A method for extracting and separating fat-soluble components of three-spotted hippocampus

A three-spotted hippocampus, fat-soluble technology, applied in biochemical equipment and methods, microorganisms, tissue culture, etc., can solve the problems of high cost, unclear mechanism, interference with nutrient absorption, etc., and achieve the effect of no toxic side effects
CN105030837BActive Publication Date: 2018-12-28HAINAN UNIVERSITY
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
HAINAN UNIVERSITY
Publication Date
2018-12-28

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Abstract

The invention discloses a method of extracting and isolating three-spot seahorse liposoluble components. The method includes: extracting liposoluble components, measuring maximum wavelength of ultraviolet absorption peaks of the components, with the maximum wavelength of ultraviolet absorption peaks as detection wavelength, subjecting the components to medium-pressure preparation and isolating and eluting by purified chromatographic silica-gel column chromatography so as to obtain three-spot seahorse liposoluble isolate with cholesterol removed. The liposoluble components are subjected to medium-pressure preparation and purified chromatographic silica-gel column chromatography, differently polar solvents are used in step / linear gradient elution, and isolate components different in polarity are obtained; the component Fr IV of high polarity has a high content of physiological activators, no cholesterol is detected in the component; meanwhile, the component Fr IV is capable of inhibiting lipopolysaccharide from stimulating mouse macrophage RAW 264.7 inflammatory factor, nitric oxide, has no toxic or side effects on cells and has no significant influence on cellular survivability.
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Description

technical field

[0001] The invention belongs to the technical field of biological extraction, and relates to a method for extracting and separating fat-soluble components of three-spotted hippocampus. Background technique

[0002] Inflammation is the body's defense response to infection, pathogen invasion, or cell damage. Its basic biological process is the most common signal of disease. Inflammation is alleviated in most cases by the accumulation of endogenous anti-inflammatory mediators and intracellular negative regulators. However, continued accumulation and activation, hallmarks of chronic inflammation, will lead to dysfunction of these negative regulatory mechanisms. Therefore, strengthening and accelerating the limitation and resolution of inflammation is the key to the development of anti-inflammatory drugs.

[0003] In recent years, the extraction of anti-inflammatory active substances from natural products has attracted more and more attention because of its saf...

Claims

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