distributed in single cells, and sometimes 2~3 cells form a chain. It is a high temperature resistant species and is suitable for cultivation in summer. Chaet...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 李色东刘涛吴悦
Owner ZHANJIANG HENGXING SOUTHERN OCEAN TECH
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