Method of artificial planting northerly Chinese caterpillar fungus for increasing content of physiologically active substance

A physiologically active substance, the technology of Cordyceps militaris, which is applied in the direction of fungi, can solve the problem of high content of physiologically active substances, and achieve the effects of low cost, easy process and simple method

Inactive Publication Date: 2003-10-01
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] With the widespread utilization of Cordyceps sinensis, the research on artificial cultivation and fermentation to produce mycelium to replace natural Cordyceps sinensis is becoming more and more active. Culture medium, artificial cultivation of Cordycep

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Operate as follows:

[0024] (1) Strain separation: take fresh wild Cordyceps militaris, wash, and use 0.1% concentration of HgCL 2 , as a surface disinfectant, after 5 minutes, rinse with sterile water 5 times, cut it into thin slices under sterile conditions, and set aside;

[0025] (2) Incline strain culture: 20 grams of glucose, 10 grams of octopus peptone, 20 grams of casein, 0.3 grams of potassium dihydrogen phosphate, 0.5 grams of magnesium sulfate, V B1 , V B2 Trace amount, 1.8 grams of agar, 200 grams of potatoes, steps: peel and chop the potatoes, add 5 times the water, boil the juice, filter, remove the slag, add other ingredients to make 1000 ml; heat to melt, adjust the pH to 6.5, pack Put it into a 18×180mm test tube for sterilization, sterilize at 115°C for 25 minutes, then make the medium in the test tube into a slant, put the thinly sliced ​​fresh wild Cordyceps militaris on the potato slant medium, and cultivate it at 22°C for 7-10 Days, grow white ...

Embodiment 2

[0032] Operate as follows:

[0033] (1) Bacteria separation: get fresh wild Cordyceps militaris, wash, adopt the ethanol of 70% concentration, as surface disinfectant, after 5 minutes, wash 7 times with sterile water, cut it into thin slices under aseptic condition, wait for use;

[0034] (2) Incline strain culture: 20 grams of glucose, 10 grams of octopus peptone, 17 grams of casein, 0.3 grams of potassium dihydrogen phosphate, 0.5 grams of magnesium sulfate, V B1 , V B2 Trace amount, agar 1.8%, potato 20%, steps: peel and chop the potato, add 7 times of water, boil the juice, filter, remove the slag and add other ingredients to make 1000ml; heat to melt, adjust the pH to 7, pack Put it into a 18×180mm test tube, sterilize it at 115°C for 25 minutes, then make the culture medium in the test tube into a slant, place the thinly sliced ​​fresh wild Cordyceps militaris on the potato slant, and cultivate it at 25°C for 7-10 days, and grow white Bacterial colony, for strain cult...

Embodiment 3

[0041] Operate as follows:

[0042] (1) Strain separation: get fresh wild Cordyceps militaris, wash, and use 30% concentration of H 2 o 2 , as a surface disinfectant, after 5 minutes, rinse with sterile water 5 times, cut it into thin slices under sterile conditions, and set aside;

[0043] (2) Incline strain culture: 20 grams of glucose, 10 grams of octopus peptone, 15 grams of casein, 0.3 grams of potassium dihydrogen phosphate, 0.5 grams of magnesium sulfate, V B1 , V B2 Trace amount, agar 1.8%, potato 20%, steps: peel and chop the potato, add 5 times of water, boil the juice, filter, remove residue and add other ingredients to make 1000ml; heat to dissolve, adjust the pH to 6, pack Put it into a 18×180mm test tube, sterilize it at 115°C for 25 minutes, then make the culture medium in the test tube into a slant, place the thinly sliced ​​fresh wild Cordyceps militaris on the potato slant, and cultivate it at 20°C for 7-10 days, and grow white Bacterial colony, for strai...

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Abstract

A method for artificially culturing north cordyceps to increase the contents of its physiological active components (cordyceptic acid, cordycepin and cordyceptic polyose) includes such steps as washing fresh wild north cordyceps, section, regulating pH of culture medium to 6-7, sterilizing, shaping slant, slant culturing at 20-25 deg.C for 7-10 days, regulating pH of liquid culture medium to 6-7,sterilizing, inoculating, culturing, preparing solid culture medium from rice and sorghum grains, inoculating, and culturing at 25-29 deg.C for 40-60 days.

Description

technical field [0001] The invention relates to Cordyceps militaris, in particular to a method for artificially cultivating Cordyceps militaris to increase the content of physiologically active substances. Background technique [0002] Cordyceps militaris and Cordyceps sinensis (wild in Tibet, Xinjiang, Qinghai, Sichuan and other mountains) belong to two different species of the genus Cordyceps. Cordyceps militaris is the model species of the genus Cordyceps. The physiologically active substances are basically the same, and the content of cordycepin It is many times that of Cordyceps sinensis. [0003] People in our country have used Cordyceps sinensis to treat diseases and keep healthy and accumulated a lot of valuable experience. Any old people who are frail, weak after illness, impotence due to stomach deficiency, nocturnal emission, premature ejaculation, frail and sick, etc., can get good results no matter it is used alone or in combination. With the development of sci...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 李维光梁文举
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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