Rhodiola rosea oral liquid for removing astringent taste and preparation method thereof

Abstract

The invention discloses astringent-taste-removing rhodiola rosea oral liquid and a preparation method thereof, and belongs to the technical field of traditional Chinese medicinal material preparations, the astringent-taste-removing rhodiola rosea oral liquid is prepared from rhodiola crenulata, radix glehniae, fructus lycii, angelica sinensis and honey by removing tannin through a calcium hydroxide precipitation method; according to the preparation method of the rhodiola rosea oral liquid, the rhodiola rosea is in synergistic compatibility with traditional Chinese medicinal materials such as radix glehniae, fructus lycii and radix angelicae sinensis, so that the rhodiola rosea has a complementary effect in efficacy, the original bitter taste of the rhodiola rosea is remarkably improved through formula optimization, and the taking palatability and clinical adaptability of the preparation are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of Chinese medicinal material preparations, and in particular to a rhodiola rosea oral liquid with astringency-removed and a preparation method thereof. Background Art

[0002] Rhodiola rosea is a medicinal herb with anti-ischemic, anti-hypoxic, anti-fatigue, and athletic performance-enhancing effects. Its main active ingredient is salidroside, but it also contains a large amount of tannins. Tannins can cause severe astringency and poor clarity in oral solutions, affecting palatability. Current methods for removing tannins include alcohol precipitation, gelatin flocculation, and freezing, but these methods suffer from difficulties in filtration, significant loss of active ingredients, and poor taste. Therefore, a preparation method that can efficiently remove tannins, retain active ingredients, and improve mouthfeel is urgently needed. Summary of the Invention

[0003] The purpose of the present invention is to provide a rhodiola rosea oral liquid with astringency-removing properties and a preparation method thereof, so as to solve the problems in the prior art of rhodiola rosea oral liquid caused by the presence of tannins, such as severe astringency, poor clarity, low retention rate of active ingredients and complex preparation process.

[0004] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0005] The invention discloses a rhodiola rosea oral liquid for removing astringency, and the raw materials thereof include rhodiola rosea, Glehnia littoralis, wolfberry, angelica sinensis and honey.

[0006] A further technical solution is that the Rhodiola rosea oral liquid includes the following components: 1800-2200 parts of Rhodiola rosea, 2100-2700 parts of Glehnia littoralis, 2900-3300 parts of wolfberry, 2600-3100 parts of angelica sinensis, and 1700-2200 parts of honey.

[0007] A further technical solution is that the formula is calculated based on 1000 bottles, and the Rhodiola rosea oral liquid includes the following components:

[0008] 2000 parts of Rhodiola rosea, 2500 parts of Adenophora adenophora, 3000 parts of Lycium barbarum, 3000 parts of Angelica sinensis, and 2000 parts of honey.

[0009] Another object of the present invention is to provide a method for preparing astringent Rhodiola rosea oral liquid, comprising the following steps:

[0010] 1) crushing the Rhodiola rosea medicinal material, extracting it with ethanol under reflux at 60-85° C. for multiple extractions, each for 1 hour, combining the extracts, filtering, and concentrating to recover the ethanol to obtain a Rhodiola rosea extract concentrate; diluting the concentrate with water to 1.2 times the weight of the Rhodiola rosea medicinal material, adding a calcium hydroxide suspension to adjust the pH to 10-11, allowing the mixture to settle, filtering to obtain a filtrate, and then adjusting the pH of the filtrate to 6-7 with a 5% citric acid aqueous solution to obtain a Rhodiola rosea extract for removing astringency;

[0011] 2) Crush the Radix Adenophorae, Lycium barbarum, and Angelica sinensis herbs, add 10 times the weight of the herbs to the decoction, and boil for 30 minutes at 90-100°C multiple times. Combine the extracts, filter, and concentrate to 1.5 times the weight of the original herbs. Refrigerate and centrifuge to remove impurities to obtain a clear Chinese herbal extract.

[0012] 3) The Rhodiola rosea extract from step 1) and the Chinese herbal extract from step 2) are evenly mixed, refined honey prepared from a prescribed amount of honey is added, and the volume is adjusted to the target volume with purified water. After filtering, the mixture is packaged into 20 ml bottles of oral solution, sterilized by moist heat at 121°C for 30 minutes, and packaged to obtain the finished product after passing the light inspection.

[0013] A further technical solution is that the ethanol reflux extraction is performed twice in step 1).

[0014] A further technical solution is that the ethanol concentration in step 1) is 65%.

