A method for extracting and separating fat-soluble components of three-spotted hippocampus

A three-spotted hippocampus, fat-soluble technology, applied in biochemical equipment and methods, microorganisms, tissue culture, etc., can solve the problems of high cost, unclear mechanism, interference with nutrient absorption, etc., and achieve the effect of no toxic side effects

Active Publication Date: 2018-12-28
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The methods for removing cholesterol from animal tissues currently include physical methods such as organic solvent extraction, supercritical carbon dioxide extraction, β-cyclodextrin embedding, and biological methods such as decomposition of lactobacilli, which are costly or have unclear mechanisms, and other A series of unfavorable factors such as metabolites interfere with the absorption of other nutrients and its use is limited

Method used

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  • A method for extracting and separating fat-soluble components of three-spotted hippocampus
  • A method for extracting and separating fat-soluble components of three-spotted hippocampus
  • A method for extracting and separating fat-soluble components of three-spotted hippocampus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Step 1, extract fat-soluble components:

[0048] 1.1 Rinse the dried body of three-spotted hippocampus with tap water to remove sludge and sand, and then place it in a constant temperature drying oven at 60°C for 24 hours. After being pulverized by a Chinese herbal medicine grinder, pass through a 20-40 mesh sieve to obtain the three-spot hippocampus powder. The three-spot hippocampus powder is mixed with an ethanol solution with a mass fraction of 70%-95% at a material-to-liquid ratio of 1:8g / ml. Reflux at ℃ for 1 h to obtain crude ethanol extract.

[0049] 1.2 The ethanol crude extract of hippocampus three-spotted was suction-filtered in a Buchner funnel, and the filter residue was suction-filtered three times. The combined filtrates were evaporated under reduced pressure to remove the solvent to obtain extract-like ethanol crude extract.

[0050] 1.3 Mix the extract-like ethanol crude extract with deionized water at a ratio of 1:8g / ml, then add petroleum ether equa...

Embodiment 2

[0057] Step 1, extract fat-soluble components:

[0058] 1.1 Rinse the dried body of three-spotted hippocampus with tap water to remove sludge and sand, and then place it in a constant temperature drying oven at 50°C for 36 hours. After being pulverized by a Chinese herbal medicine grinder, pass through a 20-40 mesh sieve to obtain the three-spot hippocampus powder. The three-spot hippocampus powder is mixed with an ethanol solution with a mass fraction of 70%-95% at a material-to-liquid ratio of 1:12g / ml. Reflux at ℃ for 2h to obtain crude ethanol extract.

[0059] 1.2 The ethanol crude extract of hippocampus three-spotted was suction-filtered in a Buchner funnel, and the filter residue was suction-filtered three times. The combined filtrates were evaporated under reduced pressure to remove the solvent to obtain extract-like ethanol crude extract.

[0060] 1.3 Mix the extract-like ethanol crude extract with deionized water at a ratio of 1:12g / ml, then add petroleum ether equ...

Embodiment 3

[0067] Step 1, extract fat-soluble components:

[0068] 1.1 Rinse the dried three-spotted hippocampus with tap water to remove sludge and sand, and then place it in a constant temperature drying oven at 55°C for 30 hours. After being pulverized by a Chinese herbal medicine pulverizer, pass through a 20-40 mesh sieve to obtain the three-spot hippocampus powder. The three-spot hippocampus powder is mixed with an ethanol solution with a mass fraction of 70%-95% at a material-to-liquid ratio of 1:10g / ml. Reflux at ℃ for 1 h to obtain crude ethanol extract.

[0069] 1.2 The ethanol crude extract of hippocampus three-spotted was suction-filtered in a Buchner funnel, and the filter residue was suction-filtered three times. The combined filtrates were evaporated under reduced pressure to remove the solvent to obtain extract-like ethanol crude extract.

[0070] 1.3 Mix the extract-like ethanol crude extract with deionized water at a ratio of 1:10g / ml, then add petroleum ether equal t...

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Abstract

The invention discloses a method of extracting and isolating three-spot seahorse liposoluble components. The method includes: extracting liposoluble components, measuring maximum wavelength of ultraviolet absorption peaks of the components, with the maximum wavelength of ultraviolet absorption peaks as detection wavelength, subjecting the components to medium-pressure preparation and isolating and eluting by purified chromatographic silica-gel column chromatography so as to obtain three-spot seahorse liposoluble isolate with cholesterol removed. The liposoluble components are subjected to medium-pressure preparation and purified chromatographic silica-gel column chromatography, differently polar solvents are used in step / linear gradient elution, and isolate components different in polarity are obtained; the component Fr IV of high polarity has a high content of physiological activators, no cholesterol is detected in the component; meanwhile, the component Fr IV is capable of inhibiting lipopolysaccharide from stimulating mouse macrophage RAW 264.7 inflammatory factor, nitric oxide, has no toxic or side effects on cells and has no significant influence on cellular survivability.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and relates to a method for extracting and separating fat-soluble components of three-spotted hippocampus. Background technique [0002] Inflammation is the body's defense response to infection, pathogen invasion, or cell damage. Its basic biological process is the most common signal of disease. Inflammation is alleviated in most cases by the accumulation of endogenous anti-inflammatory mediators and intracellular negative regulators. However, continued accumulation and activation, hallmarks of chronic inflammation, will lead to dysfunction of these negative regulatory mechanisms. Therefore, strengthening and accelerating the limitation and resolution of inflammation is the key to the development of anti-inflammatory drugs. [0003] In recent years, the extraction of anti-inflammatory active substances from natural products has attracted more and more attention because of its saf...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/02
Inventor 申铉日陈莉萍
Owner HAINAN UNIVERSITY
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