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Industrial method for culturing north winter worm summer herb with high-content of cordycepin

A technology of Cordyceps militaris and a cultivation method, which is applied in the field of industrialized cultivation of Cordyceps militaris with high content of cordycepin, can solve problems such as non-industrial production, and achieve the effect of simple method.

Inactive Publication Date: 2008-05-14
张刚 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding Cordyceps militaris with high content of cordycepin, there is no industrial production for various reasons

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] (Improved PDA medium):

[0053] Potatoes 190g

[0054] Sucrose 21g

[0055] Agar 20g

[0056] Add water to make up 1000ml

[0057]

[0058] The parent species of Cordyceps militaris isolated from wild Cordyceps is inserted into a modified PDA (potato dextrose agar medium) medium, and cultured at 20°C±1°C in the dark for 10-12 days to obtain the original seed solution.

[0059] (Original medium):

[0060] Potatoes 200g

[0061] Sucrose 10g

[0062] Peptone 1.0g

[0063] Carrot 10g

[0064] Onion 10g

[0065] Mushroom 10g

[0066] Egg 5g

[0067] Alum 0.05g

[0068] Sodium bicarbonate 1.0g

[0069] Add water to make up 1000ml

[0070]

[0071] Add 24g of rice and 32ml of water into each culture bottle, cover it after mixing, and sterilize; add 2.5ml of the original seed solution to each culture bottle for inoculation. Transfer the seeded Cordyceps militaris culture bottle to the...

Embodiment 2

[0073] (Improved PDA medium):

[0074] Potatoes 200g

[0075] Sucrose 20g

[0076] Agar 20g

[0077] Add water to make up 1000ml

[0078]

[0079] The parent species of Cordyceps militaris isolated from wild Cordyceps is inserted into a modified PDA (potato dextrose agar medium) medium, and cultured at 20°C±1°C in the dark for 10-12 days to obtain the original seed solution.

[0080] (Original medium):

[0081] Potatoes 204g

[0082] Sucrose 9g

[0083] Peptone 1.0g

[0084] Carrot 9g

[0085] Onion 9g

[0086] Mushroom 9g

[0087] Egg 5g

[0088] Alum 0.05g

[0089] Sodium bicarbonate 1.0g

[0090] Add water to make up 1000ml

[0091]

[0092] Add 24g of rice and 32ml of water into each culture bottle, cover it after mixing, and sterilize; add 2.5ml of the original seed solution to each culture bottle for inoculation. Transfer the seeded Cordyceps militaris culture bottle to the cultu...

Embodiment 3

[0094] (Improved PDA medium):

[0095] Potatoes 190g

[0096] Sucrose 21g

[0097] Agar 20g

[0098] Add water to make up 1000ml

[0099]

[0100] The parent species of Cordyceps militaris isolated from wild Cordyceps is inserted into a modified PDA (potato dextrose agar medium) medium, and cultured at 20°C±1°C in the dark for 10-12 days to obtain the original seed solution.

[0101] (Original medium):

[0102] Potatoes 196g

[0103] Sucrose 11g

[0104] Peptone 1.0g

[0105] Carrot 11g

[0106] Onion 11g

[0107] Mushroom 11g

[0108] Egg 5g

[0109] Alum 0.05g

[0110] Sodium bicarbonate 1.0g

[0111] Add water to make up 1000ml

[0112]

[0113] Add 24g of rice and 32ml of water into each culture bottle, cover it after mixing, and sterilize; add 2.5ml of the original seed solution to each culture bottle for inoculation. Transfer the seeded Cordyceps militaris culture bottle ...

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Abstract

The invention provides a method for industrialized cultivation of Cordyceps militaris with high content of cordycepin. The method of the present invention uses the screened bacterial strains Cordyceps militaris COB201 and CGMCC No.1823 to carry out optimized cultivation to obtain Cordycepin militaris with a high content of cordycepin. The content of cordycepin in the product reaches 1.7% after testing. The method of the present invention is simple and suitable for large-scale industrialization Production.

Description

Technical field: [0001] The invention belongs to the technical field of food. In particular, it relates to a method for industrialized cultivation of Cordyceps militaris with high content of cordycepin. Background technique: [0002] Cordyceps sinensis is a well-known precious Chinese herbal medicine at home and abroad. Its main components are: cordycepic acid, cordycepin, cordyceps polysaccharides and anti-aging, anti-cancer active substances, etc. Viruses and more. Therefore, the demand for Cordyceps sinensis continues to increase, and the limited natural resources are increasingly exhausted, and artificial cultivation is not easy to succeed. [0003] Cordyceps militaris, also known as Cordyceps militaris. The medicinal ingredients, content and medicinal value of Cordyceps militaris are comparable to those of Cordyceps sinensis, among which the content of cordycepin is 3-5 times that of Cordyceps sinensis, and the contents of other main components are mostly higher than...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 张刚范培萍
Owner 张刚
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