Fermented soup for improving growth and immune function of broiler chickens and preparation method thereof

Abstract

The present application belongs to the technical field of animal health care, and particularly relates to a fermented decoction for improving growth and immune function of broilers and a preparation method thereof. The fermented decoction for improving growth and immune function of broilers is prepared from the following raw materials by fermentation according to weight parts: radix codonopsis 8-12 parts, angelica sinensis 3-7 parts, cornus officinalis 8-12 parts, cinnamomum cassia 3-7 parts and portulaca oleracea 3-7 parts. The fermented decoction significantly increases the body weight of broilers, significantly improves the thymus index and spleen index, and increases the IgG and IgM contents in serum, thereby promoting the growth of broilers and improving the immune function of broilers. The preparation method of the fermented decoction is simple, and is suitable for industrialized production and market promotion and application.

Description

Technical Field

[0001] This invention belongs to the field of animal health technology, specifically relating to a fermented soup preparation for improving the growth and immune function of broilers and its preparation method. Background Technology

[0002] Traditional Chinese medicine (TCM) fermentation technology utilizes the action of enzymes and microorganisms. Under suitable cultivation conditions, processed medicinal materials undergo fermentation, catalyzed by fungi and enzymes. During fermentation, TCM can alter its medicinal properties, enhance or generate new effects, expand its application range, and meet clinical needs. As a traditional processing method for TCM, fermentation results in TCM with unique material composition and medicinal effects.

[0003] In the practice of raising meat ducks, the application of fermented traditional Chinese medicine has not only effectively promoted the healthy growth of meat ducks, but also played an important role in improving their immune function and enhancing meat quality.

[0004] Fermentation strains are the foundation of traditional Chinese medicine (TCM) fermentation and the key to its success. Different microorganisms can metabolize and utilize different substances. Selecting suitable microorganisms as fermentation strains for different TCM varieties to maximize drug utilization requires extensive experimental research.

[0005] Therefore, how to provide a fermented product that is simple to prepare and easy to promote and apply, which can improve the growth and immune function of broilers, is a technical problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0006] The purpose of this invention is to address the shortcomings of existing technologies by providing a fermented decoction and its preparation method for improving broiler growth and immune function. This fermented decoction significantly increases broiler weight, significantly improves thymus and spleen indices, and increases serum IgG and IgM levels, thereby promoting broiler growth while simultaneously improving broiler immune function.

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] The first objective of this invention is to provide a fermented soup preparation that improves the growth and immune function of broilers.

[0009] The second objective of this invention is to provide a method for preparing the above-mentioned fermented soup for improving the growth and immune function of broilers.

[0010] The third objective of this invention is to provide the use of the above-mentioned fermented soup for improving the growth and immune function of broilers.

[0011] To achieve the first objective, the technical solution adopted by this invention is as follows:

[0012] This invention provides a fermented decoction to improve the growth and immune function of broilers. The fermented decoction is made from the following Chinese herbal raw materials in parts by weight through fermentation: 8-12 parts of Codonopsis pilosula, 3-7 parts of Angelica sinensis, 8-12 parts of Cornus officinalis, 3-7 parts of Cinnamomum cassia, and 3-7 parts of Portulaca oleracea.

[0013] Furthermore, the fermented decoction is made from the following Chinese medicinal materials in parts by weight through fermentation: 10 parts Codonopsis pilosula, 5 parts Angelica sinensis, 10 parts Cornus officinalis, 5 parts Cinnamomum cassia, and 5 parts Portulaca oleracea.

[0014] Furthermore, the fermented decoction is made from the following Chinese medicinal materials in parts by weight through fermentation: 8 parts Codonopsis pilosula, 7 parts Angelica sinensis, 8 parts Cornus officinalis, 7 parts Cinnamomum cassia, and 3 parts Portulaca oleracea.

