Micro-valve-based dual-detection microfluidic chip and diabetic model construction method

A microfluidic chip and dual-detection technology, applied in the field of microfluidic chip and diabetes research, to achieve the effect of simple method, simplified chip structure and high throughput

Inactive Publication Date: 2016-06-29
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

[0003] As a disease that endangers human health, diabetes has attracted more and more attention. Caenorhabditis elegans, as a model organism, has become a hot spot in diabetes research due to the conservation of insulin signaling pathway genes. However, existing technologies Difficult to perform relevant diabetes research on single nematodes

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  • Micro-valve-based dual-detection microfluidic chip and diabetic model construction method
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  • Micro-valve-based dual-detection microfluidic chip and diabetic model construction method

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Embodiment 1

[0035] The microfluidic chip designed and produced by the laboratory: the material for the preparation of the microvalve-based dual-detection microfluidic chip is PDMS polymer, the seal between the liquid circuit and the gas circuit is reversible, and the gas circuit and the glass sheet The sealing between them adopts plasma irreversible sealing.

[0036] The chip consists of three layers, the upper layer is the liquid circuit layer, the middle layer is the PDMS membrane, and the lower layer is the gas circuit control layer.

[0037] Wherein the liquid path layer is made up of 30 nematode analysis functional units, all functional units share a central waste liquid pool 6; each analysis functional unit consists of worm and drug inlet 1, sampling channel 2, nematode fixation and culture chamber 3 (dimensions: 2mm×1mm×80μm), waste liquid channel 4 components ( figure 2 ); the worm and drug inlet 1 is connected with the nematode fixation and culture chamber 3 through the samplin...

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Abstract

The invention provides a microvalve-based dual-detection microfluidic chip and a method for constructing a diabetes model. The chip is composed of three layers of PDMS, the upper layer is the liquid circuit layer, the lower layer is the air circuit control layer, and the middle of the upper and lower layers is the PDMS membrane. A method for constructing a diabetes model based on a microvalve-based dual-detection microfluidic chip. A single nematode is added to the entrance of the worm and the drug, and the single worm is introduced into the nematode fixation and culture room. During fluorescence imaging, the air pressure is used to deform the PDMS membrane, and the worm body is compressed. It is fixed and imaged on the side wall of the sampling channel; material exchange is performed three times a day to add fresh food and apply glucose or drug stimulation to discharge nematode metabolic waste. The invention adopts channel design, which is simple to operate and easy to realize material exchange, which is beneficial to the long-term cultivation of nematodes; the method of fixing nematodes adopts the method of membrane deformation, which is safe and harmless to the nematodes, and can be used for long-term cultivation, movement and imaging of a single nematode. detection.

Description

technical field [0001] The invention mainly relates to a microfluidic chip and diabetes research, a microvalve-based double-detection microfluidic chip and a method for constructing a diabetes model. Background technique [0002] In the prior art, a microfluidic chip, also known as a labonachip, refers to the integration or basic integration of basic operating units such as sample preparation, reaction, separation, and detection involved in the fields of biology and chemistry. On a chip of a few square centimeters (or even smaller), a network of microchannels is formed, and a controllable fluid runs through the entire system to replace various functions of conventional biological or chemical laboratories. Its basic feature and greatest advantage are the flexible combination and large-scale integration of various unit technologies on a tiny platform. So far, microfluidic chip technology has begun to be applied in different fields of life sciences, and has become one of the i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 秦建华朱国丽尹方超
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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