Industrial method for culturing north winter worm summer herb with high-content of cordycepin
A technology of Cordyceps militaris and a cultivation method, which is applied in the field of industrialized cultivation of Cordyceps militaris with high content of cordycepin, can solve problems such as non-industrial production, and achieve the effect of simple method.
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Embodiment 1
[0052] (Improved PDA medium):
[0053] Potatoes 190g
[0054] Sucrose 21g
[0055] Agar 20g
[0056] Add water to make up 1000ml
[0057]
[0058] The parent species of Cordyceps militaris isolated from wild Cordyceps is inserted into a modified PDA (potato dextrose agar medium) medium, and cultured at 20°C±1°C in the dark for 10-12 days to obtain the original seed solution.
[0059] (Original medium):
[0060] Potatoes 200g
[0061] Sucrose 10g
[0062] Peptone 1.0g
[0063] Carrot 10g
[0064] Onion 10g
[0065] Mushroom 10g
[0066] Egg 5g
[0067] Alum 0.05g
[0068] Sodium bicarbonate 1.0g
[0069] Add water to make up 1000ml
[0070]
[0071] Add 24g of rice and 32ml of water into each culture bottle, cover it after mixing, and sterilize; add 2.5ml of the original seed solution to each culture bottle for inoculation. Transfer the seeded Cordyceps militaris culture bottle to the...
Embodiment 2
[0073] (Improved PDA medium):
[0074] Potatoes 200g
[0075] Sucrose 20g
[0076] Agar 20g
[0077] Add water to make up 1000ml
[0078]
[0079] The parent species of Cordyceps militaris isolated from wild Cordyceps is inserted into a modified PDA (potato dextrose agar medium) medium, and cultured at 20°C±1°C in the dark for 10-12 days to obtain the original seed solution.
[0080] (Original medium):
[0081] Potatoes 204g
[0082] Sucrose 9g
[0083] Peptone 1.0g
[0084] Carrot 9g
[0085] Onion 9g
[0086] Mushroom 9g
[0087] Egg 5g
[0088] Alum 0.05g
[0089] Sodium bicarbonate 1.0g
[0090] Add water to make up 1000ml
[0091]
[0092] Add 24g of rice and 32ml of water into each culture bottle, cover it after mixing, and sterilize; add 2.5ml of the original seed solution to each culture bottle for inoculation. Transfer the seeded Cordyceps militaris culture bottle to the cultu...
Embodiment 3
[0094] (Improved PDA medium):
[0095] Potatoes 190g
[0096] Sucrose 21g
[0097] Agar 20g
[0098] Add water to make up 1000ml
[0099]
[0100] The parent species of Cordyceps militaris isolated from wild Cordyceps is inserted into a modified PDA (potato dextrose agar medium) medium, and cultured at 20°C±1°C in the dark for 10-12 days to obtain the original seed solution.
[0101] (Original medium):
[0102] Potatoes 196g
[0103] Sucrose 11g
[0104] Peptone 1.0g
[0105] Carrot 11g
[0106] Onion 11g
[0107] Mushroom 11g
[0108] Egg 5g
[0109] Alum 0.05g
[0110] Sodium bicarbonate 1.0g
[0111] Add water to make up 1000ml
[0112]
[0113] Add 24g of rice and 32ml of water into each culture bottle, cover it after mixing, and sterilize; add 2.5ml of the original seed solution to each culture bottle for inoculation. Transfer the seeded Cordyceps militaris culture bottle ...
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