[0015] To achieve the second objective, the technical solution adopted by this invention is as follows:

[0016] A method for preparing a fermented broth to improve the growth and immune function of broilers includes the following steps:

[0017] (1) Take Codonopsis pilosula, Angelica sinensis, Cornus officinalis, Cinnamomum cassia and Portulaca oleracea, wash them, dry them, pulverize them and pass them through an 80-mesh sieve, sterilize them at 121℃ for 30 minutes, let them cool and set them aside;

[0018] (2) After activating the compound bacterial agent by inoculating it into MRS solid medium, it was then inoculated into MRS liquid medium to obtain a colony count of 10-1. 9 CFU / ml bacterial suspension;

[0019] (3) Add the bacterial suspension from step (2) to the mixture from step (1), with the weight ratio of bacterial suspension to mixture being 0.05-0.07:10-15. Mix and ferment at 25-35℃ in a closed and static manner for 72-96 hours.

[0020] (4) After fermentation is complete, the fermentation liquid is centrifuged and filtered with activated carbon to obtain the supernatant.

[0021] Furthermore, the compound bacterial agent is composed of Trichoderma, Bacillus subtilis, Lactobacillus plantarum, and Pediococcus lactis, wherein the weight ratio of Trichoderma, Bacillus subtilis, Lactobacillus plantarum, and Pediococcus lactis is 1-3:1-3:2-4:2-4.

[0022] Furthermore, the weight ratio of Trichoderma, Bacillus subtilis, Lactobacillus plantarum, and Pediococcus lactis in the compound bacterial agent is 1:2:3:3.

[0023] Furthermore, in step (3), the weight ratio of the bacterial suspension to the mixture is 0.06:10.

[0024] Furthermore, in step (3), the mixture and bacterial suspension are fermented at 30°C for 92 hours.

[0025] To achieve the third objective, the technical solution adopted by this invention is as follows:

[0026] This invention provides the use of the fermented decoction for improving broiler growth and immune function in the preparation of drugs for preventing or improving broiler growth and immune function.

[0027] The efficacy of the traditional Chinese medicine raw materials in this invention is as follows:

[0028] Codonopsis pilosula: sweet in taste, neutral in nature, enters the lung and spleen meridians, replenishes the middle qi, quenches thirst, strengthens the spleen and benefits the lungs, nourishes blood and generates body fluids; used for spleen and lung qi deficiency, poor appetite and fatigue, cough and wheezing, qi and blood deficiency, sallow complexion, palpitations and shortness of breath, thirst due to fluid depletion, and internal heat and thirst.

[0029] Angelica sinensis: It is pungent and bitter in taste, and warm in nature. It enters the heart, liver and spleen meridians. It nourishes blood and promotes blood circulation, regulates menstruation and relieves pain, and moistens dryness and lubricates the intestines. It is used for irregular menstruation, amenorrhea, dysmenorrhea, abdominal masses, metrorrhagia, abdominal pain due to deficiency and cold, weakness and numbness, numbness of the skin, constipation due to intestinal dryness, tenesmus after dysentery, carbuncles and sores, and injuries from falls and blows.

[0030] Cornus officinalis: It tastes sour and astringent, and is slightly warm in nature. It enters the liver and kidney meridians, and nourishes the liver and kidneys, astringes essence and consolidates the body. It is used for dizziness and tinnitus, soreness and pain in the waist and knees, impotence and seminal emission, enuresis and frequent urination, metrorrhagia and leukorrhea, and profuse sweating and collapse.

[0031] Cinnamon: It has a pungent and sweet taste, is very hot in nature, and enters the kidney, spleen, and heart meridians. It tonifies fire and assists yang, dispels cold and relieves pain, and invigorates blood and regulates menstruation.

[0032] Purslane: It tastes sour and is cold in nature. It clears heat and detoxifies, cools the blood and stops dysentery, removes dampness and promotes urination. It is used for damp-heat accumulation in the lower body, yellow, sticky and foul-smelling leukorrhea, short and yellow urine, thirst and bitter taste in the mouth, red tongue with yellow coating and slippery pulse. It can also be used for damp-heat diarrhea, dysentery, etc.