[0015] A further technical solution is that the concentration of calcium hydroxide in step 1) is 10%.

[0016] A further technical solution is that the static sedimentation in step 1) is not less than 24 hours.

[0017] A further technical solution is that the refrigeration in step 2) is not less than 24 hours.

[0018] A further technical solution is that the purified water decoction extraction is performed twice in step 2).

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] 1. In the preparation method of the Rhodiola rosea oral liquid of the present invention, Rhodiola rosea is synergistically combined with Chinese medicinal materials such as Northern Adenophora, Lycium barbarum, and Angelica sinensis, which not only has a complementary effect on the pharmacological efficacy, but also significantly improves the original bitter taste of Rhodiola rosea through formula optimization, thereby improving the palatability and clinical adaptability of the preparation.

[0021] 2. Different from the prior art method of directly adding precipitants to remove astringency, the present invention adopts a method of alkaline pH adjustment precipitation combined with acidic adjustment rotation to effectively remove the bitter tannin components in Rhodiola rosea. While improving the taste, it retains the active ingredients and ensures the stability and efficacy of the preparation.

[0022] 3. The present invention adopts a multi-stage treatment method including ethanol reflux extraction and temperature-controlled concentration, refrigerated sedimentation, centrifugal purification, honey refining and compounding, double filtration and wet heat sterilization in the preparation process. The overall process is gentle and controllable, avoiding high temperature destruction of effective ingredients, improving extraction efficiency and liquid clarity, and ensuring that the physical and chemical indicators of the product meet the standards.

[0023] 4. The preparation method of the present invention has clear process steps, simple operation, and an operating process that is suitable for the standards of modern Chinese medicine preparation production lines. It has good adaptability to large-scale, continuous, and high-efficiency production, and is conducive to the large-scale, low-cost industrial production and promotion of Rhodiola rosea oral liquid. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0025] Example:

[0026] A rhodiola rosea oral liquid for removing astringency comprises the following components:

[0027] Formula (taking the preparation of 1000 oral liquids as an example):

[0028] Rhodiola rosea medicinal material: 2000 grams

[0029] Radix Adenophorae: 2500g

[0030] Ningxia wolfberry medicinal material: 3000 grams

[0031] Angelica: 3000g

[0032] Honey (refined honey): 2000 grams

[0033] Purified water: appropriate amount

[0034] A method for preparing a rhodiola rosea oral liquid with astringency removal comprises the following steps:

[0035] Step 1): Crush the Rhodiola rosea medicinal material with a crusher, reflux extract with 65% ethanol 10 times the weight of the medicinal material twice, each time for 1 hour, the temperature is 60-85 ° C, the extract is filtered through a 300-mesh filter, concentrated and recovered ethanol, the concentrate is diluted with appropriate amount of water to 1.2 times the weight of the Rhodiola rosea medicinal material, add appropriate amount of 10% calcium hydroxide suspension to adjust the pH to between 10-11, precipitate for 24 hours, filter through a cartridge filter (0.5 micron filter element), and adjust the filtrate to between 6-7 with 5% citric acid for standby use.

[0036] Step 2): Crush the Radix Adenophorae, Lycium barbarum, and Angelica sinensis with a crusher, boil and extract twice with purified water 10 times the weight of the medicinal materials, each time for 30 minutes, at a temperature of 90-100°C, filter the extract through a 300-mesh filter, and concentrate to 1.5 times the weight of the medicinal materials. Refrigerate for 24 hours, remove impurities with a centrifuge, and set aside.

[0037] Step 3): Combine the two parts and mix them evenly, add the refined honey made from the formula amount of honey and mix well, dilute to the specified volume with purified water (20 liters per 1,000 bottles), filter once through a cartridge filter, and fill the filtrate into 20 ml oral liquid bottles, sterilize with moist heat (121°C, 30 minutes), inspect with a light, and package.

[0038] Comparative Example 1:

[0039] The Rhodiola rosea extract from step 1) of the above example was concentrated to a relative density of 1.05-1.08. 95% ethanol was added while stirring until the ethanol concentration of the extract reached 80%. The extract was allowed to stand for 12 hours, filtered through medium-speed filter paper, and the filtrate was concentrated under reduced pressure to recover the ethanol. The solution was observed for clarity, weighed, tasted, and tested for salidroside content.

