Simulation three-dimensional cell cultivation device and cultivation method

Abstract

The invention discloses a simulation three-dimensional cell cultivation device and a cultivation method. The simulation three-dimensional cell cultivation device highly simulates the in-vivo tumor physiological environment, and can be used for detecting and screening effects of novel anti-tumor medicines or detecting sensitivity of tumor cells on different anti-tumor medicines, factors such as nutrition supply of the tumor cells, removal of metabolic wastes, blood vessels and vascular endothelial cells are considered, and the in-vivo tumor physiological environment is close to the in-vivo tumor growth environment. By the invention, the in-vitro tumor cell physiological environment is greatly close to the in-vivo environment; and by the new technology, detection and screening of the novel anti-tumor medicines and tumor cell medicine sensitive tests are improved to a new height.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a simulated three-dimensional cell culture device and a culture method. Background technique [0002] Tumor tissue in the human body is a cell population composed of tumor cells, stromal cells (including fibroblasts, inflammatory and immune cells), blood vessels, and extracellular matrix. The microenvironment in which tumor cells grow has an important impact on tumor growth. The traditional cell culture technology is to plant tumor cells in a vessel filled with cell culture fluid. Using this traditional cell culture technology, tumor cells are either suspended in the culture medium or attached to the walls of the culture dish, without forming a three-dimensional structure similar to tumors in vivo. The three-dimensional cell culture developed subsequently is to mix tumor cells or tumor tissue digested fragments with extracellular matrix, and then place them in cell culture medium for...

AI Technical Summary

Problems solved by technology

However, these three-dimensional cell culture techniques are still very different from tumors in vivo because they do not take into account the factors of tumor cell nutrition supply, metabolic waste removal, blood vessels and vascular endothelial cells.

Images