[0033] The traditional Chinese medicine of this invention undergoes fermentation with probiotics, which causes certain components of the medicine to decompose and transform into new drug components. In particular, the various enzymes produced by probiotics can promote the full absorption and utilization of the effective components of the medicine by the animal body. Meanwhile, the medicine contains abundant nutrients that can promote the proliferation of probiotics. The two complement each other and better exert the efficacy of the medicine. The traditional Chinese medicine fermented with compound probiotics has both the effects of traditional Chinese medicine and the beneficial function of microorganisms.

[0034] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0035] The fermented decoction of this invention fully utilizes the effects of probiotics, traditional Chinese medicine and their fermentation products, and can significantly promote the growth of broilers and improve their immune function.

[0036] The fermented decoction of this invention significantly increases the weight of broiler chickens, significantly improves the thymus index and spleen index, and increases the content of IgG and IgM in serum. While promoting the growth of broiler chickens, it also improves their immune function. The preparation method of the fermented decoction of this invention is simple and suitable for industrial production and market application. Attached Figure Description

[0037] Figure 1 Comparison of weights in each group

[0038] Compared with the control group, P < 0.05 P < 0.01; compared with Example 1 group, # P < 0.05 ## P < 0.01.

[0039] Figure 2 Comparison of average daily weight gain among groups

[0040] Compared with the control group, P < 0.05 P < 0.01; compared with Example 1 group, # P < 0.05 ## P < 0.01.

[0041] Figure 3 Comparison of spleen indices among groups

[0042] Compared with the control group, P < 0.05 P < 0.01; compared with Example 1 group, # P < 0.05 ## P < 0.01.

[0043] Figure 4 Comparison of bursa of Fabricius indices among different groups.

[0044] Figure 5 Comparison of thymus index among groups

[0045] Compared with the control group, P < 0.05 P < 0.01; compared with Example 1 group, # P < 0.05.

[0046] Figure 6 Comparison of IgG levels in each group

[0047] Compared with the control group, P < 0.05 P < 0.01; compared with Example 1 group, # P < 0.05 ## P < 0.01.

[0048] Figure 7 Comparison of IgM content in each group

[0049] Compared with the control group, P < 0.05 P < 0.01; compared with Example 1 group, # P < 0.05.

[0050] Figure 8 Comparison of IgA content in each group. Detailed Implementation

[0051] The embodiments of the present invention will be described in detail below with reference to examples. However, those skilled in the art will understand that the following examples are only for illustrating the present invention and should not be regarded as limiting the scope of the present invention.

[0052] Example 1

[0053] A fermented soup for preventing or improving the growth and immune function of broilers is made from the following raw materials through fermentation: 10g of Codonopsis pilosula, 5g of Angelica sinensis, 10g of Cornus officinalis, 5g of Cinnamomum cassia and 5g of Portulaca oleracea.

[0054] The preparation method is as follows:

[0055] (1) Take Codonopsis pilosula, Angelica sinensis, Cornus officinalis, Cinnamomum cassia and Portulaca oleracea, wash them, dry them, pulverize them and pass them through an 80-mesh sieve, sterilize them at 121℃ for 30 minutes, let them cool and set them aside;

[0056] (2) After activating the compound bacterial agent by inoculating it into MRS solid medium, it was then inoculated into MRS liquid medium to obtain a colony count of 10-1. 9 CFU / ml bacterial suspension;

[0057] (3) Add the bacterial suspension from step (2) to the mixture from step (1), with a weight ratio of bacterial suspension to mixture of 0.06:10. Mix, and ferment in a closed, static environment at 30°C for 92 hours.

[0058] (4) After fermentation is complete, the fermentation liquid is centrifuged and filtered with activated carbon to obtain the supernatant.

[0059] The weight ratio of Trichoderma, Bacillus subtilis, Lactobacillus plantarum, and Pediococcus lactis in the compound bacterial agent is 1:2:3:3.

[0060] Example 2

[0061] A fermented soup for preventing or improving the growth and immune function of broilers is made from the following raw materials through fermentation: 8g of Codonopsis pilosula, 7g of Angelica sinensis, 8g of Cornus officinalis, 7g of Cinnamomum cassia and 3g of Portulaca oleracea.