[0040] Comparative Example 2:

[0041] The Rhodiola rosea extract from step 1) of the above embodiment was heated to 50-60°C, and a 5.0% gelatin solution was added and stirred evenly. The amount of gelatin solution added was 0.25% (w / v) of the extract volume. The solution was slowly added while stirring, and allowed to stand for 12 hours. The solution was filtered with medium-speed filter paper, the clarity of the solution was observed, the weight was weighed, the taste was tested, and the salidroside content was detected.

[0042] Comparative Example 3:

[0043] Take the Rhodiola rosea extract from step 1) of the above example, slowly cool it to 0°C to 4°C, then further cool it to below -20°C. After freezing for 3 days, slowly thaw it and filter it through medium-speed filter paper. Observe the clarity of the solution, weigh it, taste it, and test the salidroside content.

[0044] Table 1 Comparison of the calcium hydroxide method of the present invention and the astringency removal method in the prior art: Experimental phenomenon Taste after filtration Salidroside retention rate Comparative Example 1 (alcohol precipitation method) A small amount of precipitation was produced, filtration was difficult, and the filtrate was turbid. It tastes astringent and hard to swallow. No testing required Comparative Example 2 (Gelatin Flocculation Method) A lot of precipitation was produced, making filtration difficult. The filtrate was clear, but the color turned light yellow. It has little astringency and has the aroma of the original medicinal material, but also has the off-flavor of gelatin. 76.48% Comparative Example 3 (freezing method) No precipitation is produced, filtration is difficult, and the filtrate is turbid. Astringent, hard to swallow No testing required Example (calcium hydroxide method) A precipitate is produced, which is easily filtered and the filtered solution becomes clear. It has little astringency, has the aroma of the original medicinal materials, and is very smooth in taste. 90.28%

[0045] Beneficial effects:

[0046] In the preparation method of the Rhodiola rosea oral liquid of the present invention, Rhodiola rosea is synergistically combined with Chinese medicinal materials such as Northern Adenophora, Lycium barbarum, and Angelica sinensis, which not only has a complementary effect on medicinal efficacy, but also significantly improves the original bitter taste of Rhodiola rosea through formula optimization. Compared with the existing technology, the retention rate of salidroside is effectively improved, and the compliance and clinical adaptability of the preparation are improved.

[0047] Test example:

[0048] 1. Animal testing

[0049] 1.1 Normal pressure hypoxia resistance test:

[0050] Mice were randomly divided into four groups: three experimental groups and one negative control group, with 12 mice in each group. The experimental groups were gavaged with three doses of the sample solution: 1.67, 3.33, and 6.67 ml / kg / BW (equivalent to 5, 10, and 20 times the recommended human dose, respectively). The blank control group was gavaged with an equal volume of distilled water (0.4 mL / 20 g / BW). The test substance was administered continuously for 30 days. One hour after the last gavage, the mice were observed for hypoxia-induced death under normal pressure and the time from decapitation to cessation of panting.

[0051] The effects of hypoxia and death time on mice at normal pressure are shown in Table 1. There were no significant differences in the hypoxia and death time on mice at normal pressure between the test substance groups and the negative control group (P>0.05). However, the test substance had a tendency to prolong the hypoxia and death time on mice at normal pressure.

[0052] Table 2 Effects of the test substances on the time to death of mice due to hypoxia under normal pressure (z±8): Dosage group (ml / kg.BW) Number of animals Time to death due to hypoxia (min) P-value 6.6 12 28.8±2.1 0.071 3.33 12 28.4+3.1 0.155 1.67 12 27.0±2.6 0.895 Blank control group 12 26.4±2.3 ------

[0053] The above results show that the high-dose oral solution of the present invention can significantly prolong the survival time of mice under normobaric hypoxia, indicating that the oral solution of the present invention has the effect of enhancing the ability of mice to tolerate normobaric hypoxia.

[0054] 1.2 Experiment on the effect on endurance of mice with spleen deficiency:

[0055] Mice were randomly divided into four groups: three experimental groups and one negative control group, with 12 mice in each group. The experimental groups were gavaged with the sample solution at three doses: 1.67, 3.33, and 6.67 ml / kg / body weight (equivalent to 5, 10, and 20 times the recommended human dose, respectively). The blank control group was gavaged with an equal volume of distilled water (0.4 mL / 20 g / body weight). Administration was continued for six days. Thirty minutes after the last dose, mice were placed in a swimming position loaded with 15% of their body weight. The water temperature was 40-42°C. The swimming time of the mice was recorded, and the results were statistically analyzed. See Table 3 for details.

