Preparation and culturing method of tree branch bacterial strain of pleurotus eryngii and hypsizygus marmoreus

Abstract

The invention relates to the field of agricultural planting and culturing and provides a preparation and culturing method of a tree branch bacterial strain of pleurotus eryngii and hypsizygus marmoreus. The branch bacterial strain includes following raw materials, 10 kg of tree branches, 25 kg of wheat bran, 3.5 kg of gypsum powder, 0.1 kg of quick lime, 0.15 kg of saccharose, 0.01 kg of monopotassium phosphate, 0.01 kg of magnesium sulfate and 70 kg of water. Because a tree branch culture medium is good in permeability, the bacterial strain is rapid in growth of mycelia under cultivation of 23-25 DEG C, wherein the mycelia grows full in a bottle just by about 20 days, which is reduced by about 10 days when compared with common mother strain. After inoculation, the bacterial strain is germinated from multiple points at upper part, middle part and lower part, wherein the mycelia are spread from interior to exterior. Full-bottle time and full-bag time are reduced by about one week. The tree branch bacterial strain, when being used for replacing a wheat grain bacterial strain, can reach 100% in bacteria growth rate and is reduced in spreading time to 50% in a conventional method, namely, the tree branch bacterial strain just needs 25-28 days in bacteria spreading and growing while the wheat grain bacterial strain needs to 60 days to complete the bacteria spreading and growing in normal situation.

Description

technical field [0001] The invention relates to the field of agricultural planting and cultivation, in particular to the production of branch strains. Background technique [0002] The key to the success of artificial cultivation of edible fungi is the strain, and only good strains can achieve high quality and high yield. According to the source and production purpose, mushroom strains are divided into mother species (the original species are called first-level species), original species (the mother species are bred and called second-level species) and cultivated species (the mother species are bred for production and cultivation, called three species) level species). It is expensive to use the mother seed directly for cultivation, and the production of the mother seed requires equipment and technology, and the cost is too high. Breeding original species and cultivated species by yourself can reduce equipment investment, reduce costs, and obtain high-quality bacterial sour...

AI Technical Summary

Problems solved by technology

[0008] However, these traditional methods are likely to cause contamination when transferring the medium, reducing the success rate of inoculation

At the same time, the germination rate of the strains is not high enough, and the common wheat strains normally take 60 days to complete, which is too long and affects work efficiency