[0062] The preparation method is as follows:

[0063] (1) Take Codonopsis pilosula, Angelica sinensis, Cornus officinalis, Cinnamomum cassia and Portulaca oleracea, wash them, dry them, pulverize them and pass them through an 80-mesh sieve, sterilize them at 121℃ for 30 minutes, let them cool and set them aside;

[0064] (2) After activating the compound bacterial agent by inoculating it into MRS solid medium, it was then inoculated into MRS liquid medium to obtain a colony count of 10-1. 9 CFU / ml bacterial suspension;

[0065] (3) Add the bacterial suspension from step (2) to the mixture from step (1), with a weight ratio of bacterial suspension to mixture of 0.07:10. Mix, and ferment in a closed, static environment at 25°C for 96 hours.

[0066] (4) After fermentation is complete, the fermentation liquid is centrifuged and filtered with activated carbon to obtain the supernatant.

[0067] The weight ratio of Trichoderma, Bacillus subtilis, Lactobacillus plantarum, and Pediococcus lactis in the compound bacterial agent is 1:1:4:4.

[0068] Example 3

[0069] A fermented soup for preventing or improving the growth and immune function of broilers is made from the following raw materials through fermentation: Codonopsis pilosula 12g, Angelica sinensis 3g, Cornus officinalis 12g, Cinnamomum cassia 3g and Portulaca oleracea 7g.

[0070] The preparation method is as follows:

[0071] (1) Take Codonopsis pilosula, Angelica sinensis, Cornus officinalis, Cinnamomum cassia and Portulaca oleracea, wash them, dry them, pulverize them and pass them through an 80-mesh sieve, sterilize them at 121℃ for 30 minutes, let them cool and set them aside;

[0072] (2) After activating the compound bacterial agent by inoculating it into MRS solid medium, it was then inoculated into MRS liquid medium to obtain a colony count of 10-1. 9 CFU / ml bacterial suspension;

[0073] (3) Add the bacterial suspension from step (2) to the mixture from step (1), with a weight ratio of bacterial suspension to mixture of 0.05:15. Mix, and ferment in a closed and static manner at 35°C for 72 hours.

[0074] (4) After fermentation is complete, the fermentation liquid is centrifuged and filtered with activated carbon to obtain the supernatant.

[0075] The weight ratio of Trichoderma, Bacillus subtilis, Lactobacillus plantarum, and Pediococcus lactis in the compound bacterial agent is 3:1:2:4.

[0076] Example 4

[0077] A fermented soup for preventing or improving the growth and immune function of broilers is made from the following raw materials through fermentation: 10g of Codonopsis pilosula, 7g of Angelica sinensis, 8g of Cornus officinalis, 5g of Cinnamomum cassia and 7g of Portulaca oleracea.

[0078] The preparation method is as follows:

[0079] (1) Take Codonopsis pilosula, Angelica sinensis, Cornus officinalis, Cinnamomum cassia and Portulaca oleracea, wash them, dry them, pulverize them and pass them through an 80-mesh sieve, sterilize them at 121℃ for 30 minutes, let them cool and set them aside;

[0080] (2) After activating the compound bacterial agent by inoculating it into MRS solid medium, it was then inoculated into MRS liquid medium to obtain a colony count of 10-1. 9 CFU / ml bacterial suspension;

[0081] (3) Add the bacterial suspension from step (2) to the mixture from step (1), with a weight ratio of bacterial suspension to mixture of 0.07:15. Mix, and ferment in a closed and static manner at 30°C for 72 hours.

[0082] (4) After fermentation is complete, the fermentation liquid is centrifuged and filtered with activated carbon to obtain the supernatant.

[0083] The weight ratio of Trichoderma, Bacillus subtilis, Lactobacillus plantarum, and Pediococcus lactis in the compound bacterial agent is 1:3:2:2.

