Simulation three-dimensional cell cultivation device and cultivation method

A three-dimensional cell and cell culture technology, applied in the field of cell culture, can solve the problems of tumor difference, nutrient supply and metabolic waste removal of tumor cells, etc. are not considered.

Active Publication Date: 2012-04-04
佛山市康伲爱伦生物技术有限公司
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Problems solved by technology

However, these three-dimensional cell culture techniques are still very different from tumors in vivo because they do not take

Method used

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  • Simulation three-dimensional cell cultivation device and cultivation method
  • Simulation three-dimensional cell cultivation device and cultivation method
  • Simulation three-dimensional cell cultivation device and cultivation method

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Embodiment Construction

[0018] The present invention is further explained below in conjunction with the examples, but the examples do not limit the present invention in any form.

[0019] The inner container and the outer container are made of organic materials suitable for cell culture. The device can be composed of a single inner container and outer container or a series of inner containers and outer containers arranged vertically and horizontally.

[0020] The osmotic support membrane is made of organic materials suitable for cell culture, and the membrane has dense pores for the passage of culture fluid and various organic and inorganic components. The pore diameter is much smaller than the cell diameter, so the vascular endothelial cells do not fall off and can form a vascular endothelial cell layer on the membrane.

[0021] Tumor cells and various stromal cells can be isolated from tumor tissues or purchased from commercially available cell lines.

[0022] Extracellular matrix can be extracte...

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Abstract

The invention discloses a simulation three-dimensional cell cultivation device and a cultivation method. The simulation three-dimensional cell cultivation device highly simulates the in-vivo tumor physiological environment, and can be used for detecting and screening effects of novel anti-tumor medicines or detecting sensitivity of tumor cells on different anti-tumor medicines, factors such as nutrition supply of the tumor cells, removal of metabolic wastes, blood vessels and vascular endothelial cells are considered, and the in-vivo tumor physiological environment is close to the in-vivo tumor growth environment. By the invention, the in-vitro tumor cell physiological environment is greatly close to the in-vivo environment; and by the new technology, detection and screening of the novel anti-tumor medicines and tumor cell medicine sensitive tests are improved to a new height.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a simulated three-dimensional cell culture device and a culture method. Background technique [0002] Tumor tissue in the human body is a cell population composed of tumor cells, stromal cells (including fibroblasts, inflammatory and immune cells), blood vessels, and extracellular matrix. The microenvironment in which tumor cells grow has an important impact on tumor growth. The traditional cell culture technology is to plant tumor cells in a vessel filled with cell culture fluid. Using this traditional cell culture technology, tumor cells are either suspended in the culture medium or attached to the walls of the culture dish, without forming a three-dimensional structure similar to tumors in vivo. The three-dimensional cell culture developed subsequently is to mix tumor cells or tumor tissue digested fragments with extracellular matrix, and then place them in cell culture medium for...

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Application Information

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IPC IPC(8): C12M3/00C12N5/09C12Q1/02
CPCC12M25/02C12M23/34C12M23/08C12M29/04
Inventor 郑希杜志云艾伦.康尼张焜苏博云
Owner 佛山市康伲爱伦生物技术有限公司
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