Preparation and culturing method of tree branch bacterial strain of pleurotus eryngii and hypsizygus marmoreus

A technology for branch strains and cultivation methods, which is applied in the directions of organic fertilizers, fertilizer mixtures, fertilization devices, etc., can solve the problems of insufficient bacterial growth rate, reduce the success rate of inoculation, affect work efficiency, etc., so as to improve the bacteria removal time. , Increase the success rate of vaccination and reduce the effect of pollution

Inactive Publication Date: 2016-01-13
TIANHUA MODERN AGRI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, these traditional methods are likely to cause contamination when transferring the medium, reducing the success rate of inoculation
At the same time,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for making and cultivating the branches of Pleurotus eryngii seafood mushroom is characterized in that the raw materials are composed of the following components by mass: 10 kg of branches, 25 kg of wheat bran, 3.5 kg of gypsum powder, 0.1 kg of quicklime, 0.15 kg of sucrose, Potassium dihydrogen phosphate 0.01 kilogram, magnesium sulfate 0.01 kilogram, water 70 kilograms, and its manufacturing process steps are:

[0026] a. Pour water into a large pot, then dissolve sucrose, potassium dihydrogen phosphate, magnesium sulfate, and quicklime in water to prepare a nutrient solution. After dissolving, put the branches into a large pot; b. Heat and boil for 30-40 minutes, Randomly extract several branches from the pot, cut them longitudinally with a knife to check the absorption of nutrient solution by the branches, and the water can be absorbed to 2 / 3; c. Take out the branches and roll them on the mixed gypsum powder and wheat bran to make the branches Cover with...

Embodiment 2

[0029] The method for making and cultivating the branches of Pleurotus eryngii seafood mushroom is characterized in that the raw materials are composed of the following components by mass: 10 kg of branches, 25 kg of wheat bran, 3.5 kg of gypsum powder, 0.1 kg of quicklime, 0.15 kg of sucrose, Potassium dihydrogen phosphate 0.01 kilogram, magnesium sulfate 0.01 kilogram, water 70 kilograms, and its manufacturing process steps are:

[0030] a. Pour water into a large pot, then dissolve sucrose, potassium dihydrogen phosphate, magnesium sulfate, and quicklime in water to prepare a nutrient solution. After dissolving, put the branches into the large pot; b. Put the branches into the dissolved nutrients Soak in a large pot of liquid, 8 hours in summer and 12 hours in winter, randomly select several branches from the pot, cut longitudinally with a knife to check the absorption of nutrient solution by the branches, if the water is absorbed to 2 / 3, then it can be; c Take out the bran...

Embodiment 3

[0033] The method for producing and cultivating Pleurotus eryngii seafood mushroom branches is characterized in that the raw materials are composed of the following components by mass: 8 kg of branches, 20 kg of wheat bran, 3 kg of gypsum powder, 0.05 kg of quicklime, 0.1 kg of sucrose, Potassium dihydrogen phosphate 0.005 kg, magnesium sulfate 0.005 kg, water 60 kg, its manufacturing process steps are:

[0034] a. Pour water into a large pot, then dissolve sucrose, potassium dihydrogen phosphate, magnesium sulfate, and quicklime in water to prepare a nutrient solution. After dissolving, put the branches into a large pot; b. Heat and boil for 30-40 minutes, Randomly extract several branches from the pot, cut them longitudinally with a knife to check the absorption of nutrient solution by the branches, and the water can be absorbed to 2 / 3; c. Take out the branches and roll them on the mixed gypsum powder and wheat bran to make the branches Cover with wheat bran; d. Neatly pack ...

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PUM

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Abstract

The invention relates to the field of agricultural planting and culturing and provides a preparation and culturing method of a tree branch bacterial strain of pleurotus eryngii and hypsizygus marmoreus. The branch bacterial strain includes following raw materials, 10 kg of tree branches, 25 kg of wheat bran, 3.5 kg of gypsum powder, 0.1 kg of quick lime, 0.15 kg of saccharose, 0.01 kg of monopotassium phosphate, 0.01 kg of magnesium sulfate and 70 kg of water. Because a tree branch culture medium is good in permeability, the bacterial strain is rapid in growth of mycelia under cultivation of 23-25 DEG C, wherein the mycelia grows full in a bottle just by about 20 days, which is reduced by about 10 days when compared with common mother strain. After inoculation, the bacterial strain is germinated from multiple points at upper part, middle part and lower part, wherein the mycelia are spread from interior to exterior. Full-bottle time and full-bag time are reduced by about one week. The tree branch bacterial strain, when being used for replacing a wheat grain bacterial strain, can reach 100% in bacteria growth rate and is reduced in spreading time to 50% in a conventional method, namely, the tree branch bacterial strain just needs 25-28 days in bacteria spreading and growing while the wheat grain bacterial strain needs to 60 days to complete the bacteria spreading and growing in normal situation.

Description

technical field [0001] The invention relates to the field of agricultural planting and cultivation, in particular to the production of branch strains. Background technique [0002] The key to the success of artificial cultivation of edible fungi is the strain, and only good strains can achieve high quality and high yield. According to the source and production purpose, mushroom strains are divided into mother species (the original species are called first-level species), original species (the mother species are bred and called second-level species) and cultivated species (the mother species are bred for production and cultivation, called three species) level species). It is expensive to use the mother seed directly for cultivation, and the production of the mother seed requires equipment and technology, and the cost is too high. Breeding original species and cultivated species by yourself can reduce equipment investment, reduce costs, and obtain high-quality bacterial sour...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05F11/00
Inventor 黄巧珍
Owner TIANHUA MODERN AGRI CO LTD
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