[0084] Example 5

[0085] A fermented soup for preventing or improving the growth and immune function of broilers is made from the following raw materials through fermentation: 12g of Codonopsis pilosula, 5g of Angelica sinensis, 10g of Cornus officinalis, 7g of Cinnamomum cassia and 3g of Portulaca oleracea.

[0086] The preparation method is as follows:

[0087] (1) Take Codonopsis pilosula, Angelica sinensis, Cornus officinalis, Cinnamomum cassia and Portulaca oleracea, wash them, dry them, pulverize them and pass them through an 80-mesh sieve, sterilize them at 121℃ for 30 minutes, let them cool and set them aside;

[0088] (2) After activating the compound bacterial agent by inoculating it into MRS solid medium, it was then inoculated into MRS liquid medium to obtain a colony count of 10-1. 9 CFU / ml bacterial suspension;

[0089] (3) Add the bacterial suspension from step (2) to the mixture from step (1), with a weight ratio of bacterial suspension to mixture of 0.05:10. Mix, and ferment in a closed and static manner at 35°C for 72 hours.

[0090] (4) After fermentation is complete, the fermentation liquid is centrifuged and filtered with activated carbon to obtain the supernatant.

[0091] The weight ratio of Trichoderma, Bacillus subtilis, Lactobacillus plantarum, and Pediococcus lactis in the compound bacterial agent is 2:3:3:2.

[0092] Comparative Example 1

[0093] A fermented decoction for preventing or improving the growth and immune function of broilers is made from the following raw materials through fermentation: 10g of Angelica sinensis, 15g of Cornus officinalis, 5g of Cinnamomum cassia and 5g of Portulaca oleracea.

[0094] Compared to Example 1, the difference is that it does not contain Codonopsis pilosula, and the amount of Codonopsis pilosula is increased to the amount of Angelica sinensis and Cornus officinalis. The preparation method is the same as in Example 1.

[0095] Comparative Example 2

[0096] A fermented soup for preventing or improving the growth and immune function of broilers is made from the following raw materials through fermentation: 12g of Codonopsis pilosula, 13g of Cornus officinalis, 5g of Cinnamomum cassia and 5g of Portulaca oleracea.

[0097] Compared to Example 1, the difference is that it does not contain Angelica sinensis, and the amount of Angelica sinensis is increased in the amounts of Codonopsis pilosula and Cornus officinalis. The preparation method is the same as in Example 1.

[0098] Comparative Example 3

[0099] A fermented soup for preventing or improving the growth and immune function of broilers is made from the following raw materials through fermentation: 10g of Codonopsis pilosula, 10g of Angelica sinensis, 10g of cinnamon and 5g of Portulaca oleracea.

[0100] Compared to Example 1, the difference is that it does not contain Cornus officinalis, and the amount of Cornus officinalis is increased in relation to the amounts of Angelica sinensis and cinnamon. The preparation method is the same as in Example 1.

[0101] Comparative Example 4

[0102] A fermented soup for preventing or improving the growth and immune function of broilers is made from the following raw materials through fermentation: 10g of Codonopsis pilosula, 5g of Angelica sinensis, 10g of Cornus officinalis, 5g of Cinnamomum cassia and 5g of Portulaca oleracea.

[0103] Compared with Example 1, the difference lies in the fermentation agent. The compound agent is composed of Lactobacillus plantarum and Pediococcus lactis in a weight ratio of 3:3, and the weight ratio of the bacterial suspension to the mixture is the same.

[0104] Comparative Example 5

[0105] A fermented soup for preventing or improving the growth and immune function of broilers is made from the following raw materials through fermentation: 10g of Codonopsis pilosula, 5g of Angelica sinensis, 10g of Cornus officinalis, 5g of Cinnamomum cassia and 5g of Portulaca oleracea.

[0106] Compared with Example 1, the difference lies in the fermentation agent. The compound inoculum consists of Bacillus subtilis, Lactobacillus plantarum and Pediococcus lactis in a weight ratio of 3:3:3, and the weight ratio of the bacterial suspension to the mixture is the same.