[0056] Table 3 Effect of the oral solution of the present invention on endurance of mice with spleen deficiency: Dosage group (ml / kg.BW) Number of animals Reserpine (mg / Kg) Swimming time (seconds) X±SD 6.6 12 5 77.37±23.01 3.33 12 5 73.15±26.78 1.67 12 5 70.13±28.59 Blank control group 12 0 65.71±21.23 Spleen deficiency model group 12 5 51.43±15.71

[0057] The above results show that there is a significant difference between the spleen deficiency model group and the blank control group, indicating that the model is established. The oral liquid of the present invention can significantly prolong the high-temperature swimming time of the "spleen deficiency" type mice caused by reserpine, indicating that the compound Rhodiola rosea oral liquid has a significant anti-fatigue and endurance-enhancing effect.

[0058] Although the present invention has been described herein with reference to a number of illustrative embodiments thereof, it will be understood that numerous other modifications and implementations may be devised by those skilled in the art that fall within the scope and spirit of the principles disclosed herein. More specifically, within the scope of the present disclosure and claims, various variations and modifications may be made to the components and / or layout of the subject combination arrangement. In addition to variations and modifications to the components and / or layout, other uses will also be apparent to those skilled in the art.

Claims

1. A Rhodiola rosea oral liquid for removing astringency, characterized by: Its ingredients include Rhodiola rosea, Glehnia littoralis, wolfberry, Chinese angelica and honey.

2. The astringency-removing Rhodiola rosea oral liquid according to claim 1, characterized in that: The rhodiola rosea oral liquid comprises the following components: 1800-2200 parts of rhodiola rosea, 2100-2700 parts of northern adenophora, 2900-3300 parts of wolfberry, 2600-3100 parts of angelica, and 1700-2200 parts of honey.

3. The astringency-removing Rhodiola rosea oral liquid according to claim 2, characterized in that: The Rhodiola rosea oral liquid comprises the following components: 2000 parts of Rhodiola rosea, 2500 parts of Adenophora adenophora, 3000 parts of wolfberry, 3000 parts of angelica, and 2000 parts of honey.

4. A method for preparing a rhodiola rosea oral liquid for removing astringency according to any one of claims 1 to 3, characterized in that: The following steps are involved: 1) crushing the Rhodiola rosea medicinal material, extracting it with ethanol under reflux at 60-85° C. for multiple extractions, each for 1 hour, combining the extracts, filtering, and concentrating to recover the ethanol to obtain a Rhodiola rosea extract concentrate; diluting the concentrate with water to 1.2 times the weight of the Rhodiola rosea medicinal material, adding calcium hydroxide suspension to adjust the pH to 10-11, allowing the mixture to settle, filtering to obtain a filtrate, and then adjusting the pH of the filtrate to 6-7 with a 5% citric acid aqueous solution to obtain a Rhodiola rosea extract for removing astringency; 2) Crush the Radix Adenophorae, Lycium barbarum, and Angelica sinensis herbs, add 10 times the weight of the herbs to the decoction, and boil for 30 minutes at 90-100°C multiple times. Combine the extracts, filter, and concentrate to 1.5 times the weight of the original herbs. Refrigerate and centrifuge to remove impurities to obtain a clear Chinese herbal extract. 3) The Rhodiola rosea extract from step 1) and the Chinese herbal extract from step 2) are evenly mixed, refined honey prepared from a prescribed amount of honey is added, and the volume is adjusted to the target volume with purified water. After filtering, the mixture is packaged into 20 ml bottles of oral solution, sterilized by moist heat at 121°C for 30 minutes, and packaged to obtain the finished product after passing the light inspection.

5. The method for preparing the astringency-removing Rhodiola rosea oral liquid according to claim 4, wherein: The ethanol reflux extraction is performed twice in step 1).

6. The method for preparing the astringency-removing Rhodiola rosea oral liquid according to claim 4, wherein: The ethanol concentration in step 1) is 65%.

7. The method for preparing the astringency-removing Rhodiola rosea oral liquid according to claim 4, wherein: The concentration of calcium hydroxide in step 1) is 10%.

8. The method for preparing the astringency-removing Rhodiola rosea oral liquid according to claim 4, wherein: The step 1) is performed by allowing the sediment to stand for no less than 24 hours.

9. The method for preparing the astringency-removing Rhodiola rosea oral liquid according to claim 4, wherein: The step 2) is performed by allowing the sediment to stand for no less than 24 hours.

10. The method for preparing the astringency-removing Rhodiola rosea oral liquid according to claim 4, characterized in that: The purified water decoction extraction times in step 2) is 2 times.