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Method and device for preparing electrospinning fiber bracket with three-dimensional structure

A technology of electrospun fibers and three-dimensional structures, which is applied in the direction of scaffolds, fiber processing, and complete sets of equipment for the production of artificial threads. value effect

Inactive Publication Date: 2012-02-22
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the method for preparing fibrous tissue engineering scaffolds with three-dimensional structure is relatively complicated, so further improvement is needed

Method used

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  • Method and device for preparing electrospinning fiber bracket with three-dimensional structure
  • Method and device for preparing electrospinning fiber bracket with three-dimensional structure
  • Method and device for preparing electrospinning fiber bracket with three-dimensional structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: A preparation device for electrospun fiber scaffold with three-dimensional structure

[0044] This embodiment provides a preparation device for an electrospun fiber scaffold with a three-dimensional structure such as figure 1 As shown, it consists of a spinning cabinet (1), a high-voltage power supply (2), a micro propulsion pump (3), a collector (4) and a rotating device (5); the top of the spinning cabinet (1) is equipped with a micro propulsion pump (3), the micro propulsion pump (3) is connected with a syringe (6); the interior of the spinning cabinet is provided with a collector (4) and a rotating device (5), and the collector (4) is an insulated bowl on the inner wall of the upper opening Type container, divide the bowl container into 5 equal parts with steel needle (16) along the central axis of the bowl container (see figure 2 ), the steel needles on the junction of any two adjacent parts are distributed at the same height, and three steel needles...

Embodiment 2

[0045] Example 2 : Using PLLA as a raw material to prepare three-dimensional nanofibrous tissue engineering scaffolds

[0046] 1. Preparation of spinning solution: Weigh 60 mg of PLLA (molecular weight = 200,000 Daltons) and dissolve in 1 ml (9:1, CH 2 Cl 2 / DMF, v / v) solvent, prepared 6% PLLA solution, sealed with parafilm, magnetically stirred for 3 hours, and set aside.

[0047] 2. Spinning process: adjust the parameters of the micro propulsion pump, the capacity is 0.2 ml, the propulsion speed is 0.5 ml / hour, and it is running. Turn on the rotating device and adjust the speed to make the rotation stable. Turn on the high-voltage power supply, adjust the voltage value to 14 kV, and start spinning.

[0048] 3. Separation of spinning fibers: Use a glass rod to gently remove the prepared three-dimensional fibers along the direction of the collector bowl wall. During this process, the force should be moderate to prevent the fiber material from sticking and destroying its t...

Embodiment 3

[0050] Example 3: Preparation of three-dimensional nanofibrous tissue engineering scaffolds using PCL as raw material

[0051] 1. Preparation of spinning solution: Weigh 60 mg of PCL (molecular weight = 80,000 Daltons) and dissolve in 1 ml (9:1, CH 2 Cl 2 / DMF, v / v) solvent to prepare a 6% PCL solution, seal the gap between the bottle cap and the bottle body of the strain bottle with a parafilm, stir magnetically for about 3 hours, and set aside.

[0052] 2. Spinning process: adjust the parameters of the micro propulsion pump, the capacity is 0.2 ml, the propulsion speed is 0.5 ml / hour, and it is running. Turn on the rotating device and adjust the speed to make the rotation stable. Turn on the high-voltage power supply, adjust the voltage value to 14 kV, and start spinning.

[0053] 3. Separation of spinning fibers: Use a glass rod to gently remove the prepared three-dimensional fibers along the direction of the collector bowl wall. During this process, the force should b...

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Abstract

The invention discloses a method and device for preparing an electrospinning fiber bracket with a three-dimensional structure. The preparation device can be used for generating a body form electric field and directly preparing a three-dimensional electrospinning fiber bracket in the electrostatic spinning process. The preparation method has a simple process and low cost, and is suitable for preparing three-dimensional electrospinning fibers of different chemical strengths, biocompatibilities and degradation properties by using different spinning materials. The three-dimensional electrospinning fiber bracket has the advantages of capability of allowing seed cells enter the bracket smoothly, realization of in-vitro three-dimensional culturing and tissue engineering repair of the seed cells, contribution to the adhesion growth of the cells, conveying of nutrient substances and discharge of metabolic wastes, facilitation of the proliferation, growth and differentiation of the cells in the bracket, increase in the survival rate and breeding speed of the cells, establishment of a good metabolic environment for the growth and proliferation of the cells, contribution to the breeding of the cells and healing of tissues, suitability for cell three-dimensional culturing and large application value in the field of tissue engineering.

Description

technical field [0001] The invention belongs to the field of tissue engineering scaffold systems, and in particular relates to a method for preparing an electrospun fiber scaffold with a three-dimensional structure and a preparation device thereof. Background technique [0002] Tissue engineering is playing an increasingly important role in the repair of damaged tissues and organs in the human body. At present, there are a large number of clinically applied products in the repair of some simple tissue injuries such as skin, bone, and cartilage. Tissue engineering is one of the main directions of modern biomedical engineering development. The method of tissue engineering is to combine the cells with the scaffold, promote the proliferation and differentiation of the seed cells while degrading the scaffold material, promote the organization of the seed cells, and realize the repair of damaged tissues and organs. Therefore, the scaffold material is one of the most critical fact...

Claims

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Application Information

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IPC IPC(8): D01D5/00D01D13/00A61F2/82
Inventor 冯章启朱美灵龚逸鸿蒋庆靳林何帆
Owner SUN YAT SEN UNIV
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