[0107] Comparative Example 6

[0108] A fermented soup for preventing or improving the growth and immune function of broilers is made from the following raw materials through fermentation: 10g of Codonopsis pilosula, 5g of Angelica sinensis, 10g of Cornus officinalis, 5g of Cinnamomum cassia and 5g of Portulaca oleracea.

[0109] Compared with Example 1, the difference lies in the fermentation agent. The compound inoculum consists of Trichoderma, Lactobacillus plantarum and Pediococcus lactis in a weight ratio of 3:3:3, and the weight ratio of the bacterial suspension to the mixture is the same.

[0110] Experimental Example: Effects of the Fermented Broth of the Present Invention on the Growth Performance and Immune Function of Broilers

[0111] 1. Experimental Methods

[0112] Nine hundred one-day-old broiler chickens were randomly divided into nine groups of 100 each. The control group, Example 1 group, Example 2 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, Comparative Example 5 group, and Comparative Example 6 group received 2.5% fermented decoction in their drinking water. The control group received no medication. The experiment lasted 42 days. The basal diet for the broiler chickens was formulated according to the broiler nutrient levels in the NRC (2012) and divided into two phases: 1-3 weeks of age and 4-6 weeks of age. The composition and nutrient levels of the basal diet are shown in Table 1.

[0113] Table 1. Composition and nutrient levels of basal diets (dry matter basis)

[0114]

[0115] Before the experiment, the chicken house was thoroughly rinsed and fumigated for disinfection. The experimental chickens were housed in three cages (top, middle, and bottom). 24 hours before chicks were introduced, the chicken house temperature was raised to 32-35℃, and then decreased by 2-3℃ weekly until it was maintained at 21-24℃. Continuous lighting and natural ventilation were used. Throughout the experiment, the chickens had free access to feed and water, and were vaccinated according to standardized chicken house management procedures.

[0116] 2. Sample collection and indicator detection

[0117] 2.1 Growth performance

[0118] On day 42 of the experiment, the fasting weight of broiler chickens was measured after 12 hours of fasting. The average daily weight gain was calculated as follows: Average daily weight gain = Total weight gain / (Number of trials × Number of trial days).

[0119] 2.2 Immune indicators

[0120] On day 42 of the experiment, 10 mL of blood was collected from the subwing vein of 5 broiler chickens in each group. After standing for 4 hours, the blood was centrifuged at 4000 rpm for 6 minutes to obtain the supernatant serum. The broiler chickens from which the blood was collected were slaughtered, and their spleen, bursa of Fabricius, and thymus were weighed. Serum immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) were measured. Organ index = organ weight / pre-slaughter live weight × 100%.

[0121] 3. Statistical Methods

[0122] Data processing was performed using Graphpad Prism 9.0 software. Quantitative data were expressed as mean ± standard deviation. This indicates that one-way ANOVA was used for comparisons among multiple groups, and pairwise comparisons between groups were performed using SNK-q, with P < 0.05 considered statistically significant.

[0123] 4. Experimental Results

[0124] 4.1 Effects on broiler growth performance

[0125] Compared with the control group, Example 1 and Example 2 groups significantly increased the body weight of broiler chickens at 42 days of age. Example 1 group showed the best effect in increasing body weight, significantly better than Comparative Examples 1, 2, 3, 4, 5, and 6. Results are shown below. Figure 1 .

[0126] Compared with the control group, both Example 1 and Example 2 groups significantly increased the average daily weight gain of broiler chickens. Example 1 group showed the best effect in increasing average daily weight gain, significantly better than Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, Comparative Example 5, and Comparative Example 6. Results are shown below. Figure 2 .

[0127] 4.2 Effects on immune function

[0128] Compared with the control group, the Example 1 and Example 2 groups significantly improved the thymus index and spleen index of broiler chickens. The Example 1 group showed the best effect in improving the thymus index and spleen index, significantly better than the Comparative Groups 1, 2, 3, 4, 5, and 6. Results are shown below. Figure 3 , 4 5.

