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Method for rapidly propagating bait microalgae biomass by utilizing polyculture

A bait microalgae and culture medium technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of slow cell growth, long generation time, inability to effectively improve the growth of commonly used bait microalgae, etc. The effect of improving nutritional value, reducing production costs and increasing the speed of reproduction

Active Publication Date: 2021-03-30
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the slow cell growth and long generation time of these bait algae in the actual breeding process are not enough to meet the requirements of rapid growth of shellfish.
However, the current conventional methods for improving the bioproductivity of microalgae, such as optimization of culture conditions and improvement of culture devices, cannot effectively improve the growth of commonly used bait microalgae.

Method used

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  • Method for rapidly propagating bait microalgae biomass by utilizing polyculture
  • Method for rapidly propagating bait microalgae biomass by utilizing polyculture
  • Method for rapidly propagating bait microalgae biomass by utilizing polyculture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the cultivation effect comparison of different organic carbon sources of bait microalgae

[0020] Three commonly used bait microalgae Chlorella, Thalassiosira pseudonana, and Chaetoceros were placed in f / 2 medium, and the light intensity was 80 μmol.m -2 .s -1 , under the condition of temperature 25°C, the liquid mixed nutrient culture in the triangular flask was carried out ( figure 1 ), and f / 2 medium were added with a concentration of 2.0g.L -1 The organic carbon sources glucose, acetic acid and glycerol. The group distribution ratio of f / 2 medium is as follows (mg.L -1 ): Sodium Nitrate 75, Sodium Dihydrogen Phosphate Monohydrate 5, Sodium Silicate Nonahydrate 30, Ferric Chloride Hexahydrate 3.15, Disodium EDTA Dihydrate 4.36, Copper Sulfate Pentahydrate 0.0098, Sodium Molybdate Dihydrate 0.0063, zinc sulfate heptahydrate 0.022, cobalt chloride hexahydrate 0.01, manganese chloride tetrahydrate 0.18, vitamin B 1 0.1, vitamin H 0.0005, vitamin B 1...

Embodiment 2

[0022] Example 2: Optimal screening of different carbon source concentrations under mixed nutrition conditions

[0023] according to figure 1 The experimental program, three bait microalgae Chlorella, Thalassiosira pseudonana, Chaetoceros under the same light intensity (80μmol.m -2 .s -1 ) and temperature conditions (25°C); add organic carbon sources acetic acid, glycerol, and glycerol to f / 2 normal medium, respectively. The concentrations of the three organic carbon sources are set to 0.5, 1.0, 2.0, 5.0g.L respectively -1 , after cultivating respectively 11 days under the same conditions as in Example 1, the result is as follows image 3 shown. The results showed that Chlorella in the concentration of acetic acid 5.0g.L -1 The growth effect is good, and the biomass is 141.7×10 5 cells / ml, better than the value of f / 2 normal medium 84×10 5 cells / ml; Thalassiosira pseudonana in the organic carbon source glycerol concentration 5.0g.L -1 The lower growth is good, the biom...

Embodiment 3

[0024] Example 3: Algae cell biochemical components and main fatty acid changes under optimal organic carbon source and concentration of bait microalgae

[0025] With reference to the conditions described in Example 1 and Example 2, 3 strains of bait microalgae Chlorella, Thalassiosira pseudonana, and Chaetoceros were respectively added with an optimal organic carbon source concentration of 5.0g.L -1 Acetic acid, 5.0g.L -1 Glycerin and 5.0g.L -1 Glycerol was cultured in f / 2 normal medium for 11 days, and then the content of oil, sugar, protein components and main fatty acids of algae cells were determined respectively. The result is as Figure 4 As shown, the polysaccharide and lipid components of the three bait algae under the condition of organic carbon source were all better than those under the corresponding normal medium condition.

[0026] Most importantly, under the dominant organic carbon source and concentration of Thalassiosira pseudonana, the component contents o...

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Abstract

The invention provides a culture method for rapid propagation of bait microalgae. The culture method comprises the following steps: adding an organic carbon source into an f / 2 culture medium to culture the bait microalgae; wherein the bait microalgae are pseudominiature sea chain algae, chlorella or chaetoceros respectively; preferably, the organic carbon source added into the f / 2 culture medium is glycerol, acetic acid or glucose, wherein the addition concentration of the organic carbon source is 0.5-5.0 g / L. According to the method, the nutritional value of the bait microalgae can be effectively improved, the propagation speed of the bait microalgae can be increased, the production cost of the bait microalgae is reduced, the economy of shellfish culture is improved, and technical supportis provided for wide and low-cost application of microalgae shellfish baits.

Description

technical field [0001] The invention belongs to the technical field of bait microalgae expansion and cultivation and the production of active products thereof, and in particular relates to a method for quickly multiplying bait microalgae biomass by using polyculture culture. Background technique [0002] Bivalve molluscs are an important economic pillar of the marine biological industry and account for a significant share of world fishery production. Live microalgae have been used in the bivalve mollusc culture process and are considered to be the most suitable feed for the growth of shellfish larvae and juveniles. Microalgae are the bottom organisms of the food chain in water ecosystems. Some algae cells are rich in oils, polysaccharides and polyunsaturated fatty acids, which have high nutritional value and are the key to improving the survival rate of bivalve mollusk seedlings. [0003] At present, some bait microalgae, such as Thalassiosira pseudonana, Chlorella, Chaetoc...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12Y02A40/81
Inventor 程鹏飞褚蕊蕊周成旭严小军徐继林
Owner NINGBO UNIV
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