Epothilone biosynthetic gene transcriptional regulation protein and preparation method thereof

A technology of epothilone and gene transcription, which is applied in the field of genetic engineering and can solve the problems of high price and low yield of epothilone
CN109678938AInactive Publication Date: 2019-04-26GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
2 Cites 6 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
Publication Date
2019-04-26
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses epothilone biosynthetic gene transcriptional regulation protein and a preparation method thereof. According to the epothilone biosynthetic gene transcriptional regulation protein and the preparation method thereof, a biotin-labeled epothilone biosynthetic gene promoter P3 fishing method is adopted for the first time to obtain the transcriptional regulation protein of an SOce M4 epothilone biosynthetic gene cluster, and the function of the transcriptional regulation protein is verified by adopting an EMSA experiment. The obtained novel epothilone biosynthetic gene cluster transcriptional regulation protein can lay a molecular biological foundation for the biosynthetic transcriptional regulation of sprangium cellulosum epothilone and the heterologous expression of the epothilone biosynthetic gene cluster, and therefore the efficient biosynthesis of the epothilone and the wide application of the epothilone in the aspect of biological medicine are promoted.
Need to check novelty before this filing date? Find Prior Art

Description

Technical field:

[0001] The invention belongs to the field of genetic engineering, and in particular relates to an epothilone biosynthesis gene transcription regulation protein and a preparation method thereof. Background technique:

[0002] S. cellulosus is a myxobacteria with rich secondary metabolites. Epothilone is a class of 16-membered macrolide compounds with broad-spectrum and high anti-tumor activity produced by S. cellulosus. Due to its better water solubility, lower side effects, broader antitumor activity and good antitumor activity against drug-resistant tumor cells, epothilone has become the most promising compound to replace paclitaxel. At present, ixabepilone, a derivative of epothilone, has been approved for clinical treatment of advanced breast cancer, and achieved good curative effect. Moreover, epothilone B and epothilone D have also been used in clinical trials, but due to the low yield and high price of epothilones, their wide application is limited. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More