[0129] Compared with the control group, the Example 1 and Example 2 groups significantly increased the levels of IgG and IgM in broiler serum. The Example 1 group showed the best effect in increasing the levels of IgG and IgM in broiler serum, significantly superior to Comparative Examples 1, 2, 3, 4, 5, and 6. Results are shown below. Figure 6, 7 8.

[0130] The fermented decoction of this invention significantly increases the weight of broiler chickens, significantly improves the thymus index and spleen index, and increases the content of IgG and IgM in serum. While promoting the growth of broiler chickens, it also improves their immune function.

[0131] The present invention has been described in detail above with general descriptions and specific embodiments. However, modifications or improvements can be made to the present invention, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.

Claims

1. A fermented soup preparation for improving the growth and immune function of broilers, characterized in that, The fermented decoction is made from the following Chinese medicinal materials in parts by weight through fermentation: Codonopsis pilosula 8-12 parts, Angelica sinensis 3-7 parts, Cornus officinalis 8-12 parts, Cinnamomum cassia 3-7 parts, and Portulaca oleracea 3-7 parts; the fermentation agent is a compound microbial agent, which is composed of Trichoderma, Bacillus subtilis, Lactobacillus plantarum, and Pediococcus lactis, wherein the weight ratio of Trichoderma, Bacillus subtilis, Lactobacillus plantarum, and Pediococcus lactis is 1-3:1-3:2-4:2-4.

2. The fermented decoction for improving broiler growth and immune function according to claim 1, characterized in that, The fermented decoction is made from the following Chinese medicinal materials in parts by weight through fermentation: 10 parts Codonopsis pilosula, 5 parts Angelica sinensis, 10 parts Cornus officinalis, 5 parts Cinnamomum cassia and 5 parts Portulaca oleracea.

3. The fermented soup preparation for improving broiler growth and immune function according to claim 1, characterized in that, The fermented decoction is made from the following Chinese medicinal materials in parts by weight through fermentation: 8 parts Codonopsis pilosula, 7 parts Angelica sinensis, 8 parts Cornus officinalis, 7 parts Cinnamomum cassia, and 3 parts Portulaca oleracea.

4. The fermented decoction for improving broiler growth and immune function according to any one of claims 1-3, characterized in that, The preparation method of the fermented soup that improves the growth and immune function of broilers is as follows: (1) Take Codonopsis pilosula, Angelica sinensis, Cornus officinalis, Cinnamomum cassia and Portulaca oleracea, wash them, dry them, crush them and pass them through an 80-mesh sieve, sterilize them at 121℃ for 30 minutes, let them cool and set them aside; (2) After activating the compound bacterial agent by inoculating it into MRS solid medium, it was then inoculated into MRS liquid medium to obtain a colony count of 10-1. 9 CFU / ml bacterial suspension; (3) Add the bacterial suspension from step (2) to the mixture from step (1), with the weight ratio of bacterial suspension to mixture being 0.05-0.07:10-15. Mix and ferment at 25-35℃ in a closed and static manner for 72-96 hours. (4) After fermentation is complete, the fermentation liquid is centrifuged and filtered with activated carbon to obtain the supernatant.

5. The fermented broth for improving growth and immune function of broiler chickens according to claim 4, wherein the fermented broth is prepared by fermenting the mixture of the raw materials in the presence of the microorganism. The weight ratio of Trichoderma, Bacillus subtilis, Lactobacillus plantarum, and Pediococcus lactis in the compound bacterial agent is 1:2:3:

3.

6. The fermented soup preparation for improving broiler growth and immune function according to claim 4, characterized in that, In step (3), the weight ratio of bacterial suspension to mixture is 0.06:

10.

7. The fermented broth for improving growth and immune function of broiler chickens according to claim 4, wherein the fermented broth is prepared by fermenting the mixture of the raw materials in the presence of the microorganism. In step (3), the mixture and bacterial suspension are fermented at 30°C for 92 hours.

8. The use of the fermented decoction for improving broiler growth and immune function according to any one of claims 1-3 in the preparation of drugs for improving broiler growth and immune function.

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